BBa_K199157
type: ComponentDefinition
persistent identity: BBa K199157
display id: BBa_K199157
version: 1
was generated by: iGEM to SBOL conversion
description: pLac-RBS-CCACU-RFP-pLpp-CUAGU tRNA-pLpp-CCACU tRNA
created: 2010-05-10T11:00:00Z
modified: 2015-05-08T01:11:20Z
mutable provenance: See individual tRNA pages and pLpp page.
owned by: james
owned by: myers
mutable description: This is a device that tests the production of the 2nd tRNA in a construct when each tRNA has its own promoter.
discontinued: false
dominant: false
experience: experience/Works
group u list: _295_
m user id: 0
owner id: 5562
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: n/a
star: false
creator: Mary Gearing
type: DnaRegion
role: SO:0000804
role: partType/Device
component: BBa_K199106
component: BBa_K199002
component: BBa_K199028
component: BBa_B0034
component: BBa_R0010
component: BBa_K199005
sequence annotation: BBa_R0010
sequence annotation: BBa_B0034
sequence annotation: BBa_K199005
sequence annotation: BBa_K199106
sequence annotation: BBa_K199002
sequence annotation: BBa_K199028
sequence: https://synbiohub.org/public/igem/BBa_K199157_sequence/1

BBa_K199106
type: Component
persistent identity: component2069145
display id: component2069145
version: 1
top level: BBa_K199157
definition: BBa_K199106
access: public

BBa_K199002
type: Component
persistent identity: component2069144
display id: component2069144
version: 1
top level: BBa_K199157
definition: BBa_K199002
access: public

BBa_K199028
type: Component
persistent identity: component2069149
display id: component2069149
version: 1
top level: BBa_K199157
definition: BBa_K199028
access: public

BBa_B0034
type: Component
persistent identity: component2069134
display id: component2069134
version: 1
top level: BBa_K199157
definition: BBa_B0034
access: public

BBa_R0010
type: Component
persistent identity: component2069126
display id: component2069126
version: 1
top level: BBa_K199157
definition: LacI
access: public

BBa_K199106
type: Component
persistent identity: component2069140
display id: component2069140
version: 1
top level: BBa_K199157
definition: BBa_K199106
access: public

BBa_K199005
type: Component
persistent identity: component2069139
display id: component2069139
version: 1
top level: BBa_K199157
definition: BBa_K199005
access: public

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2069126
display id: annotation2069126
version: 1
top level: BBa_K199157
location: https://synbiohub.org/public/igem/BBa_K199157/annotation2069126/range2069126/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K199157/annotation2069126/range2069126/1
type: Range
persistent identity: range2069126
display id: range2069126
version: 1
top level: BBa_K199157
direction: direction/forward
start: 1
end: 200
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2069134
display id: annotation2069134
version: 1
top level: BBa_K199157
location: https://synbiohub.org/public/igem/BBa_K199157/annotation2069134/range2069134/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K199157/annotation2069134/range2069134/1
type: Range
persistent identity: range2069134
display id: range2069134
version: 1
top level: BBa_K199157
direction: direction/reverse
start: 209
end: 220
orientation: inline

BBa_K199005
type: SequenceAnnotation
persistent identity: annotation2069139
display id: annotation2069139
version: 1
top level: BBa_K199157
location: https://synbiohub.org/public/igem/BBa_K199157/annotation2069139/range2069139/1
component: BBa_K199005

https://synbiohub.org/public/igem/BBa_K199157/annotation2069139/range2069139/1
type: Range
persistent identity: range2069139
display id: range2069139
version: 1
top level: BBa_K199157
direction: direction/reverse
start: 227
end: 937
orientation: inline

BBa_K199106
type: SequenceAnnotation
persistent identity: annotation2069140
display id: annotation2069140
version: 1
top level: BBa_K199157
location: https://synbiohub.org/public/igem/BBa_K199157/annotation2069140/range2069140/1
component: BBa_K199106

https://synbiohub.org/public/igem/BBa_K199157/annotation2069140/range2069140/1
type: Range
persistent identity: range2069140
display id: range2069140
version: 1
top level: BBa_K199157
direction: direction/reverse
start: 946
end: 994
orientation: inline

