##gff-version 3
##sequence-region BBa_K199104 1 2627
BBa_K199104	.	engineered_region	1	999	.	+	0	ID=BBa_K199103;Name=BBa_K199103
BBa_K199104	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_K199103
BBa_K199104	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_K199104	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_K199104	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_K199104	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_K199104	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_K199104	.	engineered_region	64	999	.	+	0	ID=BBa_I0462;Name=BBa_I0462;Parent=BBa_K199103
BBa_K199104	.	CDS	82	837	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_I0462
BBa_K199104	.	CDS	82	831	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K199104	.	engineered_region	82	837	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K199104	.	engineered_region	82	83	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K199104	.	point_mutation	573	573	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K199104	.	engineered_tag	838	862	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K199104	.	point_mutation	255	255	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K199104	.	terminator	959	999	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0462
BBa_K199104	.	engineered_region	959	999	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K199104	.	stem_loop	966	985	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K199104	.	stop_codon	992	992	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K199104	.	polyA_site	986	999	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K199104	.	terminator	871	950	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0462
BBa_K199104	.	engineered_region	871	950	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K199104	.	stem_loop	882	925	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K199104	.	ribosome_entry_site	64	75	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0462
BBa_K199104	.	conserved_region	68	71	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K199104	.	engineered_region	1008	2627	.	+	0	ID=BBa_K199069;Name=BBa_K199069
BBa_K199104	.	promoter	1824	1878	.	+	0	ID=BBa_K199052;Name=BBa_K199052;Parent=BBa_K199069
BBa_K199104	.	engineered_region	1008	1685	.	+	0	ID=BBa_J31008;Name=BBa_J31008;Parent=BBa_K199069
BBa_K199104	.	primer_binding_site	1666	1685	.	+	0	ID=annotation1911764;Name=Spe mRFP F;Parent=BBa_J31008
BBa_K199104	.	primer_binding_site	1008	1027	.	+	0	ID=annotation1911765;Name=Xba mRFP R;Parent=BBa_J31008
BBa_K199104	.	CDS	1008	1685	.	+	0	ID=annotation1911761;Name=mRFP reverse;Parent=BBa_J31008
BBa_K199104	.	promoter	1717	1815	.	+	0	ID=BBa_K199017;Name=BBa_K199017;Parent=BBa_K199069
BBa_K199104	.	ribosome_entry_site	1887	1901	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K199069
BBa_K199104	.	ribosome_entry_site	1894	1898	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K199104	.	engineered_region	1887	1901	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K199104	.	ribosome_entry_site	1887	1901	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K199104	.	ribosome_entry_site	1694	1708	.	+	0	ID=BBa_J44001;Name=BBa_J44001;Parent=BBa_K199069
BBa_K199104	.	ribosome_entry_site	1694	1708	.	+	0	ID=annotation1893199;Name=Reverse RBS;Parent=BBa_J44001
BBa_K199104	.	CDS	1908	2627	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_K199069
BBa_K199104	.	CDS	1908	2627	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
>BBa_K199104
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatact
agatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaa
tcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggtt
aaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatg
atcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaa
atctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaat
ggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaaca
taccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaac
caaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagt
gagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaag
caattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactaga
gccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctc
tctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttaagcaccggtggagtgac
gaccttcagcacgttcgtactgttcaacgatggtgtagtcttcgttgtgggaggtgatgtccagtttgatgtcggtttt
gtaagcacccggcagctgaaccggttttttagccatgtaggtggttttaacttcagcgtcgtagtgaccaccgtctttc
agtttcagacgcattttgatttcacctttcagagcaccgtcttccgggtacatacgttcggtggaagcttcccaaccca
tggtttttttctgcataaccggaccgtcggacgggaagttggtaccacgcagtttaactttgtagatgaactcaccgtc
ttgcagggaggagtcctgggtaacggtaacaacaccaccgtcttcgaagttcataacacgttcccatttgaaaccttcc
gggaaggacagtttcaggtagtccgggatgtcagccgggtgtttaacgtaagctttggaaccgtactggaactgcgggg
acaggatgtcccaagcgaacggcagcggaccacctttggtaactttcagtttagcggtctgggtaccttcgtacggacg
accttcaccttcaccttcgatttcgaactcgtgaccgttaacggaaccttccatacgaactttgaaacgcatgaactct
ttgataacgtcttcggaggaagccattactagagtttctcctctttaattactagagtttacattttgaaacatctata
gcgataaatgaaacatcttaaaagttttagtatcatattcgtgttggattattctgcatttttggggagaatggactta
ctagagtttattcgactataacaaaccattttcttgcgtaaacctgtacgatcctacaggttactagagattaaagagg
agaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaa
tgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcact
actggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccag
atcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaaga
tgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtatt
gattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcag
acaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagacca
ttatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctt
tcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatgg
atgaactatacaaataataa