##gff-version 3
##sequence-region BBa_K199144 1 2871
BBa_K199144	.	engineered_region	1	850	.	+	0	ID=BBa_S04425;Name=BBa_S04425
BBa_K199144	.	terminator	1	80	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_S04425
BBa_K199144	.	stem_loop	12	55	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K199144	.	engineered_region	1	80	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K199144	.	ribosome_entry_site	201	212	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S04425
BBa_K199144	.	conserved_region	205	208	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K199144	.	CDS	219	850	.	+	0	ID=BBa_K199142;Name=BBa_K199142;Parent=BBa_S04425
BBa_K199144	.	terminator	89	129	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_S04425
BBa_K199144	.	stem_loop	96	115	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K199144	.	polyA_site	116	129	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K199144	.	stop_codon	122	122	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K199144	.	engineered_region	89	129	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K199144	.	promoter	138	192	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_S04425
BBa_K199144	.	promoter	157	162	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K199144	.	engineered_region	138	192	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K199144	.	start_codon	190	190	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K199144	.	promoter	179	184	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K199144	.	non_covalent_binding_site	138	157	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K199144	.	engineered_region	859	2871	.	+	0	ID=BBa_K199116;Name=BBa_K199116
BBa_K199144	.	ribosome_entry_site	859	870	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K199116
BBa_K199144	.	conserved_region	863	866	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K199144	.	engineered_region	877	2871	.	+	0	ID=BBa_K199113;Name=BBa_K199113;Parent=BBa_K199116
BBa_K199144	.	engineered_region	877	1719	.	+	0	ID=BBa_I13501;Name=BBa_I13501;Parent=BBa_K199113
BBa_K199144	.	terminator	1679	1719	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13501
BBa_K199144	.	stem_loop	1686	1705	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K199144	.	polyA_site	1706	1719	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K199144	.	stop_codon	1712	1712	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K199144	.	engineered_region	1679	1719	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K199144	.	terminator	1591	1670	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13501
BBa_K199144	.	stem_loop	1602	1645	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K199144	.	engineered_region	1591	1670	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K199144	.	CDS	877	1557	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_I13501
BBa_K199144	.	CDS	877	1551	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K199144	.	engineered_tag	1558	1582	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K199144	.	engineered_region	1728	2871	.	+	0	ID=BBa_K109702;Name=BBa_K109702;Parent=BBa_K199113
BBa_K199144	.	promoter	1728	1927	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_K109702
BBa_K199144	.	CDS	1728	1815	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K199144	.	promoter	1888	1893	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K199144	.	non_covalent_binding_site	1816	1853	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K199144	.	non_covalent_binding_site	1893	1927	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K199144	.	promoter	1864	1869	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K199144	.	engineered_region	1728	1927	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K199144	.	start_codon	1900	1900	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K199144	.	engineered_region	1936	2871	.	+	0	ID=BBa_I0462;Name=BBa_I0462;Parent=BBa_K109702
BBa_K199144	.	CDS	1954	2709	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_I0462
BBa_K199144	.	engineered_region	1954	1955	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K199144	.	engineered_tag	2710	2734	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K199144	.	CDS	1954	2703	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K199144	.	point_mutation	2127	2127	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K199144	.	point_mutation	2445	2445	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K199144	.	engineered_region	1954	2709	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K199144	.	terminator	2831	2871	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0462
BBa_K199144	.	stem_loop	2838	2857	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K199144	.	polyA_site	2858	2871	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K199144	.	stop_codon	2864	2864	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K199144	.	engineered_region	2831	2871	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K199144	.	ribosome_entry_site	1936	1947	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0462
BBa_K199144	.	conserved_region	1940	1943	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K199144	.	terminator	2743	2822	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0462
BBa_K199144	.	stem_loop	2754	2797	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K199144	.	engineered_region	2743	2822	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_K199144
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtt
tacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatactagatggcgatcgcaccacttc
tgggcgcgccgcaattgcaagcggtcgtgcaaccattatgattaaaaaaagcgattttctggccattccgagcgaagaa
tataaaggtattctgagcctgcgctatcaggtttttaaacagcgtctggaatgggatctggtggttgaaaataatctgg
aaagtgatgaatatgataatagcaatgccgaatatatttatgcctgtgatgataccgaaaatgttagcggttgttggcg
tctgctgccgaccaccggtgattatatgctgaaaagcgtttttccggaactgctgggtcagcagagcgcaccgaaagat
ccgaatattgttgaactgagccgttttgccgtgggtaaaaatagcagcaaaattaataatagcgccagcgaaattacca
tgaaactgtttgaagccatttataaacatgccgttagccagggtattaccgaatatgttaccgttaccagcaccgcaat
tgaacgttttctgaaacgtattaaagtgccgtgtcatcgtattggcgataaagaaattcatgttctgggcgataccaaa
agcgttgttctgagcatgccgattaatgaacagtttaaaaaagccgtgctgaactaataatactagagaaagaggagaa
atactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggt
cacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaag
gtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggc
tgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggt
gttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcc
cgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgc
tctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatg
gctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactaca
ccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatc
gctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcgg
tgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcaatacgcaaa
ccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagc
gcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtg
tggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgaaaaacataaatgccgacg
acacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaa
aatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagat
aattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaact
ccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagc
gaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgca
cattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctag
ttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagc
gtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccattta
accaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgatt
gcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaag
gctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcac
cttcgggtgggcctttctgcgtttata