LOCUS       BBa_K199098             1316 bp    DNA     linear       04-Jul-2024
DEFINITION  CUACC with RFP Reporter
ACCESSION   BBa_K199098
VERSION     BBa_K199098.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    254..258
                     /label=5nt anti codon
     misc_feature    209..351
                     /label=BBa_K199045
     CDS             360..447
                     /label=end of LacI coding region (inactive)
     misc_feature    360..559
                     /label=BBa_R0010
     misc_feature    448..485
                     /label=CAP binding site
     promoter        496..501
                     /label=-35
     promoter        520..525
                     /label=-10
     misc_feature    525..559
                     /label=LacI binding site
     misc_feature    532..532
                     /label=start
     promoter        360..559
                     /label=BBa_R0010
     misc_feature    572..575
                     /label=conserved
     RBS             568..579
                     /label=BBa_B0034
     misc_feature    1029..1029
                     /label=NcoI Restriction Site
     misc_feature    609..613
                     /label=5-bp CUACC Addition
     misc_feature    1292..1316
                     /label=25 bp addition
     misc_feature    588..1316
                     /label=BBa_K199075
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagaggg atccaattcg gagagatgcc ggagcggctg
      241 aacggaccgg tgtggtagaa accggagtag gggcaactct accgggggtt caaatccccc
      301 tctctccgcc actgcatatc cttagcgaaa gctaaggatt ttttttaagc ttactagagc
      361 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
      421 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
      481 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
      541 cggataacaa tttcacacat actagagaaa gaggagaaat actagagaaa gaggagaaat
      601 actagatgct accgcttcct ccgaagacgt tatcaaagag ttcatgcgtt tcaaagttcg
      661 tatggaaggt tccgttaacg gtcacgagtt cgaaatcgaa ggtgaaggtg aaggtcgtcc
      721 gtacgaaggt acccagaccg ctaaactgaa agttaccaaa ggtggtccgc tgccgttcgc
      781 ttgggacatc ctgtccccgc agttccagta cggttccaaa gcttacgtta aacacccggc
      841 tgacatcccg gactacctga aactgtcctt cccggaaggt ttcaaatggg aacgtgttat
      901 gaacttcgaa gacggtggtg ttgttaccgt tacccaggac tcctccctgc aagacggtga
      961 gttcatctac aaagttaaac tgcgtggtac caacttcccg tccgacggtc cggttatgca
     1021 gaaaaaaacc atgggttggg aagcttccac cgaacgtatg tacccggaag acggtgctct
     1081 gaaaggtgaa atcaaaatgc gtctgaaact gaaagacggt ggtcactacg acgctgaagt
     1141 taaaaccacc tacatggcta aaaaaccggt tcagctgccg ggtgcttaca aaaccgacat
     1201 caaactggac atcacctccc acaacgaaga ctacaccatc gttgaacagt acgaacgtgc
     1261 tgaaggtcgt cactccaccg gtgcttaata acgctgatag tgctagtgta gatcgc
//