BBa_K199153
type: ComponentDefinition
persistent identity: BBa K199153
display id: BBa_K199153
version: 1
was generated by: iGEM to SBOL conversion
description: Measures the reverse activity of pLux in absence of LuxR
created: 2010-05-06T11:00:00Z
modified: 2015-05-08T01:11:20Z
mutable provenance: This part was built from existing intermediate parts: RFPrev-RBSrev-pLux-TT and pLac-RBS
owned by: james
owned by: myers
mutable description: Measures the reverse activity of pLux in absence of LuxR
discontinued: true
dominant: false
experience: experience/None
group u list: _48_
m user id: 0
owner id: 201
owning group id: 61
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable notes: n/a
star: false
is replaced by: BBa_S04429
creator: Malcolm Campbell
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_J31008
component: BBa_R0062
component: BBa_J44001
component: BBa_B0011
component: BBa_B0012
sequence annotation: BBa_J31008
sequence annotation: BBa_J44001
sequence annotation: BBa_R0062
sequence annotation: BBa_B0012
sequence annotation: BBa_B0011
sequence: https://synbiohub.org/public/igem/BBa_K199153_sequence/1

BBa_J31008
type: Component
persistent identity: component2068961
display id: component2068961
version: 1
top level: BBa_K199153
definition: mRFPr
access: public

BBa_R0062
type: Component
persistent identity: component2068965
display id: component2068965
version: 1
top level: BBa_K199153
definition: lux pR
access: public

BBa_J44001
type: Component
persistent identity: component2068963
display id: component2068963
version: 1
top level: BBa_K199153
definition: BBa_J44001
access: public

BBa_B0011
type: Component
persistent identity: component2068973
display id: component2068973
version: 1
top level: BBa_K199153
definition: BBa_B0011
access: public

BBa_B0012
type: Component
persistent identity: component2068969
display id: component2068969
version: 1
top level: BBa_K199153
definition: BBa_B0012
access: public

BBa_J31008
type: SequenceAnnotation
persistent identity: annotation2068961
display id: annotation2068961
version: 1
top level: BBa_K199153
location: https://synbiohub.org/public/igem/BBa_K199153/annotation2068961/range2068961/1
component: BBa_J31008

https://synbiohub.org/public/igem/BBa_K199153/annotation2068961/range2068961/1
type: Range
persistent identity: range2068961
display id: range2068961
version: 1
top level: BBa_K199153
direction: direction/reverse
start: 1
end: 678
orientation: inline

BBa_J44001
type: SequenceAnnotation
persistent identity: annotation2068963
display id: annotation2068963
version: 1
top level: BBa_K199153
location: https://synbiohub.org/public/igem/BBa_K199153/annotation2068963/range2068963/1
component: BBa_J44001

https://synbiohub.org/public/igem/BBa_K199153/annotation2068963/range2068963/1
type: Range
persistent identity: range2068963
display id: range2068963
version: 1
top level: BBa_K199153
direction: direction/reverse
start: 687
end: 701
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2068965
display id: annotation2068965
version: 1
top level: BBa_K199153
location: https://synbiohub.org/public/igem/BBa_K199153/annotation2068965/range2068965/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K199153/annotation2068965/range2068965/1
type: Range
persistent identity: range2068965
display id: range2068965
version: 1
top level: BBa_K199153
direction: direction/forward
start: 710
end: 764
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2068969
display id: annotation2068969
version: 1
top level: BBa_K199153
location: https://synbiohub.org/public/igem/BBa_K199153/annotation2068969/range2068969/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K199153/annotation2068969/range2068969/1
type: Range
persistent identity: range2068969
display id: range2068969
version: 1
top level: BBa_K199153
direction: direction/forward
start: 773
end: 813
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation2068973
display id: annotation2068973
version: 1
top level: BBa_K199153
location: https://synbiohub.org/public/igem/BBa_K199153/annotation2068973/range2068973/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_K199153/annotation2068973/range2068973/1
type: Range
persistent identity: range2068973
display id: range2068973
version: 1
top level: BBa_K199153
direction: direction/forward
start: 822
end: 867
orientation: inline

BBa_S04429
type: ComponentDefinition
persistent identity: BBa S04429
display id: BBa_S04429
version: 1
was generated by: iGEM to SBOL conversion
description: K199027:B0014
created: 2010-05-06T11:00:00Z
modified: 2015-05-08T01:14:40Z
owned by: james
owned by: myers
discontinued: false
dominant: true
group u list: _61_
m user id: 0
owner id: 201
owning group id: 61
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Malcolm Campbell
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_J44001
component: BBa_J31008
component: BBa_B0012
component: BBa_R0062
component: BBa_B0011
sequence annotation: BBa_J31008
sequence annotation: BBa_J44001
sequence annotation: BBa_R0062
sequence annotation: BBa_B0012
sequence annotation: BBa_B0011
sequence: https://synbiohub.org/public/igem/BBa_S04429_sequence/1

