BBa_K1991005 1 BBa_K1991005 LO-AOX1-His (Lpp-OmpA-Alcohol Oxidase) 2016-10-11T11:00:00Z 2016-10-12T02:57:28Z E. coli (Lpp-OmpA) & Pichia pastoris (AOX) A study showed that Lpp-OmpA (LO) hybrid can direct the heterologous protein GFP to the external surface of E. coli (Enzyme Microb Technol. 2001). Bacillus lipase (J Microbiol. 2014) and Fungi xylanase (Curr Microbiol. 2015) were demonstrated to be displayed on the cell surface of E. coli and maintained the functional enzyme activities. We???d like to display the AOX enzyme on bacterial surface by fusing with Lpp-OmpA and apply to the electrochemical analyzer by depositing on the test strip. false false _2458_ 31417 31417 9 false No false Chen, Pei-En annotation2521894 1 T7 terminator range2521894 1 2537 2583 annotation2521819 1 AOX1 range2521819 1 442 2430 annotation2521818 1 Lpp-OmpA range2521818 1 1 441 annotation2521883 1 6X His Tag range2521883 1 2452 2469 BBa_K1991005_sequence 1 atgaaagcaaccaagctggttctgggtgccgtgattctgggcagtaccctgttagcaggttgttctagcaatgccaaaatcgaccaaggcatcaacaacaatggcccgacccacgaaaaccagctgggtgccggtgcctttggtggttatcaggtgaacccgtacgtgggctttgaaatgggctatgattggctgggccgcatgccgtacaaaggcagtgtggagaacggcgcctataaagcacagggcgtgcagctgacagcaaaactgggctaccctattaccgacgacctggacatctacacacgcttaggcggcatggtgtggcgcgccgataccaagagcaacgtgtacggcaagaaccacgataccggcgtgagtccggtgtttgccggcggtgtggagtatgcaatcaccccggaaattgccacacgtggatccatggctattccggaagagttcgatatcttagtgttgggtggaggttcgagcgggtcgtgtattgccggtcgcttggcgaatctggaccacagcttaaaagtaggtcttattgaagcaggtgaaaataacctgaacaacccgtgggtatacttacccggaatttacccacgtaatatgaaacttgacagtaagacggcatcgttctatactagcaatccttctccacatctgaacggtcgccgtaccattgtgccatgcgctaacgtgcttggcggtgggtcaagtattaactttatgatgtatacgcgtggtagtgcttcagactacgatgactttcaagccgaaggttggaaaacgaaagacctgcttcctttaatgaagaaaaccgaaacctaccagcgtgcgtgtaacaatcctgacatccacggattcgaaggaccaatcaaagtgagtttcggcaattacacatatccggtatgtcaggatttcttacgcgcatcggaatcgcaaggtatcccgtatgttgacgatctggaagacctggtgaccgctcacggtgctgaacactggttaaagtggatcaatcgcgatacgggtcgtcgctcagatagtgctcacgcattcgttcatagtaccatgcgcaatcatgataatttgtaccttatctgtaacacgaaagtcgacaagattatcgtggaggacggtcgtgcagccgcggtacgtaccgtgcctagtaaaccccttaatccaaagaaaccctcgcacaaaatctatcgcgcacgcaaacaaattgtcttatcgtgtgggacgatttcctctccattagtccttcagcgctcgggtttcggggacccgattaaattgcgtgcggctggtgttaagcccttagttaatcttcctggagttggccgcaactttcaagatcactactgcttcttctctccgtatcgcatcaagccacaatacgagtctttcgatgactttgtccgcggtgatgccgaaatccagaagcgtgtatttgatcagtggtatgcaaacggaacgggtccccttgcaacaaacggtatcgaggccggagtgaagattcgtcccactcctgaagagttaagccagatggacgagagcttccaggaaggctatcgtgaatacttcgaagacaaaccggacaagccggtcatgcactactccatcatcgcaggtttcttcggcgatcacaccaagattccgcctgggaaatatatgactatgttccacttccttgaatatccattctctcgtgggtcaattcatattacgtctcccgatccatatgccgcacccgactttgaccctggattcatgaacgacgaacgcgacatggcaccgatggtttgggcatataagaaatcccgcgaaactgcacgccgtatggatcattttgctggcgaagtgaccagccaccatccattatttccctattcgtcggaggcccgtgcattggaaatggatctggagacgtccaacgcgtatggagggcccttaaacttgagcgctggcctggctcatggctcctggacgcaacctctgaagaagcccactgcaaagaacgaggggcatgtaacctcaaatcaggtagaacttcacccggacatcgaatatgatgaggaagatgataaagccatcgagaactatatccgtgaacataccgagaccacctggcattgcttaggcacttgcagtattgggccgcgtgaaggcagtaaaatcgtgaaatggggaggggttttggatcaccgctccaacgtctacggcgttaaagggctgaaagtcggggatttatctgtatgtcctgacaacgtcggctgtaacacctacacaacggcattgttgattggagagaaaacggctacactggttggggaagaccttggttattctggggaggcgttggatatgacggtcccacaatttaagttaggaacatatgaaaagaccggccttgcgcgctttaagcttgcggccgcactcgagcaccaccaccaccaccactgagatccggctgctaacaaagcccgaaaggaagctgagttggctgctgccaccgctgagcaataactagcataaccccttggggcctctaaacgggtcttgaggggttttttgctgaaaggaggaactata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z