BBa_K199123 1 BBa_K199123 autoinducer synthetase for AHL (no LVA) + Unique Site 2010-03-08T12:00:00Z 2015-05-08T01:11:20Z V. fischeri [Genbank AF170104] Synthesizes 3OC6HSL, which binds to LuxR. Directly downstream of the ATG start codon is an AsiSI restriction site, a 5 bp-spacer, and an AscI restriction site. These two restriction sites allow for unique coding sequences to easily be swapped out of the gene. These unique coding sequences contain frameshift mutations that can be suppressed by 5-bp tRNA suppressors. Whether or not these frameshift mutations are suppressed determines whether or not LuxI will be transcribed properly. false false _295_ 0 5109 9 Not in stock false - false Romina Clemente annotation2067364 1 Unique Site range2067364 1 4 24 BBa_K199123_sequence 1 atggcgatcgcatctgggcgcgccactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaattaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z