LOCUS       BBa_K199116             2013 bp    DNA     linear       29-Nov-2024
DEFINITION  RBS-RFP-TT-pLac-RBS-LuxR-TT
ACCESSION   BBa_K199116
VERSION     BBa_K199116.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..693
                     /label=mrfp1
     misc_feature    700..724
                     /label=Help:Barcodes
     CDS             19..699
                     /label=BBa_E1010
     stem_loop       744..787
                     /label=stem_loop
     misc_feature    733..812
                     /label=BBa_B0010
     terminator      733..812
                     /label=BBa_B0010
     stem_loop       828..847
                     /label=T7 TE
     misc_feature    854..854
                     /label=stop
     polyA_site      848..861
                     /label=polya
     misc_feature    821..861
                     /label=BBa_B0012
     terminator      821..861
                     /label=BBa_B0012
     misc_feature    19..861
                     /label=BBa_I13501
     CDS             870..957
                     /label=end of LacI coding region (inactive)
     misc_feature    870..1069
                     /label=BBa_R0010
     misc_feature    958..995
                     /label=CAP binding site
     promoter        1006..1011
                     /label=-35
     promoter        1030..1035
                     /label=-10
     misc_feature    1035..1069
                     /label=LacI binding site
     misc_feature    1042..1042
                     /label=start
     promoter        870..1069
                     /label=BBa_R0010
     misc_feature    1082..1085
                     /label=conserved
     RBS             1078..1089
                     /label=BBa_B0034
     misc_feature    1096..1097
                     /label=prefix
     misc_feature    1269..1269
                     /label=T
     misc_feature    1587..1587
                     /label=A
     CDS             1096..1845
                     /label=luxR
     misc_feature    1096..1851
                     /label=BBa_C0062
     misc_feature    1852..1876
                     /label=Help:Barcodes
     CDS             1096..1851
                     /label=BBa_C0062
     stem_loop       1896..1939
                     /label=stem_loop
     misc_feature    1885..1964
                     /label=BBa_B0010
     terminator      1885..1964
                     /label=BBa_B0010
     stem_loop       1980..1999
                     /label=T7 TE
     misc_feature    2006..2006
                     /label=stop
     polyA_site      2000..2013
                     /label=polya
     misc_feature    1973..2013
                     /label=BBa_B0012
     terminator      1973..2013
                     /label=BBa_B0012
     misc_feature    1078..2013
                     /label=BBa_I0462
     misc_feature    870..2013
                     /label=BBa_K109702
     misc_feature    19..2013
                     /label=BBa_K199113
ORIGIN
        1 aaagaggaga aatactagat ggcttcctcc gaagacgtta tcaaagagtt catgcgtttc
       61 aaagttcgta tggaaggttc cgttaacggt cacgagttcg aaatcgaagg tgaaggtgaa
      121 ggtcgtccgt acgaaggtac ccagaccgct aaactgaaag ttaccaaagg tggtccgctg
      181 ccgttcgctt gggacatcct gtccccgcag ttccagtacg gttccaaagc ttacgttaaa
      241 cacccggctg acatcccgga ctacctgaaa ctgtccttcc cggaaggttt caaatgggaa
      301 cgtgttatga acttcgaaga cggtggtgtt gttaccgtta cccaggactc ctccctgcaa
      361 gacggtgagt tcatctacaa agttaaactg cgtggtacca acttcccgtc cgacggtccg
      421 gttatgcaga aaaaaaccat gggttgggaa gcttccaccg aacgtatgta cccggaagac
      481 ggtgctctga aaggtgaaat caaaatgcgt ctgaaactga aagacggtgg tcactacgac
      541 gctgaagtta aaaccaccta catggctaaa aaaccggttc agctgccggg tgcttacaaa
      601 accgacatca aactggacat cacctcccac aacgaagact acaccatcgt tgaacagtac
      661 gaacgtgctg aaggtcgtca ctccaccggt gcttaataac gctgatagtg ctagtgtaga
      721 tcgctactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
      781 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg
      841 gtgggccttt ctgcgtttat atactagagc aatacgcaaa ccgcctctcc ccgcgcgttg
      901 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
      961 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
     1021 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacat actagagaaa
     1081 gaggagaaat actagatgaa aaacataaat gccgacgaca catacagaat aattaataaa
     1141 attaaagctt gtagaagcaa taatgatatt aatcaatgct tatctgatat gactaaaatg
     1201 gtacattgtg aatattattt actcgcgatc atttatcctc attctatggt taaatctgat
     1261 atttcaatcc tagataatta ccctaaaaaa tggaggcaat attatgatga cgctaattta
     1321 ataaaatatg atcctatagt agattattct aactccaatc attcaccaat taattggaat
     1381 atatttgaaa acaatgctgt aaataaaaaa tctccaaatg taattaaaga agcgaaaaca
     1441 tcaggtctta tcactgggtt tagtttccct attcatacgg ctaacaatgg cttcggaatg
     1501 cttagttttg cacattcaga aaaagacaac tatatagata gtttattttt acatgcgtgt
     1561 atgaacatac cattaattgt tccttctcta gttgataatt atcgaaaaat aaatatagca
     1621 aataataaat caaacaacga tttaaccaaa agagaaaaag aatgtttagc gtgggcatgc
     1681 gaaggaaaaa gctcttggga tatttcaaaa atattaggtt gcagtgagcg tactgtcact
     1741 ttccatttaa ccaatgcgca aatgaaactc aatacaacaa accgctgcca aagtatttct
     1801 aaagcaattt taacaggagc aattgattgc ccatacttta aaaattaata acactgatag
     1861 tgctagtgta gatcactact agagccaggc atcaaataaa acgaaaggct cagtcgaaag
     1921 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg
     1981 gctcaccttc gggtgggcct ttctgcgttt ata
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