##gff-version 3
##sequence-region BBa_K199135 1 2845
BBa_K199135	.	CDS	1	824	.	+	0	ID=BBa_K199127;Name=BBa_K199127
BBa_K199135	.	ribosome_entry_site	201	212	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K199127
BBa_K199135	.	conserved_region	205	208	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K199135	.	terminator	89	129	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K199127
BBa_K199135	.	stop_codon	122	122	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K199135	.	engineered_region	89	129	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K199135	.	stem_loop	96	115	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K199135	.	polyA_site	116	129	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K199135	.	terminator	1	80	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K199127
BBa_K199135	.	stem_loop	12	55	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K199135	.	engineered_region	1	80	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K199135	.	promoter	138	192	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_K199127
BBa_K199135	.	non_covalent_binding_site	138	157	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K199135	.	promoter	179	184	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K199135	.	promoter	157	162	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K199135	.	start_codon	190	190	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K199135	.	engineered_region	138	192	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K199135	.	CDS	219	824	.	+	0	ID=BBa_K199125;Name=BBa_K199125;Parent=BBa_K199127
BBa_K199135	.	engineered_region	833	2845	.	+	0	ID=BBa_K199116;Name=BBa_K199116
BBa_K199135	.	ribosome_entry_site	833	844	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K199116
BBa_K199135	.	conserved_region	837	840	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K199135	.	engineered_region	851	2845	.	+	0	ID=BBa_K199113;Name=BBa_K199113;Parent=BBa_K199116
BBa_K199135	.	engineered_region	851	1693	.	+	0	ID=BBa_I13501;Name=BBa_I13501;Parent=BBa_K199113
BBa_K199135	.	terminator	1653	1693	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13501
BBa_K199135	.	stop_codon	1686	1686	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K199135	.	engineered_region	1653	1693	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K199135	.	stem_loop	1660	1679	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K199135	.	polyA_site	1680	1693	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K199135	.	CDS	851	1531	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_I13501
BBa_K199135	.	engineered_tag	1532	1556	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K199135	.	CDS	851	1525	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K199135	.	terminator	1565	1644	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13501
BBa_K199135	.	stem_loop	1576	1619	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K199135	.	engineered_region	1565	1644	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K199135	.	engineered_region	1702	2845	.	+	0	ID=BBa_K109702;Name=BBa_K109702;Parent=BBa_K199113
BBa_K199135	.	promoter	1702	1901	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_K109702
BBa_K199135	.	non_covalent_binding_site	1790	1827	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K199135	.	promoter	1838	1843	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K199135	.	engineered_region	1702	1901	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K199135	.	CDS	1702	1789	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K199135	.	promoter	1862	1867	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K199135	.	start_codon	1874	1874	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K199135	.	non_covalent_binding_site	1867	1901	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K199135	.	engineered_region	1910	2845	.	+	0	ID=BBa_I0462;Name=BBa_I0462;Parent=BBa_K109702
BBa_K199135	.	terminator	2717	2796	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0462
BBa_K199135	.	stem_loop	2728	2771	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K199135	.	engineered_region	2717	2796	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K199135	.	ribosome_entry_site	1910	1921	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0462
BBa_K199135	.	conserved_region	1914	1917	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K199135	.	CDS	1928	2683	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_I0462
BBa_K199135	.	CDS	1928	2677	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K199135	.	engineered_region	1928	2683	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K199135	.	engineered_region	1928	1929	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K199135	.	point_mutation	2101	2101	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K199135	.	engineered_tag	2684	2708	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K199135	.	point_mutation	2419	2419	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K199135	.	terminator	2805	2845	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0462
BBa_K199135	.	stop_codon	2838	2838	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K199135	.	engineered_region	2805	2845	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K199135	.	stem_loop	2812	2831	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K199135	.	polyA_site	2832	2845	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
>BBa_K199135
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtt
tacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatactagatggcaattgcaagcggtc
gtgcaaccattatgattaaaaaaagcgattttctggccattccgagcgaagaatataaaggtattctgagcctgcgcta
tcaggtttttaaacagcgtctggaatgggatctggtggttgaaaataatctggaaagtgatgaatatgataatagcaat
gccgaatatatttatgcctgtgatgataccgaaaatgttagcggttgttggcgtctgctgccgaccaccggtgattata
tgctgaaaagcgtttttccggaactgctgggtcagcagagcgcaccgaaagatccgaatattgttgaactgagccgttt
tgccgtgggtaaaaatagcagcaaaattaataatagcgccagcgaaattaccatgaaactgtttgaagccatttataaa
catgccgttagccagggtattaccgaatatgttaccgttaccagcaccgcaattgaacgttttctgaaacgtattaaag
tgccgtgtcatcgtattggcgataaagaaattcatgttctgggcgataccaaaagcgttgttctgagcatgccgattaa
tgaacagtttaaaaaagccgtgctgaactaataatactagagaaagaggagaaatactagatggcttcctccgaagacg
ttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaagg
tgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggac
atcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgt
ccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctc
cctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaa
aaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtc
tgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccggg
tgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgct
gaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaa
acgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacact
ggctcaccttcgggtgggcctttctgcgtttatatactagagcaatacgcaaaccgcctctccccgcgcgttggccgat
tcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctc
actcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttc
acacatactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaatt
aaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttac
tcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaata
ttatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaat
atatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggt
ttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatataga
tagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatata
gcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctctt
gggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatac
aacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacac
tgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcg
ttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
a