LOCUS       BBa_K1991008            2662 bp    DNA     linear       14-Nov-2024
DEFINITION  Pcons-RBS-LO-AOX1-His
ACCESSION   BBa_K1991008
VERSION     BBa_K1991008.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23101
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             62..502
                     /label=Lpp-OmpA
     CDS             503..2491
                     /label=AOX1
     misc_feature    2513..2530
                     /label=6X His Tag
     misc_feature    2598..2644
                     /label=T7 terminator
     CDS             62..2662
                     /label=BBa_K1991005
ORIGIN
        1 tttacagcta gctcagtcct aggtattatg ctagctacta gagaaagagg agaaatacta
       61 gatgaaagca accaagctgg ttctgggtgc cgtgattctg ggcagtaccc tgttagcagg
      121 ttgttctagc aatgccaaaa tcgaccaagg catcaacaac aatggcccga cccacgaaaa
      181 ccagctgggt gccggtgcct ttggtggtta tcaggtgaac ccgtacgtgg gctttgaaat
      241 gggctatgat tggctgggcc gcatgccgta caaaggcagt gtggagaacg gcgcctataa
      301 agcacagggc gtgcagctga cagcaaaact gggctaccct attaccgacg acctggacat
      361 ctacacacgc ttaggcggca tggtgtggcg cgccgatacc aagagcaacg tgtacggcaa
      421 gaaccacgat accggcgtga gtccggtgtt tgccggcggt gtggagtatg caatcacccc
      481 ggaaattgcc acacgtggat ccatggctat tccggaagag ttcgatatct tagtgttggg
      541 tggaggttcg agcgggtcgt gtattgccgg tcgcttggcg aatctggacc acagcttaaa
      601 agtaggtctt attgaagcag gtgaaaataa cctgaacaac ccgtgggtat acttacccgg
      661 aatttaccca cgtaatatga aacttgacag taagacggca tcgttctata ctagcaatcc
      721 ttctccacat ctgaacggtc gccgtaccat tgtgccatgc gctaacgtgc ttggcggtgg
      781 gtcaagtatt aactttatga tgtatacgcg tggtagtgct tcagactacg atgactttca
      841 agccgaaggt tggaaaacga aagacctgct tcctttaatg aagaaaaccg aaacctacca
      901 gcgtgcgtgt aacaatcctg acatccacgg attcgaagga ccaatcaaag tgagtttcgg
      961 caattacaca tatccggtat gtcaggattt cttacgcgca tcggaatcgc aaggtatccc
     1021 gtatgttgac gatctggaag acctggtgac cgctcacggt gctgaacact ggttaaagtg
     1081 gatcaatcgc gatacgggtc gtcgctcaga tagtgctcac gcattcgttc atagtaccat
     1141 gcgcaatcat gataatttgt accttatctg taacacgaaa gtcgacaaga ttatcgtgga
     1201 ggacggtcgt gcagccgcgg tacgtaccgt gcctagtaaa ccccttaatc caaagaaacc
     1261 ctcgcacaaa atctatcgcg cacgcaaaca aattgtctta tcgtgtggga cgatttcctc
     1321 tccattagtc cttcagcgct cgggtttcgg ggacccgatt aaattgcgtg cggctggtgt
     1381 taagccctta gttaatcttc ctggagttgg ccgcaacttt caagatcact actgcttctt
     1441 ctctccgtat cgcatcaagc cacaatacga gtctttcgat gactttgtcc gcggtgatgc
     1501 cgaaatccag aagcgtgtat ttgatcagtg gtatgcaaac ggaacgggtc cccttgcaac
     1561 aaacggtatc gaggccggag tgaagattcg tcccactcct gaagagttaa gccagatgga
     1621 cgagagcttc caggaaggct atcgtgaata cttcgaagac aaaccggaca agccggtcat
     1681 gcactactcc atcatcgcag gtttcttcgg cgatcacacc aagattccgc ctgggaaata
     1741 tatgactatg ttccacttcc ttgaatatcc attctctcgt gggtcaattc atattacgtc
     1801 tcccgatcca tatgccgcac ccgactttga ccctggattc atgaacgacg aacgcgacat
     1861 ggcaccgatg gtttgggcat ataagaaatc ccgcgaaact gcacgccgta tggatcattt
     1921 tgctggcgaa gtgaccagcc accatccatt atttccctat tcgtcggagg cccgtgcatt
     1981 ggaaatggat ctggagacgt ccaacgcgta tggagggccc ttaaacttga gcgctggcct
     2041 ggctcatggc tcctggacgc aacctctgaa gaagcccact gcaaagaacg aggggcatgt
     2101 aacctcaaat caggtagaac ttcacccgga catcgaatat gatgaggaag atgataaagc
     2161 catcgagaac tatatccgtg aacataccga gaccacctgg cattgcttag gcacttgcag
     2221 tattgggccg cgtgaaggca gtaaaatcgt gaaatgggga ggggttttgg atcaccgctc
     2281 caacgtctac ggcgttaaag ggctgaaagt cggggattta tctgtatgtc ctgacaacgt
     2341 cggctgtaac acctacacaa cggcattgtt gattggagag aaaacggcta cactggttgg
     2401 ggaagacctt ggttattctg gggaggcgtt ggatatgacg gtcccacaat ttaagttagg
     2461 aacatatgaa aagaccggcc ttgcgcgctt taagcttgcg gccgcactcg agcaccacca
     2521 ccaccaccac tgagatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc
     2581 caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt
     2641 tttgctgaaa ggaggaacta ta
//