LOCUS BBa_K199131 2866 bp DNA linear 18-Nov-2024 DEFINITION Lux positive feedback loop with 2 sites ACCESSION BBa_K199131 VERSION BBa_K199131.1 FEATURES Location/Qualifiers stem_loop 12..55 /label=stem_loop misc_feature 1..80 /label=BBa_B0010 terminator 1..80 /label=BBa_B0010 stem_loop 96..115 /label=T7 TE misc_feature 122..122 /label=stop polyA_site 116..129 /label=polya misc_feature 89..129 /label=BBa_B0012 terminator 89..129 /label=BBa_B0012 misc_feature 138..157 /label=LuxR/HSL promoter 157..162 /label=-35 promoter 179..184 /label=-10 misc_feature 190..190 /label=start misc_feature 138..192 /label=BBa_R0062 promoter 138..192 /label=BBa_R0062 misc_feature 205..208 /label=conserved RBS 201..212 /label=BBa_B0034 misc_feature 222..229 /label=AsiSI Site misc_feature 235..242 /label=AscI Site CDS 219..845 /label=BBa_K199130 misc_feature 1..845 /label=BBa_S04424 misc_feature 858..861 /label=conserved RBS 854..865 /label=BBa_B0034 CDS 872..1546 /label=mrfp1 misc_feature 1553..1577 /label=Help:Barcodes CDS 872..1552 /label=BBa_E1010 stem_loop 1597..1640 /label=stem_loop misc_feature 1586..1665 /label=BBa_B0010 terminator 1586..1665 /label=BBa_B0010 stem_loop 1681..1700 /label=T7 TE misc_feature 1707..1707 /label=stop polyA_site 1701..1714 /label=polya misc_feature 1674..1714 /label=BBa_B0012 terminator 1674..1714 /label=BBa_B0012 misc_feature 872..1714 /label=BBa_I13501 CDS 1723..1810 /label=end of LacI coding region (inactive) misc_feature 1723..1922 /label=BBa_R0010 misc_feature 1811..1848 /label=CAP binding site promoter 1859..1864 /label=-35 promoter 1883..1888 /label=-10 misc_feature 1888..1922 /label=LacI binding site misc_feature 1895..1895 /label=start promoter 1723..1922 /label=BBa_R0010 misc_feature 1935..1938 /label=conserved RBS 1931..1942 /label=BBa_B0034 misc_feature 1949..1950 /label=prefix misc_feature 2122..2122 /label=T misc_feature 2440..2440 /label=A CDS 1949..2698 /label=luxR misc_feature 1949..2704 /label=BBa_C0062 misc_feature 2705..2729 /label=Help:Barcodes CDS 1949..2704 /label=BBa_C0062 stem_loop 2749..2792 /label=stem_loop misc_feature 2738..2817 /label=BBa_B0010 terminator 2738..2817 /label=BBa_B0010 stem_loop 2833..2852 /label=T7 TE misc_feature 2859..2859 /label=stop polyA_site 2853..2866 /label=polya misc_feature 2826..2866 /label=BBa_B0012 terminator 2826..2866 /label=BBa_B0012 misc_feature 1931..2866 /label=BBa_I0462 misc_feature 1723..2866 /label=BBa_K109702 misc_feature 872..2866 /label=BBa_K199113 misc_feature 854..2866 /label=BBa_K199116 ORIGIN 1 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt 61 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct 121 gcgtttatat actagagacc tgtaggatcg tacaggttta cgcaagaaaa tggtttgtta 181 tagtcgaata aatactagag aaagaggaga aatactagat ggcgatcgca tctgggcgcg 241 ccgcaattgc aagcggtcgt gcaaccatta tgattaaaaa aagcgatttt ctggccattc 301 cgagcgaaga atataaaggt attctgagcc tgcgctatca ggtttttaaa cagcgtctgg 361 