BBa_K199002
type: SequenceAnnotation
persistent identity: annotation2069144
display id: annotation2069144
version: 1
top level: BBa_K199157
location: https://synbiohub.org/public/igem/BBa_K199157/annotation2069144/range2069144/1
component: BBa_K199002

https://synbiohub.org/public/igem/BBa_K199157/annotation2069144/range2069144/1
type: Range
persistent identity: range2069144
display id: range2069144
version: 1
top level: BBa_K199157
direction: direction/reverse
start: 1003
end: 1145
orientation: inline

BBa_K199106
type: SequenceAnnotation
persistent identity: annotation2069145
display id: annotation2069145
version: 1
top level: BBa_K199157
location: https://synbiohub.org/public/igem/BBa_K199157/annotation2069145/range2069145/1
component: BBa_K199106

https://synbiohub.org/public/igem/BBa_K199157/annotation2069145/range2069145/1
type: Range
persistent identity: range2069145
display id: range2069145
version: 1
top level: BBa_K199157
direction: direction/reverse
start: 1154
end: 1202
orientation: inline

BBa_K199028
type: SequenceAnnotation
persistent identity: annotation2069149
display id: annotation2069149
version: 1
top level: BBa_K199157
location: https://synbiohub.org/public/igem/BBa_K199157/annotation2069149/range2069149/1
component: BBa_K199028

https://synbiohub.org/public/igem/BBa_K199157/annotation2069149/range2069149/1
type: Range
persistent identity: range2069149
display id: range2069149
version: 1
top level: BBa_K199157
direction: direction/reverse
start: 1211
end: 1353
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa R0010
display id: BBa_R0010
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lacI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
star: true
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_R0010
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0010_sequence/1

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1961221
display id: annotation1961221
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
type: Range
persistent identity: range1961221
display id: range1961221
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 88
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1961222
display id: annotation1961222
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
type: Range
persistent identity: range1961222
display id: range1961222
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 200
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1961223
display id: annotation1961223
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
type: Range
persistent identity: range1961223
display id: range1961223
version: 1
top level: LacI
direction: direction/forward
start: 89
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961224
display id: annotation1961224
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
type: Range
persistent identity: range1961224
display id: range1961224
version: 1
top level: LacI
direction: direction/forward
start: 137
end: 142
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961225
display id: annotation1961225
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
type: Range
persistent identity: range1961225
display id: range1961225
version: 1
top level: LacI
direction: direction/forward
start: 161
end: 166
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1961226
display id: annotation1961226
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
type: Range
persistent identity: range1961226
display id: range1961226
version: 1
top level: LacI
direction: direction/forward
start: 166
end: 200
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1961227
display id: annotation1961227
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
type: Range
persistent identity: range1961227
display id: range1961227
version: 1
top level: LacI
direction: direction/forward
start: 173
end: 173
orientation: inline

BBa_K199002
type: ComponentDefinition
persistent identity: BBa K199002
display id: BBa_K199002
version: 1
was generated by: iGEM to SBOL conversion
description: CCACU tRNA Suppressor (Produces Serine)
created: 2009-06-25T11:00:00Z
modified: 2015-05-08T01:11:18Z
mutable provenance: De Novo synthesis from oligos. Based on the paper by Anderson et al. (http://gcat.davidson.edu/GcatWiki/images/6/6f/AndersonSchultz2002ChemBiol.pdf)
owned by: james
owned by: myers
mutable description: This gene encodes for a 5 base pair tRNA suppressor that suppresses CCACU, which would normally cause a frame shift mutation.
discontinued: false
dominant: false
experience: experience/Works
group u list: _295_
m user id: 0
owner id: 5109
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: We used the 9 base anticodon loop that is complementary to the 5 base pair codon CCACU.
star: false
creator: Shamita Punjabi
type: DnaRegion
role: partType/RNA
role: SO:0000834
sequence annotation: 5' Context
sequence annotation: Anticodon Loop
sequence annotation: 3' Context
sequence: https://synbiohub.org/public/igem/BBa_K199002_sequence/1

