LOCUS       BBa_K199113             1995 bp    DNA     linear       27-Dec-2024
DEFINITION  RFP-TT-pLac-RBS-LuxR-TT
ACCESSION   BBa_K199113
VERSION     BBa_K199113.1  
FEATURES             Location/Qualifiers
     CDS             1..675
                     /label=mrfp1
     misc_feature    682..706
                     /label=Help:Barcodes
     CDS             1..681
                     /label=BBa_E1010
     stem_loop       726..769
                     /label=stem_loop
     misc_feature    715..794
                     /label=BBa_B0010
     terminator      715..794
                     /label=BBa_B0010
     stem_loop       810..829
                     /label=T7 TE
     misc_feature    836..836
                     /label=stop
     polyA_site      830..843
                     /label=polya
     misc_feature    803..843
                     /label=BBa_B0012
     terminator      803..843
                     /label=BBa_B0012
     misc_feature    1..843
                     /label=BBa_I13501
     CDS             852..939
                     /label=end of LacI coding region (inactive)
     misc_feature    852..1051
                     /label=BBa_R0010
     misc_feature    940..977
                     /label=CAP binding site
     promoter        988..993
                     /label=-35
     promoter        1012..1017
                     /label=-10
     misc_feature    1017..1051
                     /label=LacI binding site
     misc_feature    1024..1024
                     /label=start
     promoter        852..1051
                     /label=BBa_R0010
     misc_feature    1064..1067
                     /label=conserved
     RBS             1060..1071
                     /label=BBa_B0034
     misc_feature    1078..1079
                     /label=prefix
     misc_feature    1251..1251
                     /label=T
     misc_feature    1569..1569
                     /label=A
     CDS             1078..1827
                     /label=luxR
     misc_feature    1078..1833
                     /label=BBa_C0062
     misc_feature    1834..1858
                     /label=Help:Barcodes
     CDS             1078..1833
                     /label=BBa_C0062
     stem_loop       1878..1921
                     /label=stem_loop
     misc_feature    1867..1946
                     /label=BBa_B0010
     terminator      1867..1946
                     /label=BBa_B0010
     stem_loop       1962..1981
                     /label=T7 TE
     misc_feature    1988..1988
                     /label=stop
     polyA_site      1982..1995
                     /label=polya
     misc_feature    1955..1995
                     /label=BBa_B0012
     terminator      1955..1995
                     /label=BBa_B0012
     misc_feature    1060..1995
                     /label=BBa_I0462
     misc_feature    852..1995
                     /label=BBa_K109702
ORIGIN
        1 atggcttcct ccgaagacgt tatcaaagag ttcatgcgtt tcaaagttcg tatggaaggt
       61 tccgttaacg gtcacgagtt cgaaatcgaa ggtgaaggtg aaggtcgtcc gtacgaaggt
      121 acccagaccg ctaaactgaa agttaccaaa ggtggtccgc tgccgttcgc ttgggacatc
      181 ctgtccccgc agttccagta cggttccaaa gcttacgtta aacacccggc tgacatcccg
      241 gactacctga aactgtcctt cccggaaggt ttcaaatggg aacgtgttat gaacttcgaa
      301 gacggtggtg ttgttaccgt tacccaggac tcctccctgc aagacggtga gttcatctac
      361 aaagttaaac tgcgtggtac caacttcccg tccgacggtc cggttatgca gaaaaaaacc
      421 atgggttggg aagcttccac cgaacgtatg tacccggaag acggtgctct gaaaggtgaa
      481 atcaaaatgc gtctgaaact gaaagacggt ggtcactacg acgctgaagt taaaaccacc
      541 tacatggcta aaaaaccggt tcagctgccg ggtgcttaca aaaccgacat caaactggac
      601 atcacctccc acaacgaaga ctacaccatc gttgaacagt acgaacgtgc tgaaggtcgt
      661 cactccaccg gtgcttaata acgctgatag tgctagtgta gatcgctact agagccaggc
      721 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
      781 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
      841 atatactaga gcaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
      901 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      961 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     1021 tggaattgtg agcggataac aatttcacac atactagaga aagaggagaa atactagatg
     1081 aaaaacataa atgccgacga cacatacaga ataattaata aaattaaagc ttgtagaagc
     1141 aataatgata ttaatcaatg cttatctgat atgactaaaa tggtacattg tgaatattat
     1201 ttactcgcga tcatttatcc tcattctatg gttaaatctg atatttcaat cctagataat
     1261 taccctaaaa aatggaggca atattatgat gacgctaatt taataaaata tgatcctata
     1321 gtagattatt ctaactccaa tcattcacca attaattgga atatatttga aaacaatgct
     1381 gtaaataaaa aatctccaaa tgtaattaaa gaagcgaaaa catcaggtct tatcactggg
     1441 tttagtttcc ctattcatac ggctaacaat ggcttcggaa tgcttagttt tgcacattca
     1501 gaaaaagaca actatataga tagtttattt ttacatgcgt gtatgaacat accattaatt
     1561 gttccttctc tagttgataa ttatcgaaaa ataaatatag caaataataa atcaaacaac
     1621 gatttaacca aaagagaaaa agaatgttta gcgtgggcat gcgaaggaaa aagctcttgg
     1681 gatatttcaa aaatattagg ttgcagtgag cgtactgtca ctttccattt aaccaatgcg
     1741 caaatgaaac tcaatacaac aaaccgctgc caaagtattt ctaaagcaat tttaacagga
     1801 gcaattgatt gcccatactt taaaaattaa taacactgat agtgctagtg tagatcacta
     1861 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
     1921 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc
     1981 ctttctgcgt ttata
//