LOCUS       BBa_K1991010            1384 bp    DNA     linear       22-Nov-2024
DEFINITION  Pcons-RBS-LO-GFP-His
ACCESSION   BBa_K1991010
VERSION     BBa_K1991010.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23101
     RBS             44..55
                     /label=BBa_B0034
     CDS             62..496
                     /label=Lpp-OmpA
     CDS             503..1213
                     /label=GFP
     misc_feature    1235..1252
                     /label=6X His Tag
     misc_feature    1320..1366
                     /label=T7 terminator
ORIGIN
        1 tttacagcta gctcagtcct aggtattatg ctagctacta gagaaagagg agaaatacta
       61 gatgaaagca accaagctgg ttctgggtgc cgtgattctg ggcagtaccc tgttagcagg
      121 ttgttctagc aatgccaaaa tcgaccaagg catcaacaac aatggcccga cccacgaaaa
      181 ccagctgggt gccggtgcct ttggtggtta tcaggtgaac ccgtacgtgg gctttgaaat
      241 gggctatgat tggctgggcc gcatgccgta caaaggcagt gtggagaacg gcgcctataa
      301 agcacagggc gtgcagctga cagcaaaact gggctaccct attaccgacg acctggacat
      361 ctacacacgc ttaggcggca tggtgtggcg cgccgatacc aagagcaacg tgtacggcaa
      421 gaaccacgat accggcgtga gtccggtgtt tgccggcggt gtggagtatg caatcacccc
      481 ggaaattgcc acacgtggat cccgtaaagg agaagaactt ttcactggag ttgtcccaat
      541 tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga
      601 aggtgatgca acatacggaa aacttaccct taaatttatt tgcactactg gaaaactacc
      661 tgttccatgg ccaacacttg tcactacttt cggttatggt gttcaatgct ttgcgagata
      721 cccagatcat atgaaacagc atgacttttt caagagtgcc atgcccgaag gttatgtaca
      781 ggaaagaact atatttttca aagatgacgg gaactacaag acacgtgctg aagtcaagtt
      841 tgaaggtgat acccttgtta atagaatcga gttaaaaggt attgatttta aagaagatgg
      901 aaacattctt ggacacaaat tggaatacaa ctataactca cacaatgtat acatcatggc
      961 agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg
     1021 aagcgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg gccctgtcct
     1081 tttaccagac aaccattacc tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa
     1141 gagagaccac atggtccttc ttgagtttgt aacagctgct gggattacac atggcatgga
     1201 tgaactatac aaaaagcttg cggccgcact cgagcaccac caccaccacc actgagatcc
     1261 ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact
     1321 agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac
     1381 tata
//