##gff-version 3
##sequence-region BBa_K199092 1 1150
BBa_K199092	.	engineered_region	440	1150	.	+	0	ID=BBa_K199034;Name=BBa_K199034
BBa_K199092	.	sequence_feature	1120	1125	.	+	0	ID=annotation2013330;Name=Double Stop;Parent=BBa_K199034
BBa_K199092	.	sequence_feature	1126	1150	.	+	0	ID=annotation2013329;Name=25 bp addition;Parent=BBa_K199034
BBa_K199092	.	sequence_feature	863	868	.	+	0	ID=annotation2011262;Name=Nco1 restriction site;Parent=BBa_K199034
BBa_K199092	.	sequence_feature	443	448	.	+	0	ID=annotation2011261;Name=5 base pair addition;Parent=BBa_K199034
BBa_K199092	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K199092	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K199092	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K199092	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K199092	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K199092	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K199092	.	ribosome_entry_site	422	433	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K199092	.	conserved_region	426	429	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K199092	.	mature_transcript_region	63	205	.	+	0	ID=BBa_K199028;Name=BBa_K199028
BBa_K199092	.	sequence_feature	106	114	.	+	0	ID=annotation2010430;Name=anticodon loop;Parent=BBa_K199028
BBa_K199092	.	sequence_feature	63	73	.	+	0	ID=annotation2010431;Name=5' context;Parent=BBa_K199028
BBa_K199092	.	sequence_feature	166	205	.	+	0	ID=annotation2010432;Name=3;Parent=BBa_K199028
BBa_K199092	.	promoter	214	413	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_K199092	.	non_covalent_binding_site	302	339	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K199092	.	engineered_region	214	413	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K199092	.	non_covalent_binding_site	379	413	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K199092	.	start_codon	386	386	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K199092	.	promoter	350	355	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K199092	.	CDS	214	301	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K199092	.	promoter	374	379	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
>BBa_K199092
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagggatccaattcggagag
atgccggagcggctgaacggaccggtttgaccgacaccggagtaggggcaactctaccgggggttcaaatccccctctc
tccgccactgcatatccttagcgaaagctaaggattttttttaagcttactagagcaatacgcaaaccgcctctccccg
cgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaa
tgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagc
ggataacaatttcacacatactagagaaagaggagaaatactagatgcggtcgcttcctccgaagacgttatcaaagag
ttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtc
cgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtcccc
gcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaa
ggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacg
gtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatggg
ttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaa
gacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaa
ccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtca
ctccaccggtgcttaataacgctgatagtgctagtgtagatcgc