BBa_J44001
type: Component
persistent identity: component2068802
display id: component2068802
version: 1
top level: BBa_S04429
definition: BBa_J44001
access: public

BBa_J31008
type: Component
persistent identity: component2068800
display id: component2068800
version: 1
top level: BBa_S04429
definition: mRFPr
access: public

BBa_B0012
type: Component
persistent identity: component2068808
display id: component2068808
version: 1
top level: BBa_S04429
definition: BBa_B0012
access: public

BBa_R0062
type: Component
persistent identity: component2068804
display id: component2068804
version: 1
top level: BBa_S04429
definition: lux pR
access: public

BBa_B0011
type: Component
persistent identity: component2068812
display id: component2068812
version: 1
top level: BBa_S04429
definition: BBa_B0011
access: public

BBa_J31008
type: SequenceAnnotation
persistent identity: annotation2068800
display id: annotation2068800
version: 1
top level: BBa_S04429
location: https://synbiohub.org/public/igem/BBa_S04429/annotation2068800/range2068800/1
component: BBa_J31008

https://synbiohub.org/public/igem/BBa_S04429/annotation2068800/range2068800/1
type: Range
persistent identity: range2068800
display id: range2068800
version: 1
top level: BBa_S04429
direction: direction/reverse
start: 1
end: 678
orientation: inline

BBa_J44001
type: SequenceAnnotation
persistent identity: annotation2068802
display id: annotation2068802
version: 1
top level: BBa_S04429
location: https://synbiohub.org/public/igem/BBa_S04429/annotation2068802/range2068802/1
component: BBa_J44001

https://synbiohub.org/public/igem/BBa_S04429/annotation2068802/range2068802/1
type: Range
persistent identity: range2068802
display id: range2068802
version: 1
top level: BBa_S04429
direction: direction/reverse
start: 687
end: 701
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2068804
display id: annotation2068804
version: 1
top level: BBa_S04429
location: https://synbiohub.org/public/igem/BBa_S04429/annotation2068804/range2068804/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_S04429/annotation2068804/range2068804/1
type: Range
persistent identity: range2068804
display id: range2068804
version: 1
top level: BBa_S04429
direction: direction/forward
start: 710
end: 764
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2068808
display id: annotation2068808
version: 1
top level: BBa_S04429
location: https://synbiohub.org/public/igem/BBa_S04429/annotation2068808/range2068808/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_S04429/annotation2068808/range2068808/1
type: Range
persistent identity: range2068808
display id: range2068808
version: 1
top level: BBa_S04429
direction: direction/forward
start: 773
end: 813
orientation: inline

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation2068812
display id: annotation2068812
version: 1
top level: BBa_S04429
location: https://synbiohub.org/public/igem/BBa_S04429/annotation2068812/range2068812/1
component: BBa_B0011

https://synbiohub.org/public/igem/BBa_S04429/annotation2068812/range2068812/1
type: Range
persistent identity: range2068812
display id: range2068812
version: 1
top level: BBa_S04429
direction: direction/forward
start: 822
end: 867
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

lux pR
type: ComponentDefinition
persistent identity: BBa R0062
display id: BBa_R0062
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (luxR & HSL regulated -- lux pR)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: V. fischeri
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Promoter activated by LuxR in concert with HSL The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the LuxR activator protein complexed with the autoinducer, 3-oxo-hexanoyl-HSL. Two molecules of LuxR protein form a complex with two molecules of the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: This promoter is based on the Vibrio fischeri quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts <bb_part>BBa_R0062</bb_part> and <bb_part>BBa_R0063</bb_part> were derived is shown in the picture below. <img src="<bb_file>Image1.gif</bb_file>" width="614" height="362">
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: BBa_R0062
sequence annotation: LuxR/HSL
sequence annotation: -35
sequence annotation: -10
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0062_sequence/1

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation7070
display id: annotation7070
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation7070/range7070/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0062/annotation7070/range7070/1
type: Range
persistent identity: range7070
display id: range7070
version: 1
top level: lux pR
direction: direction/forward
start: 1
end: 55
orientation: inline

LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2045
display id: annotation2045
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2045/range2045/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0062/annotation2045/range2045/1
type: Range
persistent identity: range2045
display id: range2045
version: 1
top level: lux pR
direction: direction/forward
start: 1
end: 20
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2046
display id: annotation2046
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2046/range2046/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0062/annotation2046/range2046/1
type: Range
persistent identity: range2046
display id: range2046
version: 1
top level: lux pR
direction: direction/forward
start: 20
end: 25
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2047
display id: annotation2047
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2047/range2047/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0062/annotation2047/range2047/1
type: Range
persistent identity: range2047
display id: range2047
version: 1
top level: lux pR
direction: direction/forward
start: 42
end: 47
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2048
display id: annotation2048
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2048/range2048/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0062/annotation2048/range2048/1
type: Range
persistent identity: range2048
display id: range2048
version: 1
top level: lux pR
direction: direction/forward
start: 53
end: 53
orientation: inline

