BBa_K1991008 1 BBa_K1991008 Pcons-RBS-LO-AOX1-His 2016-10-11T11:00:00Z 2016-10-12T03:17:39Z E. coli (Lpp-OmpA) & Pichia pastoris (AOX) A study showed that Lpp-OmpA (LO) hybrid can direct the heterologous protein GFP to the external surface of E. coli (Enzyme Microb Technol. 2001). Bacillus lipase (J Microbiol. 2014) and Fungi xylanase (Curr Microbiol. 2015) were demonstrated to be displayed on the cell surface of E. coli and maintained the functional enzyme activities. We???d like to display the AOX enzyme on bacterial surface by fusing with Lpp-OmpA and apply to the electrochemical analyzer by depositing on the test strip. false false _2458_ 31417 31417 9 false No false Chen, Pei-En component2493547 1 BBa_B0034 component2493548 1 BBa_K1991005 component2493545 1 BBa_J23101 annotation2493545 1 BBa_J23101 range2493545 1 1 35 annotation2493547 1 BBa_B0034 range2493547 1 44 55 annotation2493548 1 BBa_K1991005 range2493548 1 62 2662 BBa_J23101 1 BBa_J23101 constitutive promoter family member 2006-08-03T11:00:00Z 2015-08-31T04:08:40Z later Released HQ 2013 later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K1991005 1 BBa_K1991005 LO-AOX1-His (Lpp-OmpA-Alcohol Oxidase) 2016-10-11T11:00:00Z 2016-10-12T02:57:28Z E. coli (Lpp-OmpA) & Pichia pastoris (AOX) A study showed that Lpp-OmpA (LO) hybrid can direct the heterologous protein GFP to the external surface of E. coli (Enzyme Microb Technol. 2001). Bacillus lipase (J Microbiol. 2014) and Fungi xylanase (Curr Microbiol. 2015) were demonstrated to be displayed on the cell surface of E. coli and maintained the functional enzyme activities. We???d like to display the AOX enzyme on bacterial surface by fusing with Lpp-OmpA and apply to the electrochemical analyzer by depositing on the test strip. false false _2458_ 31417 31417 9 false No false Chen, Pei-En annotation2521894 1 T7 terminator range2521894 1 2537 2583 annotation2521818 1 Lpp-OmpA range2521818 1 1 441 annotation2521883 1 6X His Tag range2521883 1 2452 2469 annotation2521819 1 AOX1 range2521819 1 442 2430 BBa_B0034_sequence 1 aaagaggagaaa BBa_J23101_sequence 1 tttacagctagctcagtcctaggtattatgctagc BBa_K1991005_sequence 1 atgaaagcaaccaagctggttctgggtgccgtgattctgggcagtaccctgttagcaggttgttctagcaatgccaaaatcgaccaaggcatcaacaacaatggcccgacccacgaaaaccagctgggtgccggtgcctttggtggttatcaggtgaacccgtacgtgggctttgaaatgggctatgattggctgggccgcatgccgtacaaaggcagtgtggagaacggcgcctataaagcacagggcgtgcagctgacagcaaaactgggctaccctattaccgacgacctggacatctacacacgcttaggcggcatggtgtggcgcgccgataccaagagcaacgtgtacggcaagaaccacgataccggcgtgagtccggtgtttgccggcggtgtggagtatgcaatcaccccggaaattgccacacgtggatccatggctattccggaagagttcgatatcttagtgttgggtggaggttcgagcgggtcgtgtattgccggtcgcttggcgaatctggaccacagcttaaaagtaggtcttattgaagcaggtgaaaataacctgaacaacccgtgggtatacttacccggaatttacccacgtaatatgaaacttgacagtaagacggcatcgttctatactagcaatccttctccacatctgaacggtcgccgtaccattgtgccatgcgctaacgtgcttggcggtgggtcaagtattaactttatgatgtatacgcgtggtagtgcttcagactacgatgactttcaagccgaaggttggaaaacgaaagacctgcttcctttaatgaagaaaaccgaaacctaccagcgtgcgtgtaacaatcctgacatccacggattcgaaggaccaatcaaagtgagtttcggcaattacacatatccggtatgtcaggatttcttacgcgcatcggaatcgcaaggtatcccgtatgttgacgatctggaagacctggtgaccgctcacggtgctgaacactggttaaagtggatcaatcgcgatacgggtcgtcgctcagatagtgctcacgcattcgttcatagtaccatgcgcaatcatgataatttgtaccttatctgtaacacgaaagtcgacaagattatcgtggaggacggtcgtgcagccgcggtacgtaccgtgcctagtaaaccccttaatccaaagaaaccctcgcacaaaatctatcgcgcacgcaaacaaattgtcttatcgtgtgggacgatttcctctccattagtccttcagcgctcgggtttcggggacccgattaaattgcgtgcggctggtgttaagcccttagttaatcttcctggagttggccgcaactttcaagatcactactgcttcttctctccgtatcgcatcaagccacaatacgagtctttcgatgactttgtccgcggtgatgccgaaatccagaagcgtgtatttgatcagtggtatgcaaacggaacgggtccccttgcaacaaacggtatcgaggccggagtgaagattcgtcccactcctgaagagttaagccagatggacgagagcttccaggaaggctatcgtgaatacttcgaagacaaaccggacaagccggtcatgcactactccatcatcgcaggtttcttcggcgatcacaccaagattccgcctgggaaatatatgactatgttccacttccttgaatatccattctctcgtgggtcaattcatattacgtctcccgatccatatgccgcacccgactttgaccctggattcatgaacgacgaacgcgacatggcaccgatggtttgggcatataagaaatcccgcgaaactgcacgccgtatggatcattttgctggcgaagtgaccagccaccatccattatttccctattcgtcggaggcccgtgcattggaaatggatctggagacgtccaacgcgtatggagggcccttaaacttgagcgctggcctggctcatggctcctggacgcaacctctgaagaagcccactgcaaagaacgaggggcatgtaacctcaaatcaggtagaacttcacccggacatcgaatatgatgaggaagatgataaagccatcgagaactatatccgtgaacataccgagaccacctggcattgcttaggcacttgcagtattgggccgcgtgaaggcagtaaaatcgtgaaatggggaggggttttggatcaccgctccaacgtctacggcgttaaagggctgaaagtcggggatttatctgtatgtcctgacaacgtcggctgtaacacctacacaacggcattgttgattggagagaaaacggctacactggttggggaagaccttggttattctggggaggcgttggatatgacggtcccacaatttaagttaggaacatatgaaaagaccggccttgcgcgctttaagcttgcggccgcactcgagcaccaccaccaccaccactgagatccggctgctaacaaagcccgaaaggaagctgagttggctgctgccaccgctgagcaataactagcataaccccttggggcctctaaacgggtcttgaggggttttttgctgaaaggaggaactata BBa_K1991008_sequence 1 tttacagctagctcagtcctaggtattatgctagctactagagaaagaggagaaatactagatgaaagcaaccaagctggttctgggtgccgtgattctgggcagtaccctgttagcaggttgttctagcaatgccaaaatcgaccaaggcatcaacaacaatggcccgacccacgaaaaccagctgggtgccggtgcctttggtggttatcaggtgaacccgtacgtgggctttgaaatgggctatgattggctgggccgcatgccgtacaaaggcagtgtggagaacggcgcctataaagcacagggcgtgcagctgacagcaaaactgggctaccctattaccgacgacctggacatctacacacgcttaggcggcatggtgtggcgcgccgataccaagagcaacgtgtacggcaagaaccacgataccggcgtgagtccggtgtttgccggcggtgtggagtatgcaatcaccccggaaattgccacacgtggatccatggctattccggaagagttcgatatcttagtgttgggtggaggttcgagcgggtcgtgtattgccggtcgcttggcgaatctggaccacagcttaaaagtaggtcttattgaagcaggtgaaaataacctgaacaacccgtgggtatacttacccggaatttacccacgtaatatgaaacttgacagtaagacggcatcgttctatactagcaatccttctccacatctgaacggtcgccgtaccattgtgccatgcgctaacgtgcttggcggtgggtcaagtattaactttatgatgtatacgcgtggtagtgcttcagactacgatgactttcaagccgaaggttggaaaacgaaagacctgcttcctttaatgaagaaaaccgaaacctaccagcgtgcgtgtaacaatcctgacatccacggattcgaaggaccaatcaaagtgagtttcggcaattacacatatccggtatgtcaggatttcttacgcgcatcggaatcgcaaggtatcccgtatgttgacgatctggaagacctggtgaccgctcacggtgctgaacactggttaaagtggatcaatcgcgatacgggtcgtcgctcagatagtgctcacgcattcgttcatagtaccatgcgcaatcatgataatttgtaccttatctgtaacacgaaagtcgacaagattatcgtggaggacggtcgtgcagccgcggtacgtaccgtgcctagtaaaccccttaatccaaagaaaccctcgcacaaaatctatcgcgcacgcaaacaaattgtcttatcgtgtgggacgatttcctctccattagtccttcagcgctcgggtttcggggacccgattaaattgcgtgcggctggtgttaagcccttagttaatcttcctggagttggccgcaactttcaagatcactactgcttcttctctccgtatcgcatcaagccacaatacgagtctttcgatgactttgtccgcggtgatgccgaaatccagaagcgtgtatttgatcagtggtatgcaaacggaacgggtccccttgcaacaaacggtatcgaggccggagtgaagattcgtcccactcctgaagagttaagccagatggacgagagcttccaggaaggctatcgtgaatacttcgaagacaaaccggacaagccggtcatgcactactccatcatcgcaggtttcttcggcgatcacaccaagattccgcctgggaaatatatgactatgttccacttccttgaatatccattctctcgtgggtcaattcatattacgtctcccgatccatatgccgcacccgactttgaccctggattcatgaacgacgaacgcgacatggcaccgatggtttgggcatataagaaatcccgcgaaactgcacgccgtatggatcattttgctggcgaagtgaccagccaccatccattatttccctattcgtcggaggcccgtgcattggaaatggatctggagacgtccaacgcgtatggagggcccttaaacttgagcgctggcctggctcatggctcctggacgcaacctctgaagaagcccactgcaaagaacgaggggcatgtaacctcaaatcaggtagaacttcacccggacatcgaatatgatgaggaagatgataaagccatcgagaactatatccgtgaacataccgagaccacctggcattgcttaggcacttgcagtattgggccgcgtgaaggcagtaaaatcgtgaaatggggaggggttttggatcaccgctccaacgtctacggcgttaaagggctgaaagtcggggatttatctgtatgtcctgacaacgtcggctgtaacacctacacaacggcattgttgattggagagaaaacggctacactggttggggaagaccttggttattctggggaggcgttggatatgacggtcccacaatttaagttaggaacatatgaaaagaccggccttgcgcgctttaagcttgcggccgcactcgagcaccaccaccaccaccactgagatccggctgctaacaaagcccgaaaggaagctgagttggctgctgccaccgctgagcaataactagcataaccccttggggcctctaaacgggtcttgaggggttttttgctgaaaggaggaactata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z