LOCUS BBa_K199135 2845 bp DNA linear 20-Dec-2024 DEFINITION Optimized positive feedback RFP generator ACCESSION BBa_K199135 VERSION BBa_K199135.1 FEATURES Location/Qualifiers stem_loop 12..55 /label=stem_loop misc_feature 1..80 /label=BBa_B0010 terminator 1..80 /label=BBa_B0010 stem_loop 96..115 /label=T7 TE misc_feature 122..122 /label=stop polyA_site 116..129 /label=polya misc_feature 89..129 /label=BBa_B0012 terminator 89..129 /label=BBa_B0012 misc_feature 138..157 /label=LuxR/HSL promoter 157..162 /label=-35 promoter 179..184 /label=-10 misc_feature 190..190 /label=start misc_feature 138..192 /label=BBa_R0062 promoter 138..192 /label=BBa_R0062 misc_feature 205..208 /label=conserved RBS 201..212 /label=BBa_B0034 CDS 219..824 /label=BBa_K199125 CDS 1..824 /label=BBa_K199127 misc_feature 837..840 /label=conserved RBS 833..844 /label=BBa_B0034 CDS 851..1525 /label=mrfp1 misc_feature 1532..1556 /label=Help:Barcodes CDS 851..1531 /label=BBa_E1010 stem_loop 1576..1619 /label=stem_loop misc_feature 1565..1644 /label=BBa_B0010 terminator 1565..1644 /label=BBa_B0010 stem_loop 1660..1679 /label=T7 TE misc_feature 1686..1686 /label=stop polyA_site 1680..1693 /label=polya misc_feature 1653..1693 /label=BBa_B0012 terminator 1653..1693 /label=BBa_B0012 misc_feature 851..1693 /label=BBa_I13501 CDS 1702..1789 /label=end of LacI coding region (inactive) misc_feature 1702..1901 /label=BBa_R0010 misc_feature 1790..1827 /label=CAP binding site promoter 1838..1843 /label=-35 promoter 1862..1867 /label=-10 misc_feature 1867..1901 /label=LacI binding site misc_feature 1874..1874 /label=start promoter 1702..1901 /label=BBa_R0010 misc_feature 1914..1917 /label=conserved RBS 1910..1921 /label=BBa_B0034 misc_feature 1928..1929 /label=prefix misc_feature 2101..2101 /label=T misc_feature 2419..2419 /label=A CDS 1928..2677 /label=luxR misc_feature 1928..2683 /label=BBa_C0062 misc_feature 2684..2708 /label=Help:Barcodes CDS 1928..2683 /label=BBa_C0062 stem_loop 2728..2771 /label=stem_loop misc_feature 2717..2796 /label=BBa_B0010 terminator 2717..2796 /label=BBa_B0010 stem_loop 2812..2831 /label=T7 TE misc_feature 2838..2838 /label=stop polyA_site 2832..2845 /label=polya misc_feature 2805..2845 /label=BBa_B0012 terminator 2805..2845 /label=BBa_B0012 misc_feature 1910..2845 /label=BBa_I0462 misc_feature 1702..2845 /label=BBa_K109702 misc_feature 851..2845 /label=BBa_K199113 misc_feature 833..2845 /label=BBa_K199116 ORIGIN 1 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt 61 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct 121 gcgtttatat actagagacc tgtaggatcg tacaggttta cgcaagaaaa tggtttgtta 181 tagtcgaata aatactagag aaagaggaga aatactagat ggcaattgca agcggtcgtg 241 caaccattat gattaaaaaa agcgattttc tggccattcc gagcgaagaa tataaaggta 301 ttctgagcct gcgctatcag gtttttaaac agcgtctgga atgggatctg gtggttgaaa 361 ataatctgga aagtgatgaa