BBa_B0011
1
BBa_B0011
LuxICDABEG (+/-)
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>.
Released HQ 2013
Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.</p>
false
false
_1_
0
24
7
In stock
false
<P> <P>In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.<P>
true
Reshma Shetty
annotation7019
1
BBa_B0011
range7019
1
1
46
annotation1683
1
stem_loop
range1683
1
13
35
BBa_J44001
1
BBa_J44001
Reverse RBS (RBS<sub>rev</sub>) -- corresponds to BBa_B0030
2006-08-01T11:00:00Z
2015-08-31T04:08:48Z
Cloned from synthetic oligos
Released HQ 2013
Reverse version of RBS BBa_B0030
false
false
_61_71_
0
606
61
In stock
true
Repeats in oligos caused unusual products during cloning
true
Todd Eckdahl
annotation1893199
1
Reverse RBS
range1893199
1
1
15
BBa_S04429
1
BBa_S04429
K199027:B0014
2010-05-06T11:00:00Z
2015-05-08T01:14:40Z
false
true
_61_
0
201
61
Not in stock
false
false
Malcolm Campbell
component2068804
1
BBa_R0062
component2068802
1
BBa_J44001
component2068812
1
BBa_B0011
component2068800
1
BBa_J31008
component2068808
1
BBa_B0012
annotation2068808
1
BBa_B0012
range2068808
1
773
813
annotation2068812
1
BBa_B0011
range2068812
1
822
867
annotation2068804
1
BBa_R0062
range2068804
1
710
764
annotation2068802
1
BBa_J44001
range2068802
1
687
701
annotation2068800
1
BBa_J31008
range2068800
1
1
678
BBa_R0062
1
lux pR
Promoter (luxR & HSL regulated -- lux pR)
2003-01-31T12:00:00Z
2015-05-08T01:14:15Z
<em>V. fischeri</em>
Released HQ 2013
Promoter activated by LuxR in concert with HSL</p> <p>The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the LuxR activator protein complexed with the autoinducer, 3-oxo-hexanoyl-HSL. Two molecules of LuxR protein form a complex with two molecules of the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
false
true
_1_
0
24
7
In stock
false
<P> <P>This promoter is based on the <em>Vibrio fischeri </em>quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts <bb_part>BBa_R0062</bb_part> and <bb_part>BBa_R0063</bb_part> were derived is shown in the picture below. <p><img src="<bb_file>Image1.gif</bb_file>" width="614" height="362"><P>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
annotation2046
1
-35
range2046
1
20
25
annotation2045
1
LuxR/HSL
range2045
1
1
20
annotation2047
1
-10
range2047
1
42
47
annotation7070
1
BBa_R0062
range7070
1
1
55
annotation2048
1
start
range2048
1
53
53
BBa_K199153
1
BBa_K199153
Measures the reverse activity of pLux in absence of LuxR
2010-05-06T11:00:00Z
2015-05-08T01:11:20Z
This part was built from existing intermediate parts: RFPrev-RBSrev-pLux-TT and pLac-RBS
Measures the reverse activity of pLux in absence of LuxR
true
false
_48_
0
201
61
Discontinued
false
n/a
false
Malcolm Campbell
component2068969
1
BBa_B0012
component2068961
1
BBa_J31008
component2068965
1
BBa_R0062
component2068963
1
BBa_J44001
component2068973
1
BBa_B0011
annotation2068963
1
BBa_J44001
range2068963
1
687
701
annotation2068961
1
BBa_J31008
range2068961
1
1
678
annotation2068965
1
BBa_R0062
range2068965
1
710
764
annotation2068969
1
BBa_B0012
range2068969
1
773
813
annotation2068973
1
BBa_B0011
range2068973
1
822
867
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1690
1
polya
range1690
1
28
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1687
1
stop
range1687
1
34
34
BBa_J31008
1
mRFPr
RFP reverse
2007-02-15T12:00:00Z
2015-08-31T04:08:45Z
RFP reverse was PCR amplified from mRFP (BBaE1010).
The mRFP coding sequence in the reverse orientation. This part requires a reverse RBS and reverse promoter to be expressed
false
false
_61_
0
1144
61
It's complicated
false
RFP reverse was PCR amplified from mRFP (BBaE1010) using the following primers:
5'-atgcactagtatggcttcctccgaagacgt "Spe mRFP F"
5'-gcattctagattaagcaccggtggagtgac "Xba mRFP R"
false
Karmella Haynes
annotation1911764
1
Spe mRFP F
range1911764
1
659
678
annotation1911761
1
mRFP reverse
range1911761
1
1
678
annotation1911765
1
Xba mRFP R
range1911765
1
1
20
BBa_K199153_sequence
1
ttaagcaccggtggagtgacgaccttcagcacgttcgtactgttcaacgatggtgtagtcttcgttgtgggaggtgatgtccagtttgatgtcggttttgtaagcacccggcagctgaaccggttttttagccatgtaggtggttttaacttcagcgtcgtagtgaccaccgtctttcagtttcagacgcattttgatttcacctttcagagcaccgtcttccgggtacatacgttcggtggaagcttcccaacccatggtttttttctgcataaccggaccgtcggacgggaagttggtaccacgcagtttaactttgtagatgaactcaccgtcttgcagggaggagtcctgggtaacggtaacaacaccaccgtcttcgaagttcataacacgttcccatttgaaaccttccgggaaggacagtttcaggtagtccgggatgtcagccgggtgtttaacgtaagctttggaaccgtactggaactgcggggacaggatgtcccaagcgaacggcagcggaccacctttggtaactttcagtttagcggtctgggtaccttcgtacggacgaccttcaccttcaccttcgatttcgaactcgtgaccgttaacggaaccttccatacgaactttgaaacgcatgaactctttgataacgtcttcggaggaagccattactagagtttctcctctttaattactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
BBa_R0062_sequence
1
acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaa
BBa_J44001_sequence
1
tttctcctctttaat
BBa_J31008_sequence
1
ttaagcaccggtggagtgacgaccttcagcacgttcgtactgttcaacgatggtgtagtcttcgttgtgggaggtgatgtccagtttgatgtcggttttgtaagcacccggcagctgaaccggttttttagccatgtaggtggttttaacttcagcgtcgtagtgaccaccgtctttcagtttcagacgcattttgatttcacctttcagagcaccgtcttccgggtacatacgttcggtggaagcttcccaacccatggtttttttctgcataaccggaccgtcggacgggaagttggtaccacgcagtttaactttgtagatgaactcaccgtcttgcagggaggagtcctgggtaacggtaacaacaccaccgtcttcgaagttcataacacgttcccatttgaaaccttccgggaaggacagtttcaggtagtccgggatgtcagccgggtgtttaacgtaagctttggaaccgtactggaactgcggggacaggatgtcccaagcgaacggcagcggaccacctttggtaactttcagtttagcggtctgggtaccttcgtacggacgaccttcaccttcaccttcgatttcgaactcgtgaccgttaacggaaccttccatacgaactttgaaacgcatgaactctttgataacgtcttcggaggaagccat
BBa_B0011_sequence
1
agagaatataaaaagccagattattaatccggcttttttattattt
BBa_S04429_sequence
1
ttaagcaccggtggagtgacgaccttcagcacgttcgtactgttcaacgatggtgtagtcttcgttgtgggaggtgatgtccagtttgatgtcggttttgtaagcacccggcagctgaaccggttttttagccatgtaggtggttttaacttcagcgtcgtagtgaccaccgtctttcagtttcagacgcattttgatttcacctttcagagcaccgtcttccgggtacatacgttcggtggaagcttcccaacccatggtttttttctgcataaccggaccgtcggacgggaagttggtaccacgcagtttaactttgtagatgaactcaccgtcttgcagggaggagtcctgggtaacggtaacaacaccaccgtcttcgaagttcataacacgttcccatttgaaaccttccgggaaggacagtttcaggtagtccgggatgtcagccgggtgtttaacgtaagctttggaaccgtactggaactgcggggacaggatgtcccaagcgaacggcagcggaccacctttggtaactttcagtttagcggtctgggtaccttcgtacggacgaccttcaccttcaccttcgatttcgaactcgtgaccgttaacggaaccttccatacgaactttgaaacgcatgaactctttgataacgtcttcggaggaagccattactagagtttctcctctttaattactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z