BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_J23101
1
BBa_J23101
constitutive promoter family member
2006-08-03T11:00:00Z
2015-08-31T04:08:40Z
later
Released HQ 2013
later
false
true
_52_
0
483
95
In stock
true
N/A
true
John Anderson
BBa_K1991000
1
BBa_K1991000
AOX1 (Alcohol oxidase)
2016-10-11T11:00:00Z
2016-10-12T02:37:13Z
Pichia pastoris
There are two Alcohol oxidase (AOX) genes, AOX1 (CAY72092.1) and AOX2 (CAY71377.1), on chromosome 4 in Pichia pastoris. AOX was utilized to provide carbon source and energy for the methylotrophic yeast by metabolizing primary alcohol like methanol.
false
false
_2458_
31417
31417
9
false
No
false
Chen, Pei-En
annotation2520492
1
AOX1
range2520492
1
1
1992
BBa_K1991002
1
BBa_K1991002
Pcons-RBS-AOX1 (Alcohol Oxidase)
2016-10-11T11:00:00Z
2016-10-12T03:08:18Z
Pichia pastoris
There are two Alcohol oxidase (AOX) genes, AOX1 (CAY72092.1) and AOX2 (CAY71377.1), on chromosome 4 in Pichia pastoris. AOX was utilized to provide carbon source and energy for the methylotrophic yeast by metabolizing primary alcohol like methanol.
false
false
_2458_
31417
31417
9
false
No
false
Chen, Pei-En
component2493521
1
BBa_J23101
component2493523
1
BBa_B0034
component2493524
1
BBa_K1991000
annotation2493523
1
BBa_B0034
range2493523
1
44
55
annotation2493524
1
BBa_K1991000
range2493524
1
62
2053
annotation2493521
1
BBa_J23101
range2493521
1
1
35
BBa_B0034_sequence
1
aaagaggagaaa
BBa_J23101_sequence
1
tttacagctagctcagtcctaggtattatgctagc
BBa_K1991002_sequence
1
tttacagctagctcagtcctaggtattatgctagctactagagaaagaggagaaatactagatggctattccggaagagttcgatatcttagtgttgggtggaggttcgagcgggtcgtgtattgccggtcgcttggcgaatctggaccacagcttaaaagtaggtcttattgaagcaggtgaaaataacctgaacaacccgtgggtatacttacccggaatttacccacgtaatatgaaacttgacagtaagacggcatcgttctatactagcaatccttctccacatctgaacggtcgccgtaccattgtgccatgcgctaacgtgcttggcggtgggtcaagtattaactttatgatgtatacgcgtggtagtgcttcagactacgatgactttcaagccgaaggttggaaaacgaaagacctgcttcctttaatgaagaaaaccgaaacctaccagcgtgcgtgtaacaatcctgacatccacggattcgaaggaccaatcaaagtgagtttcggcaattacacatatccggtatgtcaggatttcttacgcgcatcggaatcgcaaggtatcccgtatgttgacgatctggaagacctggtgaccgctcacggtgctgaacactggttaaagtggatcaatcgcgatacgggtcgtcgctcagatagtgctcacgcattcgttcatagtaccatgcgcaatcatgataatttgtaccttatctgtaacacgaaagtcgacaagattatcgtggaggacggtcgtgcagccgcggtacgtaccgtgcctagtaaaccccttaatccaaagaaaccctcgcacaaaatctatcgcgcacgcaaacaaattgtcttatcgtgtgggacgatttcctctccattagtccttcagcgctcgggtttcggggacccgattaaattgcgtgcggctggtgttaagcccttagttaatcttcctggagttggccgcaactttcaagatcactactgcttcttctctccgtatcgcatcaagccacaatacgagtctttcgatgactttgtccgcggtgatgccgaaatccagaagcgtgtatttgatcagtggtatgcaaacggaacgggtccccttgcaacaaacggtatcgaggccggagtgaagattcgtcccactcctgaagagttaagccagatggacgagagcttccaggaaggctatcgtgaatacttcgaagacaaaccggacaagccggtcatgcactactccatcatcgcaggtttcttcggcgatcacaccaagattccgcctgggaaatatatgactatgttccacttccttgaatatccattctctcgtgggtcaattcatattacgtctcccgatccatatgccgcacccgactttgaccctggattcatgaacgacgaacgcgacatggcaccgatggtttgggcatataagaaatcccgcgaaactgcacgccgtatggatcattttgctggcgaagtgaccagccaccatccattatttccctattcgtcggaggcccgtgcattggaaatggatctggagacgtccaacgcgtatggagggcccttaaacttgagcgctggcctggctcatggctcctggacgcaacctctgaagaagcccactgcaaagaacgaggggcatgtaacctcaaatcaggtagaacttcacccggacatcgaatatgatgaggaagatgataaagccatcgagaactatatccgtgaacataccgagaccacctggcattgcttaggcacttgcagtattgggccgcgtgaaggcagtaaaatcgtgaaatggggaggggttttggatcaccgctccaacgtctacggcgttaaagggctgaaagtcggggatttatctgtatgtcctgacaacgtcggctgtaacacctacacaacggcattgttgattggagagaaaacggctacactggttggggaagaccttggttattctggggaggcgttggatatgacggtcccacaatttaagttaggaacatatgaaaagaccggccttgcgcgcttttga
BBa_K1991000_sequence
1
atggctattccggaagagttcgatatcttagtgttgggtggaggttcgagcgggtcgtgtattgccggtcgcttggcgaatctggaccacagcttaaaagtaggtcttattgaagcaggtgaaaataacctgaacaacccgtgggtatacttacccggaatttacccacgtaatatgaaacttgacagtaagacggcatcgttctatactagcaatccttctccacatctgaacggtcgccgtaccattgtgccatgcgctaacgtgcttggcggtgggtcaagtattaactttatgatgtatacgcgtggtagtgcttcagactacgatgactttcaagccgaaggttggaaaacgaaagacctgcttcctttaatgaagaaaaccgaaacctaccagcgtgcgtgtaacaatcctgacatccacggattcgaaggaccaatcaaagtgagtttcggcaattacacatatccggtatgtcaggatttcttacgcgcatcggaatcgcaaggtatcccgtatgttgacgatctggaagacctggtgaccgctcacggtgctgaacactggttaaagtggatcaatcgcgatacgggtcgtcgctcagatagtgctcacgcattcgttcatagtaccatgcgcaatcatgataatttgtaccttatctgtaacacgaaagtcgacaagattatcgtggaggacggtcgtgcagccgcggtacgtaccgtgcctagtaaaccccttaatccaaagaaaccctcgcacaaaatctatcgcgcacgcaaacaaattgtcttatcgtgtgggacgatttcctctccattagtccttcagcgctcgggtttcggggacccgattaaattgcgtgcggctggtgttaagcccttagttaatcttcctggagttggccgcaactttcaagatcactactgcttcttctctccgtatcgcatcaagccacaatacgagtctttcgatgactttgtccgcggtgatgccgaaatccagaagcgtgtatttgatcagtggtatgcaaacggaacgggtccccttgcaacaaacggtatcgaggccggagtgaagattcgtcccactcctgaagagttaagccagatggacgagagcttccaggaaggctatcgtgaatacttcgaagacaaaccggacaagccggtcatgcactactccatcatcgcaggtttcttcggcgatcacaccaagattccgcctgggaaatatatgactatgttccacttccttgaatatccattctctcgtgggtcaattcatattacgtctcccgatccatatgccgcacccgactttgaccctggattcatgaacgacgaacgcgacatggcaccgatggtttgggcatataagaaatcccgcgaaactgcacgccgtatggatcattttgctggcgaagtgaccagccaccatccattatttccctattcgtcggaggcccgtgcattggaaatggatctggagacgtccaacgcgtatggagggcccttaaacttgagcgctggcctggctcatggctcctggacgcaacctctgaagaagcccactgcaaagaacgaggggcatgtaacctcaaatcaggtagaacttcacccggacatcgaatatgatgaggaagatgataaagccatcgagaactatatccgtgaacataccgagaccacctggcattgcttaggcacttgcagtattgggccgcgtgaaggcagtaaaatcgtgaaatggggaggggttttggatcaccgctccaacgtctacggcgttaaagggctgaaagtcggggatttatctgtatgtcctgacaacgtcggctgtaacacctacacaacggcattgttgattggagagaaaacggctacactggttggggaagaccttggttattctggggaggcgttggatatgacggtcccacaatttaagttaggaacatatgaaaagaccggccttgcgcgcttttga
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z