LOCUS       BBa_K199104             2627 bp    DNA     linear       22-Nov-2024
DEFINITION  pLac-RBS-LuxR-TT-RFP-RBS-pOmpC-pLux-RBS-GFP
ACCESSION   BBa_K199104
VERSION     BBa_K199104.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     misc_feature    82..83
                     /label=prefix
     misc_feature    255..255
                     /label=T
     misc_feature    573..573
                     /label=A
     CDS             82..831
                     /label=luxR
     misc_feature    82..837
                     /label=BBa_C0062
     misc_feature    838..862
                     /label=Help:Barcodes
     CDS             82..837
                     /label=BBa_C0062
     stem_loop       882..925
                     /label=stem_loop
     misc_feature    871..950
                     /label=BBa_B0010
     terminator      871..950
                     /label=BBa_B0010
     stem_loop       966..985
                     /label=T7 TE
     misc_feature    992..992
                     /label=stop
     polyA_site      986..999
                     /label=polya
     misc_feature    959..999
                     /label=BBa_B0012
     terminator      959..999
                     /label=BBa_B0012
     misc_feature    64..999
                     /label=BBa_I0462
     misc_feature    1..999
                     /label=BBa_K199103
     CDS             1008..1685
                     /label=mRFP reverse
     primer_bind     1666..1685
                     /label=Spe mRFP F
     primer_bind     1008..1027
                     /label=Xba mRFP R
     misc_feature    1008..1685
                     /label=BBa_J31008
     RBS             1694..1708
                     /label=Reverse RBS
     RBS             1694..1708
                     /label=BBa_J44001
     promoter        1717..1815
                     /label=BBa_K199017
     promoter        1824..1878
                     /label=BBa_K199052
     RBS             1887..1901
                     /label=RBS-1\Strong
     RBS             1894..1898
                     /label=RBS
     misc_feature    1887..1901
                     /label=BBa_B0030
     RBS             1887..1901
                     /label=BBa_B0030
     CDS             1908..2627
                     /label=GFP protein
     CDS             1908..2627
                     /label=BBa_E0040
     misc_feature    1008..2627
                     /label=BBa_K199069
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatgaaaaac ataaatgccg acgacacata cagaataatt
      121 aataaaatta aagcttgtag aagcaataat gatattaatc aatgcttatc tgatatgact
      181 aaaatggtac attgtgaata ttatttactc gcgatcattt atcctcattc tatggttaaa
      241 tctgatattt caatcctaga taattaccct aaaaaatgga ggcaatatta tgatgacgct
      301 aatttaataa aatatgatcc tatagtagat tattctaact ccaatcattc accaattaat
      361 tggaatatat ttgaaaacaa tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg
      421 aaaacatcag gtcttatcac tgggtttagt ttccctattc atacggctaa caatggcttc
      481 ggaatgctta gttttgcaca ttcagaaaaa gacaactata tagatagttt atttttacat
      541 gcgtgtatga acataccatt aattgttcct tctctagttg ataattatcg aaaaataaat
      601 atagcaaata ataaatcaaa caacgattta accaaaagag aaaaagaatg tttagcgtgg
      661 gcatgcgaag gaaaaagctc ttgggatatt tcaaaaatat taggttgcag tgagcgtact
      721 gtcactttcc atttaaccaa tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt
      781 atttctaaag caattttaac aggagcaatt gattgcccat actttaaaaa ttaataacac
      841 tgatagtgct agtgtagatc actactagag ccaggcatca aataaaacga aaggctcagt
      901 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc
      961 acactggctc accttcgggt gggcctttct gcgtttatat actagagtta agcaccggtg
     1021 gagtgacgac cttcagcacg ttcgtactgt tcaacgatgg tgtagtcttc gttgtgggag
     1081 gtgatgtcca gtttgatgtc ggttttgtaa gcacccggca gctgaaccgg ttttttagcc
     1141 atgtaggtgg ttttaacttc agcgtcgtag tgaccaccgt ctttcagttt cagacgcatt
     1201 ttgatttcac ctttcagagc accgtcttcc gggtacatac gttcggtgga agcttcccaa
     1261 cccatggttt ttttctgcat aaccggaccg tcggacggga agttggtacc acgcagttta
     1321 actttgtaga tgaactcacc gtcttgcagg gaggagtcct gggtaacggt aacaacacca
     1381 ccgtcttcga agttcataac acgttcccat ttgaaacctt ccgggaagga cagtttcagg
     1441 tagtccggga tgtcagccgg gtgtttaacg taagctttgg aaccgtactg gaactgcggg
     1501 gacaggatgt cccaagcgaa cggcagcgga ccacctttgg taactttcag tttagcggtc
     1561 tgggtacctt cgtacggacg accttcacct tcaccttcga tttcgaactc gtgaccgtta
     1621 acggaacctt ccatacgaac tttgaaacgc atgaactctt tgataacgtc ttcggaggaa
     1681 gccattacta gagtttctcc tctttaatta ctagagttta cattttgaaa catctatagc
     1741 gataaatgaa acatcttaaa agttttagta tcatattcgt gttggattat tctgcatttt
     1801 tggggagaat ggacttacta gagtttattc gactataaca aaccattttc ttgcgtaaac
     1861 ctgtacgatc ctacaggtta ctagagatta aagaggagaa atactagatg cgtaaaggag
     1921 aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat gttaatgggc
     1981 acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa cttaccctta
     2041 aatttatttg cactactgga aaactacctg ttccatggcc aacacttgtc actactttcg
     2101 gttatggtgt tcaatgcttt gcgagatacc cagatcatat gaaacagcat gactttttca
     2161 agagtgccat gcccgaaggt tatgtacagg aaagaactat atttttcaaa gatgacggga
     2221 actacaagac acgtgctgaa gtcaagtttg aaggtgatac ccttgttaat agaatcgagt
     2281 taaaaggtat tgattttaaa gaagatggaa acattcttgg acacaaattg gaatacaact
     2341 ataactcaca caatgtatac atcatggcag acaaacaaaa gaatggaatc aaagttaact
     2401 tcaaaattag acacaacatt gaagatggaa gcgttcaact agcagaccat tatcaacaaa
     2461 atactccaat tggcgatggc cctgtccttt taccagacaa ccattacctg tccacacaat
     2521 ctgccctttc gaaagatccc aacgaaaaga gagaccacat ggtccttctt gagtttgtaa
     2581 cagctgctgg gattacacat ggcatggatg aactatacaa ataataa
//