LOCUS       BBa_K199096             1150 bp    DNA     linear       26-Nov-2024
DEFINITION  pTet-CCCUC_tRNA-pLac-RBS-CCCUC_RFP
ACCESSION   BBa_K199096
VERSION     BBa_K199096.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    106..114
                     /label=Anti-codon Loop
     misc_feature    63..73
                     /label=5' Context
     misc_feature    166..205
                     /label=3' Context
     misc_feature    63..205
                     /label=BBa_K199000
     CDS             214..301
                     /label=end of LacI coding region (inactive)
     misc_feature    214..413
                     /label=BBa_R0010
     misc_feature    302..339
                     /label=CAP binding site
     promoter        350..355
                     /label=-35
     promoter        374..379
                     /label=-10
     misc_feature    379..413
                     /label=LacI binding site
     misc_feature    386..386
                     /label=start
     promoter        214..413
                     /label=BBa_R0010
     misc_feature    426..429
                     /label=conserved
     RBS             422..433
                     /label=BBa_B0034
     misc_feature    443..447
                     /label=CCCUC 5 Base Addition
     misc_feature    863..868
                     /label=Nco1 restriction site
     misc_feature    1126..1150
                     /label=25 bp addition
     misc_feature    1120..1125
                     /label=Double Stop
     misc_feature    440..1150
                     /label=BBa_K199011
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agggatccaa ttcggagaga tgccggagcg gctgaacgga ccggtctgag ggtcaccgga
      121 gtaggggcaa ctctaccggg ggttcaaatc cccctctctc cgccactgca tatccttagc
      181 gaaagctaag gatttttttt aagcttacta gagcaatacg caaaccgcct ctccccgcgc
      241 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
      301 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
      361 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acatactaga
      421 gaaagaggag aaatactaga tgccctcgct tcctccgaag acgttatcaa agagttcatg
      481 cgtttcaaag ttcgtatgga aggttccgtt aacggtcacg agttcgaaat cgaaggtgaa
      541 ggtgaaggtc gtccgtacga aggtacccag accgctaaac tgaaagttac caaaggtggt
      601 ccgctgccgt tcgcttggga catcctgtcc ccgcagttcc agtacggttc caaagcttac
      661 gttaaacacc cggctgacat cccggactac ctgaaactgt ccttcccgga aggtttcaaa
      721 tgggaacgtg ttatgaactt cgaagacggt ggtgttgtta ccgttaccca ggactcctcc
      781 ctgcaagacg gtgagttcat ctacaaagtt aaactgcgtg gtaccaactt cccgtccgac
      841 ggtccggtta tgcagaaaaa aaccatgggt tgggaagctt ccaccgaacg tatgtacccg
      901 gaagacggtg ctctgaaagg tgaaatcaaa atgcgtctga aactgaaaga cggtggtcac
      961 tacgacgctg aagttaaaac cacctacatg gctaaaaaac cggttcagct gccgggtgct
     1021 tacaaaaccg acatcaaact ggacatcacc tcccacaacg aagactacac catcgttgaa
     1081 cagtacgaac gtgctgaagg tcgtcactcc accggtgctt aataacgctg atagtgctag
     1141 tgtagatcgc
//