##gff-version 3
##sequence-region BBa_K199113 1 1995
BBa_K199113	.	engineered_region	852	1995	.	+	0	ID=BBa_K109702;Name=BBa_K109702
BBa_K199113	.	promoter	852	1051	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_K109702
BBa_K199113	.	non_covalent_binding_site	1017	1051	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K199113	.	non_covalent_binding_site	940	977	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K199113	.	promoter	988	993	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K199113	.	start_codon	1024	1024	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K199113	.	engineered_region	852	1051	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K199113	.	CDS	852	939	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K199113	.	promoter	1012	1017	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K199113	.	engineered_region	1060	1995	.	+	0	ID=BBa_I0462;Name=BBa_I0462;Parent=BBa_K109702
BBa_K199113	.	terminator	1955	1995	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0462
BBa_K199113	.	polyA_site	1982	1995	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K199113	.	stop_codon	1988	1988	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K199113	.	stem_loop	1962	1981	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K199113	.	engineered_region	1955	1995	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K199113	.	CDS	1078	1833	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_I0462
BBa_K199113	.	engineered_region	1078	1833	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K199113	.	engineered_tag	1834	1858	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K199113	.	engineered_region	1078	1079	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K199113	.	point_mutation	1251	1251	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K199113	.	point_mutation	1569	1569	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K199113	.	CDS	1078	1827	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K199113	.	terminator	1867	1946	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0462
BBa_K199113	.	stem_loop	1878	1921	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K199113	.	engineered_region	1867	1946	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K199113	.	ribosome_entry_site	1060	1071	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0462
BBa_K199113	.	conserved_region	1064	1067	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K199113	.	engineered_region	1	843	.	+	0	ID=BBa_I13501;Name=BBa_I13501
BBa_K199113	.	terminator	715	794	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13501
BBa_K199113	.	stem_loop	726	769	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K199113	.	engineered_region	715	794	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K199113	.	CDS	1	681	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_I13501
BBa_K199113	.	CDS	1	675	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K199113	.	engineered_tag	682	706	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K199113	.	terminator	803	843	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13501
BBa_K199113	.	polyA_site	830	843	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K199113	.	stop_codon	836	836	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K199113	.	stem_loop	810	829	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K199113	.	engineered_region	803	843	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
>BBa_K199113
atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagt
tcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtcc
gctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatc
ccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgtta
ccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccga
cggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaa
ggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaa
aaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgt
tgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctacta
gagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgc
tctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcaatacgcaaaccgcctc
tccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgc
aattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaatt
gtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacata
cagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggta
cattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattacc
ctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatca
ttcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaaca
tcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcag
aaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataa
ttatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggca
tgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatg
cgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccata
ctttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagt
cgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcggg
tgggcctttctgcgtttata