LOCUS BBa_K199147 2871 bp DNA linear 20-Dec-2024 DEFINITION Lux positive feedback loop (CCAUC 1-SAT) ACCESSION BBa_K199147 VERSION BBa_K199147.1 FEATURES Location/Qualifiers stem_loop 12..55 /label=stem_loop misc_feature 1..80 /label=BBa_B0010 terminator 1..80 /label=BBa_B0010 stem_loop 96..115 /label=T7 TE misc_feature 122..122 /label=stop polyA_site 116..129 /label=polya misc_feature 89..129 /label=BBa_B0012 terminator 89..129 /label=BBa_B0012 misc_feature 138..157 /label=LuxR/HSL promoter 157..162 /label=-35 promoter 179..184 /label=-10 misc_feature 190..190 /label=start misc_feature 138..192 /label=BBa_R0062 promoter 138..192 /label=BBa_R0062 misc_feature 205..208 /label=conserved RBS 201..212 /label=BBa_B0034 promoter 1..212 /label=BBa_K199114 CDS 219..850 /label=BBa_K199146 misc_feature 863..866 /label=conserved RBS 859..870 /label=BBa_B0034 CDS 877..1551 /label=mrfp1 misc_feature 1558..1582 /label=Help:Barcodes CDS 877..1557 /label=BBa_E1010 stem_loop 1602..1645 /label=stem_loop misc_feature 1591..1670 /label=BBa_B0010 terminator 1591..1670 /label=BBa_B0010 stem_loop 1686..1705 /label=T7 TE misc_feature 1712..1712 /label=stop polyA_site 1706..1719 /label=polya misc_feature 1679..1719 /label=BBa_B0012 terminator 1679..1719 /label=BBa_B0012 misc_feature 877..1719 /label=BBa_I13501 CDS 1728..1815 /label=end of LacI coding region (inactive) misc_feature 1728..1927 /label=BBa_R0010 misc_feature 1816..1853 /label=CAP binding site promoter 1864..1869 /label=-35 promoter 1888..1893 /label=-10 misc_feature 1893..1927 /label=LacI binding site misc_feature 1900..1900 /label=start promoter 1728..1927 /label=BBa_R0010 misc_feature 1940..1943 /label=conserved RBS 1936..1947 /label=BBa_B0034 misc_feature 1954..1955 /label=prefix misc_feature 2127..2127 /label=T misc_feature 2445..2445 /label=A CDS 1954..2703 /label=luxR misc_feature 1954..2709 /label=BBa_C0062 misc_feature 2710..2734 /label=Help:Barcodes CDS 1954..2709 /label=BBa_C0062 stem_loop 2754..2797 /label=stem_loop misc_feature 2743..2822 /label=BBa_B0010 terminator 2743..2822 /label=BBa_B0010 stem_loop 2838..2857 /label=T7 TE misc_feature 2864..2864 /label=stop polyA_site 2858..2871 /label=polya misc_feature 2831..2871 /label=BBa_B0012 terminator 2831..2871 /label=BBa_B0012 misc_feature 1936..2871 /label=BBa_I0462 misc_feature 1728..2871 /label=BBa_K109702 misc_feature 877..2871 /label=BBa_K199113 misc_feature 859..2871 /label=BBa_K199116 ORIGIN 1 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt 61 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct 121 gcgtttatat actagagacc tgtaggatcg tacaggttta cgcaagaaaa tggtttgtta 181 tagtcgaata aatactagag aaagaggaga aatactagat ggcgatcgca ccatctctgg 241 gcgcgccgca attgcaagcg gtcgtgcaac cattatgatt aaaaaaagcg attttctggc 301 cattccgagc gaagaatata aaggtattct gagcctgcgc tatcaggttt ttaaacagcg 361 tctggaatgg gatctggtgg ttgaaaataa tctggaaagt gatgaatatg ataatagcaa 421 tgccgaatat atttatgcct gtgatgatac cgaaaatgtt agcggttgtt ggcgtctgct 481 gccgaccacc ggtgattata tgctgaaaag cgtttttccg gaactgctgg gtcagcagag 541 cgcaccgaaa gatccgaata ttgttgaact gagccgtttt gccgtgggta aaaatagcag 601 caaaattaat aatagcgcca gcgaaattac catgaaactg tttgaagcca tttataaaca 661 tgccgttagc cagggtatta ccgaatatgt taccgttacc agcaccgcaa ttgaacgttt 721 tctgaaacgt attaaagtgc cgtgtcatcg tattggcgat aaagaaattc atgttctggg 781 cgataccaaa agcgttgttc tgagcatgcc gattaatgaa cagtttaaaa aagccgtgct 841 gaactaataa tactagagaa agaggagaaa tactagatgg cttcctccga agacgttatc 901 aaagagttca tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca cgagttcgaa 961 atcgaaggtg aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa actgaaagtt 1021 accaaaggtg gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt ccagtacggt 1081 tccaaagctt acgttaaaca cccggctgac atcccggact acctgaaact gtccttcccg 1141 gaaggtttca aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt taccgttacc 1201 caggactcct ccctgcaaga cggtgagttc atctacaaag ttaaactgcg tggtaccaac 1261 ttcccgtccg acggtccggt tatgcagaaa aaaaccatgg gttgggaagc ttccaccgaa 1321 cgtatgtacc cggaagacgg tgctctgaaa ggtgaaatca aaatgcgtct gaaactgaaa 1381 gacggtggtc actacgacgc tgaagttaaa accacctaca tggctaaaaa accggttcag 1441 ctgccgggtg cttacaaaac cgacatcaaa ctggacatca cctcccacaa cgaagactac 1501 accatcgttg aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc ttaataacgc 1561 tgatagtgct agtgtagatc gctactagag ccaggcatca aataaaacga aaggctcagt 1621 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc 1681 acactggctc accttcgggt gggcctttct gcgtttatat actagagcaa tacgcaaacc 1741 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg 1801 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca 1861 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt 1921 tcacacatac tagagaaaga ggagaaatac tagatgaaaa acataaatgc cgacgacaca 1981 tacagaataa ttaataaaat taaagcttgt agaagcaata atgatattaa tcaatgctta 2041 tctgatatga ctaaaatggt acattgtgaa tattatttac tcgcgatcat ttatcctcat 2101 tctatggtta aatctgatat ttcaatccta gataattacc ctaaaaaatg gaggcaatat 2161 tatgatgacg ctaatttaat aaaatatgat cctatagtag attattctaa ctccaatcat 2221 tcaccaatta attggaatat atttgaaaac aatgctgtaa ataaaaaatc tccaaatgta 2281 attaaagaag cgaaaacatc aggtcttatc actgggttta gtttccctat tcatacggct 2341 aacaatggct tcggaatgct tagttttgca cattcagaaa aagacaacta tatagatagt 2401 ttatttttac atgcgtgtat gaacatacca ttaattgttc cttctctagt tgataattat 2461 cgaaaaataa atatagcaaa taataaatca aacaacgatt taaccaaaag agaaaaagaa 2521 tgtttagcgt gggcatgcga aggaaaaagc tcttgggata tttcaaaaat attaggttgc 2581 agtgagcgta ctgtcacttt ccatttaacc aatgcgcaaa tgaaactcaa tacaacaaac 2641 cgctgccaaa gtatttctaa agcaatttta acaggagcaa ttgattgccc atactttaaa 2701 aattaataac actgatagtg ctagtgtaga tcactactag agccaggcat caaataaaac 2761 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc 2821 tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a //