##gff-version 3
##sequence-region BBa_K199152 1 1874
BBa_K199152	.	engineered_region	1	867	.	+	0	ID=BBa_S04429;Name=BBa_S04429
BBa_K199152	.	terminator	773	813	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_S04429
BBa_K199152	.	polyA_site	800	813	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K199152	.	engineered_region	773	813	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K199152	.	stem_loop	780	799	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K199152	.	stop_codon	806	806	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K199152	.	engineered_region	1	678	.	+	0	ID=BBa_J31008;Name=BBa_J31008;Parent=BBa_S04429
BBa_K199152	.	CDS	1	678	.	+	0	ID=annotation1911761;Name=mRFP reverse;Parent=BBa_J31008
BBa_K199152	.	primer_binding_site	659	678	.	+	0	ID=annotation1911764;Name=Spe mRFP F;Parent=BBa_J31008
BBa_K199152	.	primer_binding_site	1	20	.	+	0	ID=annotation1911765;Name=Xba mRFP R;Parent=BBa_J31008
BBa_K199152	.	ribosome_entry_site	687	701	.	+	0	ID=BBa_J44001;Name=BBa_J44001;Parent=BBa_S04429
BBa_K199152	.	ribosome_entry_site	687	701	.	+	0	ID=annotation1893199;Name=Reverse RBS;Parent=BBa_J44001
BBa_K199152	.	terminator	822	867	.	+	0	ID=BBa_B0011;Name=BBa_B0011;Parent=BBa_S04429
BBa_K199152	.	stem_loop	834	856	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_K199152	.	engineered_region	822	867	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_K199152	.	promoter	710	764	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_S04429
BBa_K199152	.	non_covalent_binding_site	710	729	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K199152	.	promoter	751	756	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K199152	.	promoter	729	734	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K199152	.	start_codon	762	762	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K199152	.	engineered_region	710	764	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K199152	.	engineered_region	876	1874	.	+	0	ID=BBa_K199103;Name=BBa_K199103
BBa_K199152	.	engineered_region	939	1874	.	+	0	ID=BBa_I0462;Name=BBa_I0462;Parent=BBa_K199103
BBa_K199152	.	terminator	1746	1825	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0462
BBa_K199152	.	engineered_region	1746	1825	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K199152	.	stem_loop	1757	1800	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K199152	.	CDS	957	1712	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_I0462
BBa_K199152	.	engineered_region	957	1712	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K199152	.	engineered_region	957	958	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K199152	.	CDS	957	1706	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K199152	.	point_mutation	1130	1130	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K199152	.	point_mutation	1448	1448	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K199152	.	engineered_tag	1713	1737	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K199152	.	ribosome_entry_site	939	950	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0462
BBa_K199152	.	conserved_region	943	946	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K199152	.	terminator	1834	1874	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0462
BBa_K199152	.	polyA_site	1861	1874	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K199152	.	engineered_region	1834	1874	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K199152	.	stem_loop	1841	1860	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K199152	.	stop_codon	1867	1867	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K199152	.	promoter	876	929	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_K199103
BBa_K199152	.	operator	878	894	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_K199152	.	operator	901	917	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_K199152	.	promoter	918	923	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_K199152	.	engineered_region	876	929	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_K199152	.	promoter	895	900	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
>BBa_K199152
ttaagcaccggtggagtgacgaccttcagcacgttcgtactgttcaacgatggtgtagtcttcgttgtgggaggtgatg
tccagtttgatgtcggttttgtaagcacccggcagctgaaccggttttttagccatgtaggtggttttaacttcagcgt
cgtagtgaccaccgtctttcagtttcagacgcattttgatttcacctttcagagcaccgtcttccgggtacatacgttc
ggtggaagcttcccaacccatggtttttttctgcataaccggaccgtcggacgggaagttggtaccacgcagtttaact
ttgtagatgaactcaccgtcttgcagggaggagtcctgggtaacggtaacaacaccaccgtcttcgaagttcataacac
gttcccatttgaaaccttccgggaaggacagtttcaggtagtccgggatgtcagccgggtgtttaacgtaagctttgga
accgtactggaactgcggggacaggatgtcccaagcgaacggcagcggaccacctttggtaactttcagtttagcggtc
tgggtaccttcgtacggacgaccttcaccttcaccttcgatttcgaactcgtgaccgttaacggaaccttccatacgaa
ctttgaaacgcatgaactctttgataacgtcttcggaggaagccattactagagtttctcctctttaattactagagac
ctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagtcacactggctcaccttc
gggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattatttta
ctagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggaga
aatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatga
tattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattct
atggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataa
aatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaa
taaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggct
aacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgta
tgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacga
tttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggt
tgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtattt
ctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcact
actagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtga
acgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata