LOCUS       BBa_S04429               867 bp    DNA     linear       18-Nov-2024
DEFINITION  K199027:B0014
ACCESSION   BBa_S04429
VERSION     BBa_S04429.1  
FEATURES             Location/Qualifiers
     CDS             1..678
                     /label=mRFP reverse
     primer_bind     659..678
                     /label=Spe mRFP F
     primer_bind     1..20
                     /label=Xba mRFP R
     misc_feature    1..678
                     /label=BBa_J31008
     RBS             687..701
                     /label=Reverse RBS
     RBS             687..701
                     /label=BBa_J44001
     misc_feature    710..729
                     /label=LuxR/HSL
     promoter        729..734
                     /label=-35
     promoter        751..756
                     /label=-10
     misc_feature    762..762
                     /label=start
     misc_feature    710..764
                     /label=BBa_R0062
     promoter        710..764
                     /label=BBa_R0062
     stem_loop       780..799
                     /label=T7 TE
     misc_feature    806..806
                     /label=stop
     polyA_site      800..813
                     /label=polya
     misc_feature    773..813
                     /label=BBa_B0012
     terminator      773..813
                     /label=BBa_B0012
     stem_loop       834..856
                     /label=stem_loop
     misc_feature    822..867
                     /label=BBa_B0011
     terminator      822..867
                     /label=BBa_B0011
ORIGIN
        1 ttaagcaccg gtggagtgac gaccttcagc acgttcgtac tgttcaacga tggtgtagtc
       61 ttcgttgtgg gaggtgatgt ccagtttgat gtcggttttg taagcacccg gcagctgaac
      121 cggtttttta gccatgtagg tggttttaac ttcagcgtcg tagtgaccac cgtctttcag
      181 tttcagacgc attttgattt cacctttcag agcaccgtct tccgggtaca tacgttcggt
      241 ggaagcttcc caacccatgg tttttttctg cataaccgga ccgtcggacg ggaagttggt
      301 accacgcagt ttaactttgt agatgaactc accgtcttgc agggaggagt cctgggtaac
      361 ggtaacaaca ccaccgtctt cgaagttcat aacacgttcc catttgaaac cttccgggaa
      421 ggacagtttc aggtagtccg ggatgtcagc cgggtgttta acgtaagctt tggaaccgta
      481 ctggaactgc ggggacagga tgtcccaagc gaacggcagc ggaccacctt tggtaacttt
      541 cagtttagcg gtctgggtac cttcgtacgg acgaccttca ccttcacctt cgatttcgaa
      601 ctcgtgaccg ttaacggaac cttccatacg aactttgaaa cgcatgaact ctttgataac
      661 gtcttcggag gaagccatta ctagagtttc tcctctttaa ttactagaga cctgtaggat
      721 cgtacaggtt tacgcaagaa aatggtttgt tatagtcgaa taaatactag agtcacactg
      781 gctcaccttc gggtgggcct ttctgcgttt atatactaga gagagaatat aaaaagccag
      841 attattaatc cggctttttt attattt
//
LOCUS       BBa_K199153              867 bp    DNA     linear       18-Nov-2024
DEFINITION  Measures the reverse activity of pLux in absence of LuxR
ACCESSION   BBa_K199153
VERSION     BBa_K199153.1  
FEATURES             Location/Qualifiers
     CDS             1..678
                     /label=mRFP reverse
     primer_bind     659..678
                     /label=Spe mRFP F
     primer_bind     1..20
                     /label=Xba mRFP R
     misc_feature    1..678
                     /label=BBa_J31008
     RBS             687..701
                     /label=Reverse RBS
     RBS             687..701
                     /label=BBa_J44001
     misc_feature    710..729
                     /label=LuxR/HSL
     promoter        729..734
                     /label=-35
     promoter        751..756
                     /label=-10
     misc_feature    762..762
                     /label=start
     misc_feature    710..764
                     /label=BBa_R0062
     promoter        710..764
                     /label=BBa_R0062
     stem_loop       780..799
                     /label=T7 TE
     misc_feature    806..806
                     /label=stop
     polyA_site      800..813
                     /label=polya
     misc_feature    773..813
                     /label=BBa_B0012
     terminator      773..813
                     /label=BBa_B0012
     stem_loop       834..856
                     /label=stem_loop
     misc_feature    822..867
                     /label=BBa_B0011
     terminator      822..867
                     /label=BBa_B0011
ORIGIN
        1 ttaagcaccg gtggagtgac gaccttcagc acgttcgtac tgttcaacga tggtgtagtc
       61 ttcgttgtgg gaggtgatgt ccagtttgat gtcggttttg taagcacccg gcagctgaac
      121 cggtttttta gccatgtagg tggttttaac ttcagcgtcg tagtgaccac cgtctttcag
      181 tttcagacgc attttgattt cacctttcag agcaccgtct tccgggtaca tacgttcggt
      241 ggaagcttcc caacccatgg tttttttctg cataaccgga ccgtcggacg ggaagttggt
      301 accacgcagt ttaactttgt agatgaactc accgtcttgc agggaggagt cctgggtaac
      361 ggtaacaaca ccaccgtctt cgaagttcat aacacgttcc catttgaaac cttccgggaa
      421 ggacagtttc aggtagtccg ggatgtcagc cgggtgttta acgtaagctt tggaaccgta
      481 ctggaactgc ggggacagga tgtcccaagc gaacggcagc ggaccacctt tggtaacttt
      541 cagtttagcg gtctgggtac cttcgtacgg acgaccttca ccttcacctt cgatttcgaa
      601 ctcgtgaccg ttaacggaac cttccatacg aactttgaaa cgcatgaact ctttgataac
      661 gtcttcggag gaagccatta ctagagtttc tcctctttaa ttactagaga cctgtaggat
      721 cgtacaggtt tacgcaagaa aatggtttgt tatagtcgaa taaatactag agtcacactg
      781 gctcaccttc gggtgggcct ttctgcgttt atatactaga gagagaatat aaaaagccag
      841 attattaatc cggctttttt attattt
//