BBa_K1991001 1 BBa_K1991001 AOX2 (Alcohol Oxidase) 2016-10-11T11:00:00Z 2016-10-12T02:45:19Z Pichia pastoris There are two Alcohol oxidase (AOX) genes, AOX1 (CAY72092.1) and AOX2 (CAY71377.1), on chromosome 4 in Pichia pastoris. AOX was utilized to provide carbon source and energy for the methylotrophic yeast by metabolizing primary alcohol like methanol. false false _2458_ 31417 31417 9 false No false Chen, Pei-En annotation2521245 1 AOX2 range2521245 1 1 1992 BBa_J23101 1 BBa_J23101 constitutive promoter family member 2006-08-03T11:00:00Z 2015-08-31T04:08:40Z later Released HQ 2013 later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K1991003 1 BBa_K1991003 Pcons-RBS-AOX2 2016-10-11T11:00:00Z 2016-10-12T03:13:07Z Pichia pastoris There are two Alcohol oxidase (AOX) genes, AOX1 (CAY72092.1) and AOX2 (CAY71377.1), on chromosome 4 in Pichia pastoris. AOX was utilized to provide carbon source and energy for the methylotrophic yeast by metabolizing primary alcohol like methanol. false false _2458_ 31417 31417 9 false No false Chen, Pei-En component2493529 1 BBa_K1991001 component2493526 1 BBa_J23101 component2493528 1 BBa_B0034 annotation2493526 1 BBa_J23101 range2493526 1 1 35 annotation2493529 1 BBa_K1991001 range2493529 1 62 2053 annotation2493528 1 BBa_B0034 range2493528 1 44 55 BBa_B0034_sequence 1 aaagaggagaaa BBa_K1991001_sequence 1 atggccatccctgaggaatttgacattttggtcttaggtggcgggtcttccggctcttgtattgcaggacgcttagcgaacctggatcactcgttaaaggttggattaatcgaggctggcgagaacaacttaaataacccgtgggtttatctgccagggatctatccgcgtaatatgaaattagattcaaaaacggcgtctttctacacttctaacccgtctcctcatttaaacggacgccgcgctatcgttccatgtgccaatattttgggaggtgggagttctattaatttcatgatgtatacccgtgggtcggcgtctgattacgacgactttgaggctgaaggttggaaaacaaaggaccttttgccgcttatgaagaaaacagaaacttatcagcgcgcttgtaacaaccccgaaatccacggttttgagggcccaattaaagtttcttttgggaattacacttatccagtatgtcaggatttcttacgcgccacagaatcccagggtattccgtatgtggatgacttagaagaccttgtaaccgcacatggtgccgagcactggttaaaatggattaaccgtgacacaggtcgccgctcggattctgctcatgccttcgtacatagcaccatgcgtaaccatgataatctgtacctgatttgtaataccaaggttgataaaattattgtcgaagacggacgtgcagccgcagtacgtacagtccctagcaaaccgctgaatgccaagaaaccaacgcataaggtctatcgtgcgcgcaaacaaattgtcttaagctgcggtactatttctagcccgcttgtgttgcagcgttctgggtttggcgaccccattaagttgcgtgctgcgggtgtcaagccccttgtgaaccttcccggcgtcgggcgcaatttccaggaccactattgctttttcagcccgtaccgcatcaaaccacagtatgagtcgtttgatgatttcgtgcgcggggatgccaatatccagaaaaaggtatttgatcagtggtatgctaatggaactggtcccttggccacaaacggtatcgaggcaggggtaaagatccgccctaccccagaggagttaagccaaatggatgaatcattccaggaaggttaccgcgagtattttgaagacaagccggataagccggttatgcactatagtattatcgccggtttcttcggcgatcataccaaaattccgcctggaaagtatatgacgatgtttcatttcttggagtaccctttctcccgcgggtcgatccacatcacgtccccggacccctatgcgaccccggattttgatccggggttcatgaacgatgaacgcgatatggcacctatggtgtggtcatacaagaaatcacgtgagacggcacgcaagatggaccatttcgctggcgaggtgacgtcccatcatcctcttttcccatacagttcggaggcccgtgcgtacgagatggatcttgaaacgagtaatgcgtacggaggtccgctgaacttaactgccgggttagcgcatggctcgtggacgcaaccattaaagaaaccggcaggacgcaatgaggggcacgtgacgtcaaaccaggtggaattacatccagacattgaatacgacgaggaagacgataaagcgattgagaactacatccgtgagcacacagaaactacttggcactgtttgggtacttgctcgatcggtccgcgtgaaggctcaaagatcgttaaatggggtggcgtcttggatcatcgtagtaatgtatacggtgtgaaaggtcttaaggtaggtgatctttccgtgtgcccagacaacgttggctgtaatacttacactacagctctgttaatcggtgagaagactgcgacattggttggtgaggatcttggctataccggcgaggcattagacatgaccgttcctcaatttaaattgggaacttacgagaagacgggcttagcgcgcttctga BBa_J23101_sequence 1 tttacagctagctcagtcctaggtattatgctagc BBa_K1991003_sequence 1 tttacagctagctcagtcctaggtattatgctagctactagagaaagaggagaaatactagatggccatccctgaggaatttgacattttggtcttaggtggcgggtcttccggctcttgtattgcaggacgcttagcgaacctggatcactcgttaaaggttggattaatcgaggctggcgagaacaacttaaataacccgtgggtttatctgccagggatctatccgcgtaatatgaaattagattcaaaaacggcgtctttctacacttctaacccgtctcctcatttaaacggacgccgcgctatcgttccatgtgccaatattttgggaggtgggagttctattaatttcatgatgtatacccgtgggtcggcgtctgattacgacgactttgaggctgaaggttggaaaacaaaggaccttttgccgcttatgaagaaaacagaaacttatcagcgcgcttgtaacaaccccgaaatccacggttttgagggcccaattaaagtttcttttgggaattacacttatccagtatgtcaggatttcttacgcgccacagaatcccagggtattccgtatgtggatgacttagaagaccttgtaaccgcacatggtgccgagcactggttaaaatggattaaccgtgacacaggtcgccgctcggattctgctcatgccttcgtacatagcaccatgcgtaaccatgataatctgtacctgatttgtaataccaaggttgataaaattattgtcgaagacggacgtgcagccgcagtacgtacagtccctagcaaaccgctgaatgccaagaaaccaacgcataaggtctatcgtgcgcgcaaacaaattgtcttaagctgcggtactatttctagcccgcttgtgttgcagcgttctgggtttggcgaccccattaagttgcgtgctgcgggtgtcaagccccttgtgaaccttcccggcgtcgggcgcaatttccaggaccactattgctttttcagcccgtaccgcatcaaaccacagtatgagtcgtttgatgatttcgtgcgcggggatgccaatatccagaaaaaggtatttgatcagtggtatgctaatggaactggtcccttggccacaaacggtatcgaggcaggggtaaagatccgccctaccccagaggagttaagccaaatggatgaatcattccaggaaggttaccgcgagtattttgaagacaagccggataagccggttatgcactatagtattatcgccggtttcttcggcgatcataccaaaattccgcctggaaagtatatgacgatgtttcatttcttggagtaccctttctcccgcgggtcgatccacatcacgtccccggacccctatgcgaccccggattttgatccggggttcatgaacgatgaacgcgatatggcacctatggtgtggtcatacaagaaatcacgtgagacggcacgcaagatggaccatttcgctggcgaggtgacgtcccatcatcctcttttcccatacagttcggaggcccgtgcgtacgagatggatcttgaaacgagtaatgcgtacggaggtccgctgaacttaactgccgggttagcgcatggctcgtggacgcaaccattaaagaaaccggcaggacgcaatgaggggcacgtgacgtcaaaccaggtggaattacatccagacattgaatacgacgaggaagacgataaagcgattgagaactacatccgtgagcacacagaaactacttggcactgtttgggtacttgctcgatcggtccgcgtgaaggctcaaagatcgttaaatggggtggcgtcttggatcatcgtagtaatgtatacggtgtgaaaggtcttaaggtaggtgatctttccgtgtgcccagacaacgttggctgtaatacttacactacagctctgttaatcggtgagaagactgcgacattggttggtgaggatcttggctataccggcgaggcattagacatgaccgttcctcaatttaaattgggaacttacgagaagacgggcttagcgcgcttctga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z