##gff-version 3
##sequence-region BBa_K199154 1 1095
BBa_K199154	.	ribosome_entry_site	1084	1095	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K199154	.	conserved_region	1088	1091	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K199154	.	engineered_region	1	678	.	+	0	ID=BBa_J31008;Name=BBa_J31008
BBa_K199154	.	primer_binding_site	659	678	.	+	0	ID=annotation1911764;Name=Spe mRFP F;Parent=BBa_J31008
BBa_K199154	.	CDS	1	678	.	+	0	ID=annotation1911761;Name=mRFP reverse;Parent=BBa_J31008
BBa_K199154	.	primer_binding_site	1	20	.	+	0	ID=annotation1911765;Name=Xba mRFP R;Parent=BBa_J31008
BBa_K199154	.	terminator	773	813	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K199154	.	polyA_site	800	813	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K199154	.	stem_loop	780	799	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K199154	.	engineered_region	773	813	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K199154	.	stop_codon	806	806	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K199154	.	promoter	876	1075	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_K199154	.	start_codon	1048	1048	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K199154	.	non_covalent_binding_site	964	1001	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K199154	.	engineered_region	876	1075	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K199154	.	promoter	1036	1041	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K199154	.	non_covalent_binding_site	1041	1075	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K199154	.	promoter	1012	1017	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K199154	.	CDS	876	963	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K199154	.	ribosome_entry_site	687	701	.	+	0	ID=BBa_J44001;Name=BBa_J44001
BBa_K199154	.	ribosome_entry_site	687	701	.	+	0	ID=annotation1893199;Name=Reverse RBS;Parent=BBa_J44001
BBa_K199154	.	terminator	822	867	.	+	0	ID=BBa_B0011;Name=BBa_B0011
BBa_K199154	.	engineered_region	822	867	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_K199154	.	stem_loop	834	856	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_K199154	.	promoter	710	764	.	+	0	ID=BBa_R0062;Name=BBa_R0062
BBa_K199154	.	promoter	729	734	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K199154	.	non_covalent_binding_site	710	729	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K199154	.	promoter	751	756	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K199154	.	engineered_region	710	764	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K199154	.	start_codon	762	762	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
>BBa_K199154
ttaagcaccggtggagtgacgaccttcagcacgttcgtactgttcaacgatggtgtagtcttcgttgtgggaggtgatg
tccagtttgatgtcggttttgtaagcacccggcagctgaaccggttttttagccatgtaggtggttttaacttcagcgt
cgtagtgaccaccgtctttcagtttcagacgcattttgatttcacctttcagagcaccgtcttccgggtacatacgttc
ggtggaagcttcccaacccatggtttttttctgcataaccggaccgtcggacgggaagttggtaccacgcagtttaact
ttgtagatgaactcaccgtcttgcagggaggagtcctgggtaacggtaacaacaccaccgtcttcgaagttcataacac
gttcccatttgaaaccttccgggaaggacagtttcaggtagtccgggatgtcagccgggtgtttaacgtaagctttgga
accgtactggaactgcggggacaggatgtcccaagcgaacggcagcggaccacctttggtaactttcagtttagcggtc
tgggtaccttcgtacggacgaccttcaccttcaccttcgatttcgaactcgtgaccgttaacggaaccttccatacgaa
ctttgaaacgcatgaactctttgataacgtcttcggaggaagccattactagagtttctcctctttaattactagagac
ctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagtcacactggctcaccttc
gggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattatttta
ctagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactg
gaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgctt
ccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaa