LOCUS       BBa_K199157             1353 bp    DNA     linear       19-Nov-2024
DEFINITION  pLac-RBS-CCACU-RFP-pLpp-CUAGU tRNA-pLpp-CCACU tRNA
ACCESSION   BBa_K199157
VERSION     BBa_K199157.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    230..234
                     /label=5 base pair addition
     misc_feature    650..655
                     /label=Nco1 restriction site
     misc_feature    913..937
                     /label=25 bp addition
     misc_feature    907..912
                     /label=Double Stop
     misc_feature    227..937
                     /label=BBa_K199005
     promoter        946..994
                     /label=BBa_K199106
     misc_feature    1003..1013
                     /label=5' Context
     misc_feature    1046..1054
                     /label=Anticodon Loop
     misc_feature    1106..1145
                     /label=3' Context
     misc_feature    1003..1145
                     /label=BBa_K199002
     promoter        1154..1202
                     /label=BBa_K199106
     misc_feature    1254..1262
                     /label=anticodon loop
     misc_feature    1211..1221
                     /label=5' context
     misc_feature    1314..1353
                     /label=3
     misc_feature    1211..1353
                     /label=BBa_K199028
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgc cactgcttcc
      241 tccgaagacg ttatcaaaga gttcatgcgt ttcaaagttc gtatggaagg ttccgttaac
      301 ggtcacgagt tcgaaatcga aggtgaaggt gaaggtcgtc cgtacgaagg tacccagacc
      361 gctaaactga aagttaccaa aggtggtccg ctgccgttcg cttgggacat cctgtccccg
      421 cagttccagt acggttccaa agcttacgtt aaacacccgg ctgacatccc ggactacctg
      481 aaactgtcct tcccggaagg tttcaaatgg gaacgtgtta tgaacttcga agacggtggt
      541 gttgttaccg ttacccagga ctcctccctg caagacggtg agttcatcta caaagttaaa
      601 ctgcgtggta ccaacttccc gtccgacggt ccggttatgc agaaaaaaac catgggttgg
      661 gaagcttcca ccgaacgtat gtacccggaa gacggtgctc tgaaaggtga aatcaaaatg
      721 cgtctgaaac tgaaagacgg tggtcactac gacgctgaag ttaaaaccac ctacatggct
      781 aaaaaaccgg ttcagctgcc gggtgcttac aaaaccgaca tcaaactgga catcacctcc
      841 cacaacgaag actacaccat cgttgaacag tacgaacgtg ctgaaggtcg tcactccacc
      901 ggtgcttaat aacgctgata gtgctagtgt agatcgctac tagagatcaa aaaaatattc
      961 tcaacataaa aaactttgtg taatacttgt aacgtactag agggatccaa ttcggagaga
     1021 tgccggagcg gctgaacgga ccggtctagt ggacaccgga gtaggggcaa ctctaccggg
     1081 ggttcaaatc cccctctctc cgccactgca tatccttagc gaaagctaag gatttttttt
     1141 aagcttacta gagatcaaaa aaatattctc aacataaaaa actttgtgta atacttgtaa
     1201 cgtactagag ggatccaatt cggagagatg ccggagcggc tgaacggacc ggtttgaccg
     1261 acaccggagt aggggcaact ctaccggggg ttcaaatccc cctctctccg ccactgcata
     1321 tccttagcga aagctaagga ttttttttaa gct
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