tatgataata gcaatgccga atatatttat gcctgtgatg 421 ataccgaaaa tgttagcggt tgttggcgtc tgctgccgac caccggtgat tatatgctga 481 aaagcgtttt tccggaactg ctgggtcagc agagcgcacc gaaagatccg aatattgttg 541 aactgagccg ttttgccgtg ggtaaaaata gcagcaaaat taataatagc gccagcgaaa 601 ttaccatgaa actgtttgaa gccatttata aacatgccgt tagccagggt attaccgaat 661 atgttaccgt taccagcacc gcaattgaac gttttctgaa acgtattaaa gtgccgtgtc 721 atcgtattgg cgataaagaa attcatgttc tgggcgatac caaaagcgtt gttctgagca 781 tgccgattaa tgaacagttt aaaaaagccg tgctgaacta ataatactag agaaagagga 841 gaaatactag atggcttcct ccgaagacgt tatcaaagag ttcatgcgtt tcaaagttcg 901 tatggaaggt tccgttaacg gtcacgagtt cgaaatcgaa ggtgaaggtg aaggtcgtcc 961 gtacgaaggt acccagaccg ctaaactgaa agttaccaaa ggtggtccgc tgccgttcgc 1021 ttgggacatc ctgtccccgc agttccagta cggttccaaa gcttacgtta aacacccggc 1081 tgacatcccg gactacctga aactgtcctt cccggaaggt ttcaaatggg aacgtgttat 1141 gaacttcgaa gacggtggtg ttgttaccgt tacccaggac tcctccctgc aagacggtga 1201 gttcatctac aaagttaaac tgcgtggtac caacttcccg tccgacggtc cggttatgca 1261 gaaaaaaacc atgggttggg aagcttccac cgaacgtatg tacccggaag acggtgctct 1321 gaaaggtgaa atcaaaatgc gtctgaaact gaaagacggt ggtcactacg acgctgaagt 1381 taaaaccacc tacatggcta aaaaaccggt tcagctgccg ggtgcttaca aaaccgacat 1441 caaactggac atcacctccc acaacgaaga ctacaccatc gttgaacagt acgaacgtgc 1501 tgaaggtcgt cactccaccg gtgcttaata acgctgatag tgctagtgta gatcgctact 1561 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 1621 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct 1681 ttctgcgttt atatactaga gcaatacgca aaccgcctct ccccgcgcgt tggccgattc 1741 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa 1801 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc 1861 gtatgttgtg tggaattgtg agcggataac aatttcacac atactagaga aagaggagaa 1921 atactagatg aaaaacataa atgccgacga cacatacaga ataattaata aaattaaagc 1981 ttgtagaagc aataatgata ttaatcaatg cttatctgat atgactaaaa tggtacattg 2041 tgaatattat ttactcgcga tcatttatcc tcattctatg gttaaatctg atatttcaat 2101 cctagataat taccctaaaa aatggaggca atattatgat gacgctaatt taataaaata 2161 tgatcctata gtagattatt ctaactccaa tcattcacca attaattgga atatatttga 2221 aaacaatgct gtaaataaaa aatctccaaa tgtaattaaa gaagcgaaaa catcaggtct 2281 tatcactggg tttagtttcc ctattcatac ggctaacaat ggcttcggaa tgcttagttt 2341 tgcacattca gaaaaagaca actatataga tagtttattt ttacatgcgt gtatgaacat 2401 accattaatt gttccttctc tagttgataa ttatcgaaaa ataaatatag caaataataa 2461 atcaaacaac gatttaacca aaagagaaaa agaatgttta gcgtgggcat gcgaaggaaa 2521 aagctcttgg gatatttcaa aaatattagg ttgcagtgag cgtactgtca ctttccattt 2581 aaccaatgcg caaatgaaac tcaatacaac aaaccgctgc caaagtattt ctaaagcaat 2641 tttaacagga gcaattgatt gcccatactt taaaaattaa taacactgat agtgctagtg 2701 tagatcacta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc 2761 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct 2821 tcgggtgggc ctttctgcgt ttata //