BBa_K1991005
1
BBa_K1991005
LO-AOX1-His (Lpp-OmpA-Alcohol Oxidase)
2016-10-11T11:00:00Z
2016-10-12T02:57:28Z
E. coli (Lpp-OmpA) & Pichia pastoris (AOX)
A study showed that Lpp-OmpA (LO) hybrid can direct the heterologous protein GFP to the external surface of E. coli (Enzyme Microb Technol. 2001). Bacillus lipase (J Microbiol. 2014) and Fungi xylanase (Curr Microbiol. 2015) were demonstrated to be displayed on the cell surface of E. coli and maintained the functional enzyme activities. We???d like to display the AOX enzyme on bacterial surface by fusing with Lpp-OmpA and apply to the electrochemical analyzer by depositing on the test strip.
false
false
_2458_
31417
31417
9
false
No
false
Chen, Pei-En
annotation2521894
1
T7 terminator
range2521894
1
2537
2583
annotation2521883
1
6X His Tag
range2521883
1
2452
2469
annotation2521819
1
AOX1
range2521819
1
442
2430
annotation2521818
1
Lpp-OmpA
range2521818
1
1
441
BBa_J23101
1
BBa_J23101
constitutive promoter family member
2006-08-03T11:00:00Z
2015-08-31T04:08:40Z
later
Released HQ 2013
later
false
true
_52_
0
483
95
In stock
true
N/A
true
John Anderson
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_K1991008
1
BBa_K1991008
Pcons-RBS-LO-AOX1-His
2016-10-11T11:00:00Z
2016-10-12T03:17:39Z
E. coli (Lpp-OmpA) & Pichia pastoris (AOX)
A study showed that Lpp-OmpA (LO) hybrid can direct the heterologous protein GFP to the external surface of E. coli (Enzyme Microb Technol. 2001). Bacillus lipase (J Microbiol. 2014) and Fungi xylanase (Curr Microbiol. 2015) were demonstrated to be displayed on the cell surface of E. coli and maintained the functional enzyme activities. We???d like to display the AOX enzyme on bacterial surface by fusing with Lpp-OmpA and apply to the electrochemical analyzer by depositing on the test strip.
false
false
_2458_
31417
31417
9
false
No
false
Chen, Pei-En
component2493548
1
BBa_K1991005
component2493545
1
BBa_J23101
component2493547
1
BBa_B0034
annotation2493548
1
BBa_K1991005
range2493548
1
62
2662
annotation2493545
1
BBa_J23101
range2493545
1
1
35
annotation2493547
1
BBa_B0034
range2493547
1
44
55
BBa_B0034_sequence
1
aaagaggagaaa
BBa_J23101_sequence
1
tttacagctagctcagtcctaggtattatgctagc
BBa_K1991005_sequence
1
atgaaagcaaccaagctggttctgggtgccgtgattctgggcagtaccctgttagcaggttgttctagcaatgccaaaatcgaccaaggcatcaacaacaatggcccgacccacgaaaaccagctgggtgccggtgcctttggtggttatcaggtgaacccgtacgtgggctttgaaatgggctatgattggctgggccgcatgccgtacaaaggcagtgtggagaacggcgcctataaagcacagggcgtgcagctgacagcaaaactgggctaccctattaccgacgacctggacatctacacacgcttaggcggcatggtgtggcgcgccgataccaagagcaacgtgtacggcaagaaccacgataccggcgtgagtccggtgtttgccggcggtgtggagtatgcaatcaccccggaaattgccacacgtggatccatggctattccggaagagttcgatatcttagtgttgggtggaggttcgagcgggtcgtgtattgccggtcgcttggcgaatctggaccacagcttaaaagtaggtcttattgaagcaggtgaaaataacctgaacaacccgtgggtatacttacccggaatttacccacgtaatatgaaacttgacagtaagacggcatcgttctatactagcaatccttctccacatctgaacggtcgccgtaccattgtgccatgcgctaacgtgcttggcggtgggtcaagtattaactttatgatgtatacgcgtggtagtgcttcagactacgatgactttcaagccgaaggttggaaaacgaaagacctgcttcctttaatgaagaaaaccgaaacctaccagcgtgcgtgtaacaatcctgacatccacggattcgaaggaccaatcaaagtgagtttcggcaattacacatatccggtatgtcaggatttcttacgcgcatcggaatcgcaaggtatcccgtatgttgacgatctggaagacctggtgaccgctcacggtgctgaacactggttaaagtggatcaatcgcgatacgggtcgtcgctcagatagtgctcacgcattcgttcatagtaccatgcgcaatcatgataatttgtaccttatctgtaacacgaaagtcgacaagattatcgtggaggacggtcgtgcagccgcggtacgtaccgtgcctagtaaaccccttaatccaaagaaaccctcgcacaaaatctatcgcgcacgcaaacaaattgtcttatcgtgtgggacgatttcctctccattagtccttcagcgctcgggtttcggggacccgattaaattgcgtgcggctggtgttaagcccttagttaatcttcctggagttggccgcaactttcaagatcactactgcttcttctctccgtatcgcatcaagccacaatacgagtctttcgatgactttgtccgcggtgatgccgaaatccagaagcgtgtatttgatcagtggtatgcaaacggaacgggtccccttgcaacaaacggtatcgaggccggagtgaagattcgtcccactcctgaagagttaagccagatggacgagagcttccaggaaggctatcgtgaatacttcgaagacaaaccggacaagccggtcatgcactactccatcatcgcaggtttcttcggcgatcacaccaagattccgcctgggaaatatatgactatgttccacttccttgaatatccattctctcgtgggtcaattcatattacgtctcccgatccatatgccgcacccgactttgaccctggattcatgaacgacgaacgcgacatggcaccgatggtttgggcatataagaaatcccgcgaaactgcacgccgtatggatcattttgctggcgaagtgaccagccaccatccattatttccctattcgtcggaggcccgtgcattggaaatggatctggagacgtccaacgcgtatggagggcccttaaacttgagcgctggcctggctcatggctcctggacgcaacctctgaagaagcccactgcaaagaacgaggggcatgtaacctcaaatcaggtagaacttcacccggacatcgaatatgatgaggaagatgataaagccatcgagaactatatccgtgaacataccgagaccacctggcattgcttaggcacttgcagtattgggccgcgtgaaggcagtaaaatcgtgaaatggggaggggttttggatcaccgctccaacgtctacggcgttaaagggctgaaagtcggggatttatctgtatgtcctgacaacgtcggctgtaacacctacacaacggcattgttgattggagagaaaacggctacactggttggggaagaccttggttattctggggaggcgttggatatgacggtcccacaatttaagttaggaacatatgaaaagaccggccttgcgcgctttaagcttgcggccgcactcgagcaccaccaccaccaccactgagatccggctgctaacaaagcccgaaaggaagctgagttggctgctgccaccgctgagcaataactagcataaccccttggggcctctaaacgggtcttgaggggttttttgctgaaaggaggaactata
BBa_K1991008_sequence
1
tttacagctagctcagtcctaggtattatgctagctactagagaaagaggagaaatactagatgaaagcaaccaagctggttctgggtgccgtgattctgggcagtaccctgttagcaggttgttctagcaatgccaaaatcgaccaaggcatcaacaacaatggcccgacccacgaaaaccagctgggtgccggtgcctttggtggttatcaggtgaacccgtacgtgggctttgaaatgggctatgattggctgggccgcatgccgtacaaaggcagtgtggagaacggcgcctataaagcacagggcgtgcagctgacagcaaaactgggctaccctattaccgacgacctggacatctacacacgcttaggcggcatggtgtggcgcgccgataccaagagcaacgtgtacggcaagaaccacgataccggcgtgagtccggtgtttgccggcggtgtggagtatgcaatcaccccggaaattgccacacgtggatccatggctattccggaagagttcgatatcttagtgttgggtggaggttcgagcgggtcgtgtattgccggtcgcttggcgaatctggaccacagcttaaaagtaggtcttattgaagcaggtgaaaataacctgaacaacccgtgggtatacttacccggaatttacccacgtaatatgaaacttgacagtaagacggcatcgttctatactagcaatccttctccacatctgaacggtcgccgtaccattgtgccatgcgctaacgtgcttggcggtgggtcaagtattaactttatgatgtatacgcgtggtagtgcttcagactacgatgactttcaagccgaaggttggaaaacgaaagacctgcttcctttaatgaagaaaaccgaaacctaccagcgtgcgtgtaacaatcctgacatccacggattcgaaggaccaatcaaagtgagtttcggcaattacacatatccggtatgtcaggatttcttacgcgcatcggaatcgcaaggtatcccgtatgttgacgatctggaagacctggtgaccgctcacggtgctgaacactggttaaagtggatcaatcgcgatacgggtcgtcgctcagatagtgctcacgcattcgttcatagtaccatgcgcaatcatgataatttgtaccttatctgtaacacgaaagtcgacaagattatcgtggaggacggtcgtgcagccgcggtacgtaccgtgcctagtaaaccccttaatccaaagaaaccctcgcacaaaatctatcgcgcacgcaaacaaattgtcttatcgtgtgggacgatttcctctccattagtccttcagcgctcgggtttcggggacccgattaaattgcgtgcggctggtgttaagcccttagttaatcttcctggagttggccgcaactttcaagatcactactgcttcttctctccgtatcgcatcaagccacaatacgagtctttcgatgactttgtccgcggtgatgccgaaatccagaagcgtgtatttgatcagtggtatgcaaacggaacgggtccccttgcaacaaacggtatcgaggccggagtgaagattcgtcccactcctgaagagttaagccagatggacgagagcttccaggaaggctatcgtgaatacttcgaagacaaaccggacaagccggtcatgcactactccatcatcgcaggtttcttcggcgatcacaccaagattccgcctgggaaatatatgactatgttccacttccttgaatatccattctctcgtgggtcaattcatattacgtctcccgatccatatgccgcacccgactttgaccctggattcatgaacgacgaacgcgacatggcaccgatggtttgggcatataagaaatcccgcgaaactgcacgccgtatggatcattttgctggcgaagtgaccagccaccatccattatttccctattcgtcggaggcccgtgcattggaaatggatctggagacgtccaacgcgtatggagggcccttaaacttgagcgctggcctggctcatggctcctggacgcaacctctgaagaagcccactgcaaagaacgaggggcatgtaacctcaaatcaggtagaacttcacccggacatcgaatatgatgaggaagatgataaagccatcgagaactatatccgtgaacataccgagaccacctggcattgcttaggcacttgcagtattgggccgcgtgaaggcagtaaaatcgtgaaatggggaggggttttggatcaccgctccaacgtctacggcgttaaagggctgaaagtcggggatttatctgtatgtcctgacaacgtcggctgtaacacctacacaacggcattgttgattggagagaaaacggctacactggttggggaagaccttggttattctggggaggcgttggatatgacggtcccacaatttaagttaggaacatatgaaaagaccggccttgcgcgctttaagcttgcggccgcactcgagcaccaccaccaccaccactgagatccggctgctaacaaagcccgaaaggaagctgagttggctgctgccaccgctgagcaataactagcataaccccttggggcctctaaacgggtcttgaggggttttttgctgaaaggaggaactata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z