##gff-version 3
##sequence-region BBa_K199131 1 2866
BBa_K199131	.	engineered_region	1	845	.	+	0	ID=BBa_S04424;Name=BBa_S04424
BBa_K199131	.	ribosome_entry_site	201	212	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S04424
BBa_K199131	.	conserved_region	205	208	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K199131	.	CDS	219	845	.	+	0	ID=BBa_K199130;Name=BBa_K199130;Parent=BBa_S04424
BBa_K199131	.	sequence_feature	222	229	.	+	0	ID=annotation2120504;Name=AsiSI Site;Parent=BBa_K199130
BBa_K199131	.	sequence_feature	235	242	.	+	0	ID=annotation2120505;Name=AscI Site;Parent=BBa_K199130
BBa_K199131	.	terminator	1	80	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_S04424
BBa_K199131	.	engineered_region	1	80	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K199131	.	stem_loop	12	55	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K199131	.	terminator	89	129	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_S04424
BBa_K199131	.	engineered_region	89	129	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K199131	.	polyA_site	116	129	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K199131	.	stem_loop	96	115	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K199131	.	stop_codon	122	122	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K199131	.	promoter	138	192	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_S04424
BBa_K199131	.	engineered_region	138	192	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K199131	.	promoter	179	184	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K199131	.	start_codon	190	190	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K199131	.	non_covalent_binding_site	138	157	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K199131	.	promoter	157	162	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K199131	.	engineered_region	854	2866	.	+	0	ID=BBa_K199116;Name=BBa_K199116
BBa_K199131	.	ribosome_entry_site	854	865	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K199116
BBa_K199131	.	conserved_region	858	861	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K199131	.	engineered_region	872	2866	.	+	0	ID=BBa_K199113;Name=BBa_K199113;Parent=BBa_K199116
BBa_K199131	.	engineered_region	1723	2866	.	+	0	ID=BBa_K109702;Name=BBa_K109702;Parent=BBa_K199113
BBa_K199131	.	promoter	1723	1922	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_K109702
BBa_K199131	.	CDS	1723	1810	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K199131	.	promoter	1883	1888	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K199131	.	non_covalent_binding_site	1888	1922	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K199131	.	engineered_region	1723	1922	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K199131	.	promoter	1859	1864	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K199131	.	start_codon	1895	1895	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K199131	.	non_covalent_binding_site	1811	1848	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K199131	.	engineered_region	1931	2866	.	+	0	ID=BBa_I0462;Name=BBa_I0462;Parent=BBa_K109702
BBa_K199131	.	terminator	2738	2817	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0462
BBa_K199131	.	engineered_region	2738	2817	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K199131	.	stem_loop	2749	2792	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K199131	.	terminator	2826	2866	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0462
BBa_K199131	.	engineered_region	2826	2866	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K199131	.	polyA_site	2853	2866	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K199131	.	stem_loop	2833	2852	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K199131	.	stop_codon	2859	2859	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K199131	.	CDS	1949	2704	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_I0462
BBa_K199131	.	point_mutation	2440	2440	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K199131	.	engineered_tag	2705	2729	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K199131	.	engineered_region	1949	2704	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K199131	.	point_mutation	2122	2122	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K199131	.	engineered_region	1949	1950	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K199131	.	CDS	1949	2698	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K199131	.	ribosome_entry_site	1931	1942	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0462
BBa_K199131	.	conserved_region	1935	1938	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K199131	.	engineered_region	872	1714	.	+	0	ID=BBa_I13501;Name=BBa_I13501;Parent=BBa_K199113
BBa_K199131	.	terminator	1674	1714	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13501
BBa_K199131	.	engineered_region	1674	1714	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K199131	.	polyA_site	1701	1714	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K199131	.	stem_loop	1681	1700	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K199131	.	stop_codon	1707	1707	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K199131	.	terminator	1586	1665	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13501
BBa_K199131	.	engineered_region	1586	1665	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K199131	.	stem_loop	1597	1640	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K199131	.	CDS	872	1552	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_I13501
BBa_K199131	.	CDS	872	1546	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K199131	.	engineered_tag	1553	1577	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
>BBa_K199131
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtt
tacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatactagatggcgatcgcatctgggc
gcgccgcaattgcaagcggtcgtgcaaccattatgattaaaaaaagcgattttctggccattccgagcgaagaatataa
aggtattctgagcctgcgctatcaggtttttaaacagcgtctggaatgggatctggtggttgaaaataatctggaaagt
gatgaatatgataatagcaatgccgaatatatttatgcctgtgatgataccgaaaatgttagcggttgttggcgtctgc
tgccgaccaccggtgattatatgctgaaaagcgtttttccggaactgctgggtcagcagagcgcaccgaaagatccgaa
tattgttgaactgagccgttttgccgtgggtaaaaatagcagcaaaattaataatagcgccagcgaaattaccatgaaa
ctgtttgaagccatttataaacatgccgttagccagggtattaccgaatatgttaccgttaccagcaccgcaattgaac
gttttctgaaacgtattaaagtgccgtgtcatcgtattggcgataaagaaattcatgttctgggcgataccaaaagcgt
tgttctgagcatgccgattaatgaacagtttaaaaaagccgtgctgaactaataatactagagaaagaggagaaatact
agatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacga
gttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggt
ccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgaca
tcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgt
taccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtcc
gacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctga
aaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaa
aaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatc
gttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctac
tagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaac
gctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcaatacgcaaaccgcc
tctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaac
gcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaa
ttgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacaca
tacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatgg
tacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataatta
ccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaat
cattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaa
catcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattc
agaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgat
aattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtggg
catgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaa
tgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgccca
tactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctca
gtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcg
ggtgggcctttctgcgtttata