##gff-version 3
##sequence-region BBa_K199100 1 1316
BBa_K199100	.	ribosome_entry_site	209	220	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K199100	.	conserved_region	213	216	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K199100	.	promoter	966	1165	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_K199100	.	CDS	966	1053	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K199100	.	non_covalent_binding_site	1131	1165	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K199100	.	start_codon	1138	1138	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K199100	.	engineered_region	966	1165	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K199100	.	promoter	1102	1107	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K199100	.	non_covalent_binding_site	1054	1091	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K199100	.	promoter	1126	1131	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K199100	.	engineered_region	229	957	.	+	0	ID=BBa_K199075;Name=BBa_K199075
BBa_K199100	.	sequence_feature	250	254	.	+	0	ID=annotation2023122;Name=5-bp CUACC Addition;Parent=BBa_K199075
BBa_K199100	.	sequence_feature	670	670	.	+	0	ID=annotation2023121;Name=NcoI Restriction Site;Parent=BBa_K199075
BBa_K199100	.	sequence_feature	933	957	.	+	0	ID=annotation2023123;Name=25 bp addition;Parent=BBa_K199075
BBa_K199100	.	mature_transcript_region	1174	1316	.	+	0	ID=BBa_K199045;Name=BBa_K199045
BBa_K199100	.	sequence_feature	1219	1223	.	+	0	ID=annotation2011866;Name=5nt anti codon;Parent=BBa_K199045
BBa_K199100	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_K199100	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K199100	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K199100	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K199100	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K199100	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K199100	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K199100	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
>BBa_K199100
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagagaaagaggag
aaatactagatgctaccgcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgt
taacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagtt
accaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaac
acccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaaga
cggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtacc
aacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaag
acggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccac
ctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaa
gactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagt
gtagatcgctactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacagg
tttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttac
actttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagggatccaattcg
gagagatgccggagcggctgaacggaccggtgtggtagaaaccggagtaggggcaactctaccgggggttcaaatcccc
ctctctccgccactgcatatccttagcgaaagctaaggattttttttaagct