LOCUS       BBa_K199152             1874 bp    DNA     linear       28-Jun-2024
DEFINITION  Measures the reverse activity of pLux in the presence of LuxR
ACCESSION   BBa_K199152
VERSION     BBa_K199152.1  
FEATURES             Location/Qualifiers
     CDS             1..678
                     /label=mRFP reverse
     primer_bind     659..678
                     /label=Spe mRFP F
     primer_bind     1..20
                     /label=Xba mRFP R
     misc_feature    1..678
                     /label=BBa_J31008
     RBS             687..701
                     /label=Reverse RBS
     RBS             687..701
                     /label=BBa_J44001
     misc_feature    710..729
                     /label=LuxR/HSL
     promoter        729..734
                     /label=-35
     promoter        751..756
                     /label=-10
     misc_feature    762..762
                     /label=start
     misc_feature    710..764
                     /label=BBa_R0062
     promoter        710..764
                     /label=BBa_R0062
     stem_loop       780..799
                     /label=T7 TE
     misc_feature    806..806
                     /label=stop
     polyA_site      800..813
                     /label=polya
     misc_feature    773..813
                     /label=BBa_B0012
     terminator      773..813
                     /label=BBa_B0012
     stem_loop       834..856
                     /label=stem_loop
     misc_feature    822..867
                     /label=BBa_B0011
     terminator      822..867
                     /label=BBa_B0011
     misc_feature    1..867
                     /label=BBa_S04429
     misc_feature    878..894
                     /label=lac O1
     promoter        895..900
                     /label=-35
     misc_feature    901..917
                     /label=lac O1
     promoter        918..923
                     /label=-10
     misc_feature    876..929
                     /label=BBa_R0011
     promoter        876..929
                     /label=BBa_R0011
     misc_feature    943..946
                     /label=conserved
     RBS             939..950
                     /label=BBa_B0034
     misc_feature    957..958
                     /label=prefix
     misc_feature    1130..1130
                     /label=T
     misc_feature    1448..1448
                     /label=A
     CDS             957..1706
                     /label=luxR
     misc_feature    957..1712
                     /label=BBa_C0062
     misc_feature    1713..1737
                     /label=Help:Barcodes
     CDS             957..1712
                     /label=BBa_C0062
     stem_loop       1757..1800
                     /label=stem_loop
     misc_feature    1746..1825
                     /label=BBa_B0010
     terminator      1746..1825
                     /label=BBa_B0010
     stem_loop       1841..1860
                     /label=T7 TE
     misc_feature    1867..1867
                     /label=stop
     polyA_site      1861..1874
                     /label=polya
     misc_feature    1834..1874
                     /label=BBa_B0012
     terminator      1834..1874
                     /label=BBa_B0012
     misc_feature    939..1874
                     /label=BBa_I0462
     misc_feature    876..1874
                     /label=BBa_K199103
ORIGIN
        1 ttaagcaccg gtggagtgac gaccttcagc acgttcgtac tgttcaacga tggtgtagtc
       61 ttcgttgtgg gaggtgatgt ccagtttgat gtcggttttg taagcacccg gcagctgaac
      121 cggtttttta gccatgtagg tggttttaac ttcagcgtcg tagtgaccac cgtctttcag
      181 tttcagacgc attttgattt cacctttcag agcaccgtct tccgggtaca tacgttcggt
      241 ggaagcttcc caacccatgg tttttttctg cataaccgga ccgtcggacg ggaagttggt
      301 accacgcagt ttaactttgt agatgaactc accgtcttgc agggaggagt cctgggtaac
      361 ggtaacaaca ccaccgtctt cgaagttcat aacacgttcc catttgaaac cttccgggaa
      421 ggacagtttc aggtagtccg ggatgtcagc cgggtgttta acgtaagctt tggaaccgta
      481 ctggaactgc ggggacagga tgtcccaagc gaacggcagc ggaccacctt tggtaacttt
      541 cagtttagcg gtctgggtac cttcgtacgg acgaccttca ccttcacctt cgatttcgaa
      601 ctcgtgaccg ttaacggaac cttccatacg aactttgaaa cgcatgaact ctttgataac
      661 gtcttcggag gaagccatta ctagagtttc tcctctttaa ttactagaga cctgtaggat
      721 cgtacaggtt tacgcaagaa aatggtttgt tatagtcgaa taaatactag agtcacactg
      781 gctcaccttc gggtgggcct ttctgcgttt atatactaga gagagaatat aaaaagccag
      841 attattaatc cggctttttt attattttac tagagaattg tgagcggata acaattgaca
      901 ttgtgagcgg ataacaagat actgagcaca tactagagaa agaggagaaa tactagatga
      961 aaaacataaa tgccgacgac acatacagaa taattaataa aattaaagct tgtagaagca
     1021 ataatgatat taatcaatgc ttatctgata tgactaaaat ggtacattgt gaatattatt
     1081 tactcgcgat catttatcct cattctatgg ttaaatctga tatttcaatc ctagataatt
     1141 accctaaaaa atggaggcaa tattatgatg acgctaattt aataaaatat gatcctatag
     1201 tagattattc taactccaat cattcaccaa ttaattggaa tatatttgaa aacaatgctg
     1261 taaataaaaa atctccaaat gtaattaaag aagcgaaaac atcaggtctt atcactgggt
     1321 ttagtttccc tattcatacg gctaacaatg gcttcggaat gcttagtttt gcacattcag
     1381 aaaaagacaa ctatatagat agtttatttt tacatgcgtg tatgaacata ccattaattg
     1441 ttccttctct agttgataat tatcgaaaaa taaatatagc aaataataaa tcaaacaacg
     1501 atttaaccaa aagagaaaaa gaatgtttag cgtgggcatg cgaaggaaaa agctcttggg
     1561 atatttcaaa aatattaggt tgcagtgagc gtactgtcac tttccattta accaatgcgc
     1621 aaatgaaact caatacaaca aaccgctgcc aaagtatttc taaagcaatt ttaacaggag
     1681 caattgattg cccatacttt aaaaattaat aacactgata gtgctagtgt agatcactac
     1741 tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat
     1801 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc
     1861 tttctgcgtt tata
//