LOCUS       BBa_K199107             1145 bp    DNA     linear       27-Dec-2024
DEFINITION  pLpp-CCACU tRNA-pLac-RBS-CCACU-RFP
ACCESSION   BBa_K199107
VERSION     BBa_K199107.1  
FEATURES             Location/Qualifiers
     promoter        1..49
                     /label=BBa_K199106
     misc_feature    58..68
                     /label=5' Context
     misc_feature    101..109
                     /label=Anticodon Loop
     misc_feature    161..200
                     /label=3' Context
     misc_feature    58..200
                     /label=BBa_K199002
     CDS             209..296
                     /label=end of LacI coding region (inactive)
     misc_feature    209..408
                     /label=BBa_R0010
     misc_feature    297..334
                     /label=CAP binding site
     promoter        345..350
                     /label=-35
     promoter        369..374
                     /label=-10
     misc_feature    374..408
                     /label=LacI binding site
     misc_feature    381..381
                     /label=start
     promoter        209..408
                     /label=BBa_R0010
     misc_feature    421..424
                     /label=conserved
     RBS             417..428
                     /label=BBa_B0034
     misc_feature    438..442
                     /label=5 base pair addition
     misc_feature    858..863
                     /label=Nco1 restriction site
     misc_feature    1121..1145
                     /label=25 bp addition
     misc_feature    1115..1120
                     /label=Double Stop
     misc_feature    435..1145
                     /label=BBa_K199005
ORIGIN
        1 atcaaaaaaa tattctcaac ataaaaaact ttgtgtaata cttgtaacgt actagaggga
       61 tccaattcgg agagatgccg gagcggctga acggaccggt ctagtggaca ccggagtagg
      121 ggcaactcta ccgggggttc aaatccccct ctctccgcca ctgcatatcc ttagcgaaag
      181 ctaaggattt tttttaagct tactagagca atacgcaaac cgcctctccc cgcgcgttgg
      241 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
      301 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
      361 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacata ctagagaaag
      421 aggagaaata ctagatgcca ctgcttcctc cgaagacgtt atcaaagagt tcatgcgttt
      481 caaagttcgt atggaaggtt ccgttaacgg tcacgagttc gaaatcgaag gtgaaggtga
      541 aggtcgtccg tacgaaggta cccagaccgc taaactgaaa gttaccaaag gtggtccgct
      601 gccgttcgct tgggacatcc tgtccccgca gttccagtac ggttccaaag cttacgttaa
      661 acacccggct gacatcccgg actacctgaa actgtccttc ccggaaggtt tcaaatggga
      721 acgtgttatg aacttcgaag acggtggtgt tgttaccgtt acccaggact cctccctgca
      781 agacggtgag ttcatctaca aagttaaact gcgtggtacc aacttcccgt ccgacggtcc
      841 ggttatgcag aaaaaaacca tgggttggga agcttccacc gaacgtatgt acccggaaga
      901 cggtgctctg aaaggtgaaa tcaaaatgcg tctgaaactg aaagacggtg gtcactacga
      961 cgctgaagtt aaaaccacct acatggctaa aaaaccggtt cagctgccgg gtgcttacaa
     1021 aaccgacatc aaactggaca tcacctccca caacgaagac tacaccatcg ttgaacagta
     1081 cgaacgtgct gaaggtcgtc actccaccgg tgcttaataa cgctgatagt gctagtgtag
     1141 atcgc
//