LOCUS       BBa_K1991009            2662 bp    DNA     linear       14-Nov-2024
DEFINITION  Pcons-RBS-LO-AOX2-His
ACCESSION   BBa_K1991009
VERSION     BBa_K1991009.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23101
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             62..502
                     /label=Lpp-OmpA
     CDS             503..2491
                     /label=AOX2
     misc_feature    2598..2644
                     /label=T7 terminator
     misc_feature    2513..2530
                     /label=6X His Tag
     CDS             62..2662
                     /label=BBa_K1991006
ORIGIN
        1 tttacagcta gctcagtcct aggtattatg ctagctacta gagaaagagg agaaatacta
       61 gatgaaagca accaagctgg ttctgggtgc cgtgattctg ggcagtaccc tgttagcagg
      121 ttgttctagc aatgccaaaa tcgaccaagg catcaacaac aatggcccga cccacgaaaa
      181 ccagctgggt gccggtgcct ttggtggtta tcaggtgaac ccgtacgtgg gctttgaaat
      241 gggctatgat tggctgggcc gcatgccgta caaaggcagt gtggagaacg gcgcctataa
      301 agcacagggc gtgcagctga cagcaaaact gggctaccct attaccgacg acctggacat
      361 ctacacacgc ttaggcggca tggtgtggcg cgccgatacc aagagcaacg tgtacggcaa
      421 gaaccacgat accggcgtga gtccggtgtt tgccggcggt gtggagtatg caatcacccc
      481 ggaaattgcc acacgtggat ccatggccat ccctgaggaa tttgacattt tggtcttagg
      541 tggcgggtct tccggctctt gtattgcagg acgcttagcg aacctggatc actcgttaaa
      601 ggttggatta atcgaggctg gcgagaacaa cttaaataac ccgtgggttt atctgccagg
      661 gatctatccg cgtaatatga aattagattc aaaaacggcg tctttctaca cttctaaccc
      721 gtctcctcat ttaaacggac gccgcgctat cgttccatgt gccaatattt tgggaggtgg
      781 gagttctatt aatttcatga tgtatacccg tgggtcggcg tctgattacg acgactttga
      841 ggctgaaggt tggaaaacaa aggacctttt gccgcttatg aagaaaacag aaacttatca
      901 gcgcgcttgt aacaaccccg aaatccacgg ttttgagggc ccaattaaag tttcttttgg
      961 gaattacact tatccagtat gtcaggattt cttacgcgcc acagaatccc agggtattcc
     1021 gtatgtggat gacttagaag accttgtaac cgcacatggt gccgagcact ggttaaaatg
     1081 gattaaccgt gacacaggtc gccgctcgga ttctgctcat gccttcgtac atagcaccat
     1141 gcgtaaccat gataatctgt acctgatttg taataccaag gttgataaaa ttattgtcga
     1201 agacggacgt gcagccgcag tacgtacagt ccctagcaaa ccgctgaatg ccaagaaacc
     1261 aacgcataag gtctatcgtg cgcgcaaaca aattgtctta agctgcggta ctatttctag
     1321 cccgcttgtg ttgcagcgtt ctgggtttgg cgaccccatt aagttgcgtg ctgcgggtgt
     1381 caagcccctt gtgaaccttc ccggcgtcgg gcgcaatttc caggaccact attgcttttt
     1441 cagcccgtac cgcatcaaac cacagtatga gtcgtttgat gatttcgtgc gcggggatgc
     1501 caatatccag aaaaaggtat ttgatcagtg gtatgctaat ggaactggtc ccttggccac
     1561 aaacggtatc gaggcagggg taaagatccg ccctacccca gaggagttaa gccaaatgga
     1621 tgaatcattc caggaaggtt accgcgagta ttttgaagac aagccggata agccggttat
     1681 gcactatagt attatcgccg gtttcttcgg cgatcatacc aaaattccgc ctggaaagta
     1741 tatgacgatg tttcatttct tggagtaccc tttctcccgc gggtcgatcc acatcacgtc
     1801 cccggacccc tatgcgaccc cggattttga tccggggttc atgaacgatg aacgcgatat
     1861 ggcacctatg gtgtggtcat acaagaaatc acgtgagacg gcacgcaaga tggaccattt
     1921 cgctggcgag gtgacgtccc atcatcctct tttcccatac agttcggagg cccgtgcgta
     1981 cgagatggat cttgaaacga gtaatgcgta cggaggtccg ctgaacttaa ctgccgggtt
     2041 agcgcatggc tcgtggacgc aaccattaaa gaaaccggca ggacgcaatg aggggcacgt
     2101 gacgtcaaac caggtggaat tacatccaga cattgaatac gacgaggaag acgataaagc
     2161 gattgagaac tacatccgtg agcacacaga aactacttgg cactgtttgg gtacttgctc
     2221 gatcggtccg cgtgaaggct caaagatcgt taaatggggt ggcgtcttgg atcatcgtag
     2281 taatgtatac ggtgtgaaag gtcttaaggt aggtgatctt tccgtgtgcc cagacaacgt
     2341 tggctgtaat acttacacta cagctctgtt aatcggtgag aagactgcga cattggttgg
     2401 tgaggatctt ggctataccg gcgaggcatt agacatgacc gttcctcaat ttaaattggg
     2461 aacttacgag aagacgggct tagcgcgctt caagcttgcg gccgcactcg agcaccacca
     2521 ccaccaccac tgagatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc
     2581 caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt
     2641 tttgctgaaa ggaggaacta ta
//