BBa_R0040
1
p(tetR)
TetR repressible promoter
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
Lutz, R., Bujard, H., <em>Nucleic Acids Research</em> (1997) 25, 1203-1210.
Released HQ 2013
Sequence for pTet inverting regulator driven by the TetR protein.</P>
false
true
_1_
0
24
7
In stock
false
<P> <P>BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.<P>
true
June Rhee, Connie Tao, Ty Thomson, Louis Waldman
annotation1986784
1
BBa_R0040
range1986784
1
1
54
annotation1986787
1
-10
range1986787
1
43
48
annotation1986786
1
TetR 2
range1986786
1
26
44
annotation1986785
1
-35
range1986785
1
20
25
annotation1986783
1
TetR 1
range1986783
1
1
19
BBa_K199000
1
BBa_K199000
CCCUC tRNA Suppressor (Produces Serine)
2009-06-24T11:00:00Z
2015-05-08T01:11:18Z
De Novo synthesis from oligos. Based on the paper by Anderson et al. (http://gcat.davidson.edu/GcatWiki/images/6/6f/AndersonSchultz2002ChemBiol.pdf)
This gene encodes for a 5 base pair tRNA suppressor that suppresses CCCUC, which would normally cause a frame shift mutation.
false
false
_295_
0
5114
9
It's complicated
false
We used the 9 base anticodon loop that is complementary to the 5 base pair codon CCCUC.
false
Leland Taylor
annotation2006688
1
Anti-codon Loop
range2006688
1
44
52
annotation2006689
1
5' Context
range2006689
1
1
11
annotation2006690
1
3' Context
range2006690
1
104
143
BBa_K199090
1
BBa_K199090
R0040:K199000
2009-10-03T11:00:00Z
2015-05-08T01:11:19Z
?
Construction intermediate
false
false
_295_
0
5109
9
It's complicated
false
?
false
Romina Clemente
component2028055
1
BBa_R0040
component2028063
1
BBa_K199000
annotation2028063
1
BBa_K199000
range2028063
1
63
205
annotation2028055
1
BBa_R0040
range2028055
1
1
54
BBa_K199090_sequence
1
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagggatccaattcggagagatgccggagcggctgaacggaccggtctgagggtcaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactgcatatccttagcgaaagctaaggattttttttaagct
BBa_K199000_sequence
1
ggatccaattcggagagatgccggagcggctgaacggaccggtctgagggtcaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactgcatatccttagcgaaagctaaggattttttttaagct
BBa_R0040_sequence
1
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z