aatgggatct ggtggttgaa aataatctgg aaagtgatga atatgataat agcaatgccg 421 aatatattta tgcctgtgat gataccgaaa atgttagcgg ttgttggcgt ctgctgccga 481 ccaccggtga ttatatgctg aaaagcgttt ttccggaact gctgggtcag cagagcgcac 541 cgaaagatcc gaatattgtt gaactgagcc gttttgccgt gggtaaaaat agcagcaaaa 601 ttaataatag cgccagcgaa attaccatga aactgtttga agccatttat aaacatgccg 661 ttagccaggg tattaccgaa tatgttaccg ttaccagcac cgcaattgaa cgttttctga 721 aacgtattaa agtgccgtgt catcgtattg gcgataaaga aattcatgtt ctgggcgata 781 ccaaaagcgt tgttctgagc atgccgatta atgaacagtt taaaaaagcc gtgctgaact 841 aataatacta gagaaagagg agaaatacta gatggcttcc tccgaagacg ttatcaaaga 901 gttcatgcgt ttcaaagttc gtatggaagg ttccgttaac ggtcacgagt tcgaaatcga 961 aggtgaaggt gaaggtcgtc cgtacgaagg tacccagacc gctaaactga aagttaccaa 1021 aggtggtccg ctgccgttcg cttgggacat cctgtccccg cagttccagt acggttccaa 1081 agcttacgtt aaacacccgg ctgacatccc ggactacctg aaactgtcct tcccggaagg 1141 tttcaaatgg gaacgtgtta tgaacttcga agacggtggt gttgttaccg ttacccagga 1201 ctcctccctg caagacggtg agttcatcta caaagttaaa ctgcgtggta ccaacttccc 1261 gtccgacggt ccggttatgc agaaaaaaac catgggttgg gaagcttcca ccgaacgtat 1321 gtacccggaa gacggtgctc tgaaaggtga aatcaaaatg cgtctgaaac tgaaagacgg 1381 tggtcactac gacgctgaag ttaaaaccac ctacatggct aaaaaaccgg ttcagctgcc 1441 gggtgcttac aaaaccgaca tcaaactgga catcacctcc cacaacgaag actacaccat 1501 cgttgaacag tacgaacgtg ctgaaggtcg tcactccacc ggtgcttaat aacgctgata 1561 gtgctagtgt agatcgctac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa 1621 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact 1681 ggctcacctt cgggtgggcc tttctgcgtt tatatactag agcaatacgc aaaccgcctc 1741 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag 1801 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt 1861 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca 1921 catactagag aaagaggaga aatactagat gaaaaacata aatgccgacg acacatacag 1981 aataattaat aaaattaaag cttgtagaag caataatgat attaatcaat gcttatctga 2041 tatgactaaa atggtacatt gtgaatatta tttactcgcg atcatttatc ctcattctat 2101 ggttaaatct gatatttcaa tcctagataa ttaccctaaa aaatggaggc aatattatga 2161 tgacgctaat ttaataaaat atgatcctat agtagattat tctaactcca atcattcacc 2221 aattaattgg aatatatttg aaaacaatgc tgtaaataaa aaatctccaa atgtaattaa 2281 agaagcgaaa acatcaggtc ttatcactgg gtttagtttc cctattcata cggctaacaa 2341 tggcttcgga atgcttagtt ttgcacattc agaaaaagac aactatatag atagtttatt 2401 tttacatgcg tgtatgaaca taccattaat tgttccttct ctagttgata attatcgaaa 2461 aataaatata gcaaataata aatcaaacaa cgatttaacc aaaagagaaa aagaatgttt 2521 agcgtgggca tgcgaaggaa aaagctcttg ggatatttca aaaatattag gttgcagtga 2581 gcgtactgtc actttccatt taaccaatgc gcaaatgaaa ctcaatacaa caaaccgctg 2641 ccaaagtatt tctaaagcaa ttttaacagg agcaattgat tgcccatact ttaaaaatta 2701 ataacactga tagtgctagt gtagatcact actagagcca ggcatcaaat aaaacgaaag 2761 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac 2821 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata //