LOCUS       BBa_K199100             1316 bp    DNA     linear       26-Dec-2024
DEFINITION  pLac-RBS-CUACC-RFP and pLac-CUACC Suppressor tRNA
ACCESSION   BBa_K199100
VERSION     BBa_K199100.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    670..670
                     /label=NcoI Restriction Site
     misc_feature    250..254
                     /label=5-bp CUACC Addition
     misc_feature    933..957
                     /label=25 bp addition
     misc_feature    229..957
                     /label=BBa_K199075
     CDS             966..1053
                     /label=end of LacI coding region (inactive)
     misc_feature    966..1165
                     /label=BBa_R0010
     misc_feature    1054..1091
                     /label=CAP binding site
     promoter        1102..1107
                     /label=-35
     promoter        1126..1131
                     /label=-10
     misc_feature    1131..1165
                     /label=LacI binding site
     misc_feature    1138..1138
                     /label=start
     promoter        966..1165
                     /label=BBa_R0010
     misc_feature    1219..1223
                     /label=5nt anti codon
     misc_feature    1174..1316
                     /label=BBa_K199045
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagagaa agaggagaaa
      241 tactagatgc taccgcttcc tccgaagacg ttatcaaaga gttcatgcgt ttcaaagttc
      301 gtatggaagg ttccgttaac ggtcacgagt tcgaaatcga aggtgaaggt gaaggtcgtc
      361 cgtacgaagg tacccagacc gctaaactga aagttaccaa aggtggtccg ctgccgttcg
      421 cttgggacat cctgtccccg cagttccagt acggttccaa agcttacgtt aaacacccgg
      481 ctgacatccc ggactacctg aaactgtcct tcccggaagg tttcaaatgg gaacgtgtta
      541 tgaacttcga agacggtggt gttgttaccg ttacccagga ctcctccctg caagacggtg
      601 agttcatcta caaagttaaa ctgcgtggta ccaacttccc gtccgacggt ccggttatgc
      661 agaaaaaaac catgggttgg gaagcttcca ccgaacgtat gtacccggaa gacggtgctc
      721 tgaaaggtga aatcaaaatg cgtctgaaac tgaaagacgg tggtcactac gacgctgaag
      781 ttaaaaccac ctacatggct aaaaaaccgg ttcagctgcc gggtgcttac aaaaccgaca
      841 tcaaactgga catcacctcc cacaacgaag actacaccat cgttgaacag tacgaacgtg
      901 ctgaaggtcg tcactccacc ggtgcttaat aacgctgata gtgctagtgt agatcgctac
      961 tagagcaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     1021 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     1081 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     1141 tgtgagcgga taacaatttc acacatacta gagggatcca attcggagag atgccggagc
     1201 ggctgaacgg accggtgtgg tagaaaccgg agtaggggca actctaccgg gggttcaaat
     1261 ccccctctct ccgccactgc atatccttag cgaaagctaa ggattttttt taagct
//