BBa_J44001
type: ComponentDefinition
persistent identity: BBa J44001
display id: BBa_J44001
version: 1
was generated by: iGEM to SBOL conversion
description: Reverse RBS (RBSrev) -- corresponds to BBa_B0030
created: 2006-08-01T11:00:00Z
modified: 2015-08-31T04:08:48Z
mutable provenance: Cloned from synthetic oligos
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Reverse version of RBS BBa_B0030
discontinued: false
dominant: false
experience: experience/Works
group u list: _61_71_
m user id: 0
owner id: 606
owning group id: 61
sample status: In stock
status: status/Available
bookmark: true
mutable notes: Repeats in oligos caused unusual products during cloning
star: true
creator: Todd Eckdahl
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: Reverse RBS
sequence: https://synbiohub.org/public/igem/BBa_J44001_sequence/1

Reverse RBS
type: SequenceAnnotation
persistent identity: annotation1893199
display id: annotation1893199
version: 1
top level: BBa_J44001
location: https://synbiohub.org/public/igem/BBa_J44001/annotation1893199/range1893199/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_J44001/annotation1893199/range1893199/1
type: Range
persistent identity: range1893199
display id: range1893199
version: 1
top level: BBa_J44001
direction: direction/reverse
start: 1
end: 15
orientation: inline

mRFPr
type: ComponentDefinition
persistent identity: BBa J31008
display id: BBa_J31008
version: 1
was generated by: iGEM to SBOL conversion
description: RFP reverse
created: 2007-02-15T12:00:00Z
modified: 2015-08-31T04:08:45Z
mutable provenance: RFP reverse was PCR amplified from mRFP (BBaE1010).
owned by: james
owned by: myers
mutable description: The mRFP coding sequence in the reverse orientation. This part requires a reverse RBS and reverse promoter to be expressed
discontinued: false
dominant: false
experience: experience/Works
group u list: _61_
m user id: 0
owner id: 1144
owning group id: 61
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: RFP reverse was PCR amplified from mRFP (BBaE1010) using the following primers: 5'-atgcactagtatggcttcctccgaagacgt "Spe mRFP F" 5'-gcattctagattaagcaccggtggagtgac "Xba mRFP R"
star: false
creator: Karmella Haynes
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: mRFP reverse
sequence annotation: Xba mRFP R
sequence annotation: Spe mRFP F
sequence: https://synbiohub.org/public/igem/BBa_J31008_sequence/1

mRFP reverse
type: SequenceAnnotation
persistent identity: annotation1911761
display id: annotation1911761
version: 1
top level: mRFPr
location: https://synbiohub.org/public/igem/BBa_J31008/annotation1911761/range1911761/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_J31008/annotation1911761/range1911761/1
type: Range
persistent identity: range1911761
display id: range1911761
version: 1
top level: mRFPr
direction: direction/reverse
start: 1
end: 678
orientation: inline

Xba mRFP R
type: SequenceAnnotation
persistent identity: annotation1911765
display id: annotation1911765
version: 1
top level: mRFPr
location: https://synbiohub.org/public/igem/BBa_J31008/annotation1911765/range1911765/1
role: SO:0005850
role: feature/primer binding

https://synbiohub.org/public/igem/BBa_J31008/annotation1911765/range1911765/1
type: Range
persistent identity: range1911765
display id: range1911765
version: 1
top level: mRFPr
direction: direction/forward
start: 1
end: 20
orientation: inline

Spe mRFP F
type: SequenceAnnotation
persistent identity: annotation1911764
display id: annotation1911764
version: 1
top level: mRFPr
location: https://synbiohub.org/public/igem/BBa_J31008/annotation1911764/range1911764/1
role: SO:0005850
role: feature/primer binding

https://synbiohub.org/public/igem/BBa_J31008/annotation1911764/range1911764/1
type: Range
persistent identity: range1911764
display id: range1911764
version: 1
top level: mRFPr
direction: direction/reverse
start: 659
end: 678
orientation: inline

BBa_B0011
type: ComponentDefinition
persistent identity: BBa B0011
display id: BBa_B0011
version: 1
was generated by: iGEM to SBOL conversion
description: LuxICDABEG (+/-)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A->G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0011
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0011_sequence/1

BBa_B0011
type: SequenceAnnotation
persistent identity: annotation7019
display id: annotation7019
version: 1
top level: BBa_B0011
location: https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0011/annotation7019/range7019/1
type: Range
persistent identity: range7019
display id: range7019
version: 1
top level: BBa_B0011
direction: direction/forward
start: 1
end: 46
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation1683
display id: annotation1683
version: 1
top level: BBa_B0011
location: https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0011/annotation1683/range1683/1
type: Range
persistent identity: range1683
display id: range1683
version: 1
top level: BBa_B0011
direction: direction/forward
start: 13
end: 35
orientation: inline

https://synbiohub.org/public/igem/BBa_K199153_sequence/1
type: Sequence
persistent identity: BBa K199153 sequence
display id: BBa_K199153_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttaagcaccggtggagtgacgaccttcagcacgttcgtactgttcaacgatggtgtagtcttcgttgtgggaggtgatgtccagtttgatgtcggttttgtaagcacccggcagctgaaccggttttttagccatgtaggtggttttaacttcagcgtcgtagtgaccaccgtctttcagtttcagacgcattttgatttcacctttcagagcaccgtcttccgggtacatacgttcggtggaagcttcccaacccatggtttttttctgcataaccggaccgtcggacgggaagttggtaccacgcagtttaactttgtagatgaactcaccgtcttgcagggaggagtcctgggtaacggtaacaacaccaccgtcttcgaagttcataacacgttcccatttgaaaccttccgggaaggacagtttcaggtagtccgggatgtcagccgggtgtttaacgtaagctttggaaccgtactggaactgcggggacaggatgtcccaagcgaacggcagcggaccacctttggtaactttcagtttagcggtctgggtaccttcgtacggacgaccttcaccttcaccttcgatttcgaactcgtgaccgttaacggaaccttccatacgaactttgaaacgcatgaactctttgataacgtcttcggaggaagccattactagagtttctcctctttaattactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S04429_sequence/1
type: Sequence
persistent identity: BBa S04429 sequence
display id: BBa_S04429_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttaagcaccggtggagtgacgaccttcagcacgttcgtactgttcaacgatggtgtagtcttcgttgtgggaggtgatgtccagtttgatgtcggttttgtaagcacccggcagctgaaccggttttttagccatgtaggtggttttaacttcagcgtcgtagtgaccaccgtctttcagtttcagacgcattttgatttcacctttcagagcaccgtcttccgggtacatacgttcggtggaagcttcccaacccatggtttttttctgcataaccggaccgtcggacgggaagttggtaccacgcagtttaactttgtagatgaactcaccgtcttgcagggaggagtcctgggtaacggtaacaacaccaccgtcttcgaagttcataacacgttcccatttgaaaccttccgggaaggacagtttcaggtagtccgggatgtcagccgggtgtttaacgtaagctttggaaccgtactggaactgcggggacaggatgtcccaagcgaacggcagcggaccacctttggtaactttcagtttagcggtctgggtaccttcgtacggacgaccttcaccttcaccttcgatttcgaactcgtgaccgttaacggaaccttccatacgaactttgaaacgcatgaactctttgataacgtcttcggaggaagccattactagagtttctcctctttaattactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0062_sequence/1
type: Sequence
persistent identity: BBa R0062 sequence
display id: BBa_R0062_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J44001_sequence/1
type: Sequence
persistent identity: BBa J44001 sequence
display id: BBa_J44001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttctcctctttaat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0011_sequence/1
type: Sequence
persistent identity: BBa B0011 sequence
display id: BBa_B0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agagaatataaaaagccagattattaatccggcttttttattattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J31008_sequence/1
type: Sequence
persistent identity: BBa J31008 sequence
display id: BBa_J31008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttaagcaccggtggagtgacgaccttcagcacgttcgtactgttcaacgatggtgtagtcttcgttgtgggaggtgatgtccagtttgatgtcggttttgtaagcacccggcagctgaaccggttttttagccatgtaggtggttttaacttcagcgtcgtagtgaccaccgtctttcagtttcagacgcattttgatttcacctttcagagcaccgtcttccgggtacatacgttcggtggaagcttcccaacccatggtttttttctgcataaccggaccgtcggacgggaagttggtaccacgcagtttaactttgtagatgaactcaccgtcttgcagggaggagtcctgggtaacggtaacaacaccaccgtcttcgaagttcataacacgttcccatttgaaaccttccgggaaggacagtttcaggtagtccgggatgtcagccgggtgtttaacgtaagctttggaaccgtactggaactgcggggacaggatgtcccaagcgaacggcagcggaccacctttggtaactttcagtttagcggtctgggtaccttcgtacggacgaccttcaccttcaccttcgatttcgaactcgtgaccgttaacggaaccttccatacgaactttgaaacgcatgaactctttgataacgtcttcggaggaagccat
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z