##gff-version 3 ##sequence-region BBa_S04429 1 867 ##sequence-region BBa_K199153 1 867 BBa_S04429 . terminator 773 813 . + 0 ID=BBa_B0012;Name=BBa_B0012 BBa_S04429 . polyA_site 800 813 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_S04429 . engineered_region 773 813 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_S04429 . stem_loop 780 799 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_S04429 . stop_codon 806 806 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_S04429 . engineered_region 1 678 . + 0 ID=BBa_J31008;Name=BBa_J31008 BBa_S04429 . CDS 1 678 . + 0 ID=annotation1911761;Name=mRFP reverse;Parent=BBa_J31008 BBa_S04429 . primer_binding_site 659 678 . + 0 ID=annotation1911764;Name=Spe mRFP F;Parent=BBa_J31008 BBa_S04429 . primer_binding_site 1 20 . + 0 ID=annotation1911765;Name=Xba mRFP R;Parent=BBa_J31008 BBa_S04429 . ribosome_entry_site 687 701 . + 0 ID=BBa_J44001;Name=BBa_J44001 BBa_S04429 . ribosome_entry_site 687 701 . + 0 ID=annotation1893199;Name=Reverse RBS;Parent=BBa_J44001 BBa_S04429 . terminator 822 867 . + 0 ID=BBa_B0011;Name=BBa_B0011 BBa_S04429 . stem_loop 834 856 . + 0 ID=annotation1683;Name=stem_loop;Parent=BBa_B0011 BBa_S04429 . engineered_region 822 867 . + 0 ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011 BBa_S04429 . promoter 710 764 . + 0 ID=BBa_R0062;Name=BBa_R0062 BBa_S04429 . non_covalent_binding_site 710 729 . + 0 ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062 BBa_S04429 . promoter 751 756 . + 0 ID=annotation2047;Name=-10;Parent=BBa_R0062 BBa_S04429 . promoter 729 734 . + 0 ID=annotation2046;Name=-35;Parent=BBa_R0062 BBa_S04429 . start_codon 762 762 . + 0 ID=annotation2048;Name=start;Parent=BBa_R0062 BBa_S04429 . engineered_region 710 764 . + 0 ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062 BBa_K199153 . promoter 710 764 . + 0 ID=BBa_R0062;Name=BBa_R0062 BBa_K199153 . non_covalent_binding_site 710 729 . + 0 ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062 BBa_K199153 . promoter 751 756 . + 0 ID=annotation2047;Name=-10;Parent=BBa_R0062 BBa_K199153 . promoter 729 734 . + 0 ID=annotation2046;Name=-35;Parent=BBa_R0062 BBa_K199153 . start_codon 762 762 . + 0 ID=annotation2048;Name=start;Parent=BBa_R0062 BBa_K199153 . engineered_region 710 764 . + 0 ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062 BBa_K199153 . ribosome_entry_site 687 701 . + 0 ID=BBa_J44001;Name=BBa_J44001 BBa_K199153 . ribosome_entry_site 687 701 . + 0 ID=annotation1893199;Name=Reverse RBS;Parent=BBa_J44001 BBa_K199153 . engineered_region 1 678 . + 0 ID=BBa_J31008;Name=BBa_J31008 BBa_K199153 . CDS 1 678 . + 0 ID=annotation1911761;Name=mRFP reverse;Parent=BBa_J31008 BBa_K199153 . primer_binding_site 659 678 . + 0 ID=annotation1911764;Name=Spe mRFP F;Parent=BBa_J31008 BBa_K199153 . primer_binding_site 1 20 . + 0 ID=annotation1911765;Name=Xba mRFP R;Parent=BBa_J31008 BBa_K199153 . terminator 822 867 . + 0 ID=BBa_B0011;Name=BBa_B0011 BBa_K199153 . stem_loop 834 856 . + 0 ID=annotation1683;Name=stem_loop;Parent=BBa_B0011 BBa_K199153 . engineered_region 822 867 . + 0 ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011 BBa_K199153 . terminator 773 813 . + 0 ID=BBa_B0012;Name=BBa_B0012 BBa_K199153 . polyA_site 800 813 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_K199153 . engineered_region 773 813 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_K199153 . stem_loop 780 799 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_K199153 . stop_codon 806 806 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 >BBa_S04429 ttaagcaccggtggagtgacgaccttcagcacgttcgtactgttcaacgatggtgtagtcttcgttgtgggaggtgatg tccagtttgatgtcggttttgtaagcacccggcagctgaaccggttttttagccatgtaggtggttttaacttcagcgt cgtagtgaccaccgtctttcagtttcagacgcattttgatttcacctttcagagcaccgtcttccgggtacatacgttc ggtggaagcttcccaacccatggtttttttctgcataaccggaccgtcggacgggaagttggtaccacgcagtttaact ttgtagatgaactcaccgtcttgcagggaggagtcctgggtaacggtaacaacaccaccgtcttcgaagttcataacac gttcccatttgaaaccttccgggaaggacagtttcaggtagtccgggatgtcagccgggtgtttaacgtaagctttgga accgtactggaactgcggggacaggatgtcccaagcgaacggcagcggaccacctttggtaactttcagtttagcggtc tgggtaccttcgtacggacgaccttcaccttcaccttcgatttcgaactcgtgaccgttaacggaaccttccatacgaa ctttgaaacgcatgaactctttgataacgtcttcggaggaagccattactagagtttctcctctttaattactagagac ctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagtcacactggctcaccttc gggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt >BBa_K199153 ttaagcaccggtggagtgacgaccttcagcacgttcgtactgttcaacgatggtgtagtcttcgttgtgggaggtgatg tccagtttgatgtcggttttgtaagcacccggcagctgaaccggttttttagccatgtaggtggttttaacttcagcgt cgtagtgaccaccgtctttcagtttcagacgcattttgatttcacctttcagagcaccgtcttccgggtacatacgttc ggtggaagcttcccaacccatggtttttttctgcataaccggaccgtcggacgggaagttggtaccacgcagtttaact ttgtagatgaactcaccgtcttgcagggaggagtcctgggtaacggtaacaacaccaccgtcttcgaagttcataacac gttcccatttgaaaccttccgggaaggacagtttcaggtagtccgggatgtcagccgggtgtttaacgtaagctttgga accgtactggaactgcggggacaggatgtcccaagcgaacggcagcggaccacctttggtaactttcagtttagcggtc tgggtaccttcgtacggacgaccttcaccttcaccttcgatttcgaactcgtgaccgttaacggaaccttccatacgaa ctttgaaacgcatgaactctttgataacgtcttcggaggaagccattactagagtttctcctctttaattactagagac ctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagtcacactggctcaccttc gggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt