LOCUS       BBa_K199097             1150 bp    DNA     linear       28-Jun-2024
DEFINITION  pLac+RBS+CCCUC_RFP+pTet+CCCUC_tRNA
ACCESSION   BBa_K199097
VERSION     BBa_K199097.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    230..234
                     /label=CCCUC 5 Base Addition
     misc_feature    650..655
                     /label=Nco1 restriction site
     misc_feature    913..937
                     /label=25 bp addition
     misc_feature    907..912
                     /label=Double Stop
     misc_feature    227..937
                     /label=BBa_K199011
     misc_feature    946..964
                     /label=TetR 1
     misc_feature    946..999
                     /label=BBa_R0040
     promoter        965..970
                     /label=-35
     misc_feature    971..989
                     /label=TetR 2
     promoter        988..993
                     /label=-10
     promoter        946..999
                     /label=BBa_R0040
     misc_feature    1051..1059
                     /label=Anti-codon Loop
     misc_feature    1008..1018
                     /label=5' Context
     misc_feature    1111..1150
                     /label=3' Context
     misc_feature    1008..1150
                     /label=BBa_K199000
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgc cctcgcttcc
      241 tccgaagacg ttatcaaaga gttcatgcgt ttcaaagttc gtatggaagg ttccgttaac
      301 ggtcacgagt tcgaaatcga aggtgaaggt gaaggtcgtc cgtacgaagg tacccagacc
      361 gctaaactga aagttaccaa aggtggtccg ctgccgttcg cttgggacat cctgtccccg
      421 cagttccagt acggttccaa agcttacgtt aaacacccgg ctgacatccc ggactacctg
      481 aaactgtcct tcccggaagg tttcaaatgg gaacgtgtta tgaacttcga agacggtggt
      541 gttgttaccg ttacccagga ctcctccctg caagacggtg agttcatcta caaagttaaa
      601 ctgcgtggta ccaacttccc gtccgacggt ccggttatgc agaaaaaaac catgggttgg
      661 gaagcttcca ccgaacgtat gtacccggaa gacggtgctc tgaaaggtga aatcaaaatg
      721 cgtctgaaac tgaaagacgg tggtcactac gacgctgaag ttaaaaccac ctacatggct
      781 aaaaaaccgg ttcagctgcc gggtgcttac aaaaccgaca tcaaactgga catcacctcc
      841 cacaacgaag actacaccat cgttgaacag tacgaacgtg ctgaaggtcg tcactccacc
      901 ggtgcttaat aacgctgata gtgctagtgt agatcgctac tagagtccct atcagtgata
      961 gagattgaca tccctatcag tgatagagat actgagcact actagaggga tccaattcgg
     1021 agagatgccg gagcggctga acggaccggt ctgagggtca ccggagtagg ggcaactcta
     1081 ccgggggttc aaatccccct ctctccgcca ctgcatatcc ttagcgaaag ctaaggattt
     1141 tttttaagct
//