##gff-version 3
##sequence-region BBa_K199116 1 2013
BBa_K199116	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K199116	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K199116	.	engineered_region	19	2013	.	+	0	ID=BBa_K199113;Name=BBa_K199113
BBa_K199116	.	engineered_region	870	2013	.	+	0	ID=BBa_K109702;Name=BBa_K109702;Parent=BBa_K199113
BBa_K199116	.	engineered_region	1078	2013	.	+	0	ID=BBa_I0462;Name=BBa_I0462;Parent=BBa_K109702
BBa_K199116	.	terminator	1973	2013	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0462
BBa_K199116	.	stop_codon	2006	2006	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K199116	.	polyA_site	2000	2013	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K199116	.	stem_loop	1980	1999	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K199116	.	engineered_region	1973	2013	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K199116	.	CDS	1096	1851	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_I0462
BBa_K199116	.	engineered_tag	1852	1876	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K199116	.	engineered_region	1096	1851	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K199116	.	engineered_region	1096	1097	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K199116	.	point_mutation	1269	1269	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K199116	.	CDS	1096	1845	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K199116	.	point_mutation	1587	1587	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K199116	.	terminator	1885	1964	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0462
BBa_K199116	.	engineered_region	1885	1964	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K199116	.	stem_loop	1896	1939	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K199116	.	ribosome_entry_site	1078	1089	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0462
BBa_K199116	.	conserved_region	1082	1085	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K199116	.	promoter	870	1069	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_K109702
BBa_K199116	.	non_covalent_binding_site	958	995	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K199116	.	non_covalent_binding_site	1035	1069	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K199116	.	promoter	1006	1011	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K199116	.	engineered_region	870	1069	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K199116	.	promoter	1030	1035	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K199116	.	start_codon	1042	1042	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K199116	.	CDS	870	957	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K199116	.	engineered_region	19	861	.	+	0	ID=BBa_I13501;Name=BBa_I13501;Parent=BBa_K199113
BBa_K199116	.	CDS	19	699	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_I13501
BBa_K199116	.	engineered_tag	700	724	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K199116	.	CDS	19	693	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K199116	.	terminator	733	812	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13501
BBa_K199116	.	engineered_region	733	812	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K199116	.	stem_loop	744	787	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K199116	.	terminator	821	861	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13501
BBa_K199116	.	stop_codon	854	854	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K199116	.	polyA_site	848	861	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K199116	.	stem_loop	828	847	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K199116	.	engineered_region	821	861	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
>BBa_K199116
aaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggtt
ccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaa
agttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgtt
aaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcg
aagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtgg
taccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccg
gaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaa
ccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaa
cgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgc
tagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctg
ttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagag
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgaaaaacat
aaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatct
gatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatattt
caatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtaga
ttattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgta
attaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgc
ttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgt
tccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaa
gaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtca
ctttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacagg
agcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaa
taaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtca
cactggctcaccttcgggtgggcctttctgcgtttata