5' Context
type: SequenceAnnotation
persistent identity: annotation2006707
display id: annotation2006707
version: 1
top level: BBa_K199002
location: https://synbiohub.org/public/igem/BBa_K199002/annotation2006707/range2006707/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K199002/annotation2006707/range2006707/1
type: Range
persistent identity: range2006707
display id: range2006707
version: 1
top level: BBa_K199002
direction: direction/forward
start: 1
end: 11
orientation: inline

Anticodon Loop
type: SequenceAnnotation
persistent identity: annotation2006708
display id: annotation2006708
version: 1
top level: BBa_K199002
location: https://synbiohub.org/public/igem/BBa_K199002/annotation2006708/range2006708/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K199002/annotation2006708/range2006708/1
type: Range
persistent identity: range2006708
display id: range2006708
version: 1
top level: BBa_K199002
direction: direction/forward
start: 44
end: 52
orientation: inline

3' Context
type: SequenceAnnotation
persistent identity: annotation2006709
display id: annotation2006709
version: 1
top level: BBa_K199002
location: https://synbiohub.org/public/igem/BBa_K199002/annotation2006709/range2006709/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K199002/annotation2006709/range2006709/1
type: Range
persistent identity: range2006709
display id: range2006709
version: 1
top level: BBa_K199002
direction: direction/forward
start: 104
end: 143
orientation: inline

BBa_K199005
type: ComponentDefinition
persistent identity: BBa K199005
display id: BBa_K199005
version: 1
was generated by: iGEM to SBOL conversion
description: RFP with CCACU Addition
created: 2009-06-25T11:00:00Z
modified: 2015-05-08T01:11:18Z
mutable provenance: The template of the gene comes from BioBrick part E1010. We designed a forward primer that included the first 20 nucleotides on the 5' end, and a reverse primer that included the last 20 nucleotides on the 3' end of the RFP gene.
owned by: james
owned by: myers
mutable description: This RFP reporter gene has a 5-base pair CCACU addition after the start codon and before the rest of the gene. This is used in conjunction with the CCACU tRNA (BioBrick part [http://partsregistry.org/Part:BBa_K199002 K199002]). This CCACU tRNA suppresses the frameshift caused by this 5-base pair addition in the reporter.
discontinued: false
dominant: false
experience: experience/Works
group u list: _295_
m user id: 0
owner id: 5120
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: We positioned the ATG before the 5-base codon and continued with the rest of the RFP gene. This way, the RBS wouldn't read the reporter gene without reading over the 5-base codon first. In order to ligate our 5-base pair addition at the desired position in the reporter gene, we utilized the restriction enzyme NcoI which cut at a single site 424 bp into the gene.
star: false
creator: Shamita Punjabi
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: 5 base pair addition
sequence annotation: Nco1 restriction site
sequence annotation: Double Stop
sequence annotation: 25 bp addition
sequence: https://synbiohub.org/public/igem/BBa_K199005_sequence/1

5 base pair addition
type: SequenceAnnotation
persistent identity: annotation2006731
display id: annotation2006731
version: 1
top level: BBa_K199005
location: https://synbiohub.org/public/igem/BBa_K199005/annotation2006731/range2006731/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K199005/annotation2006731/range2006731/1
type: Range
persistent identity: range2006731
display id: range2006731
version: 1
top level: BBa_K199005
direction: direction/forward
start: 4
end: 8
orientation: inline

Nco1 restriction site
type: SequenceAnnotation
persistent identity: annotation2006732
display id: annotation2006732
version: 1
top level: BBa_K199005
location: https://synbiohub.org/public/igem/BBa_K199005/annotation2006732/range2006732/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K199005/annotation2006732/range2006732/1
type: Range
persistent identity: range2006732
display id: range2006732
version: 1
top level: BBa_K199005
direction: direction/forward
start: 424
end: 429
orientation: inline

Double Stop
type: SequenceAnnotation
persistent identity: annotation2013714
display id: annotation2013714
version: 1
top level: BBa_K199005
location: https://synbiohub.org/public/igem/BBa_K199005/annotation2013714/range2013714/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K199005/annotation2013714/range2013714/1
type: Range
persistent identity: range2013714
display id: range2013714
version: 1
top level: BBa_K199005
direction: direction/forward
start: 681
end: 686
orientation: inline

25 bp addition
type: SequenceAnnotation
persistent identity: annotation2013325
display id: annotation2013325
version: 1
top level: BBa_K199005
location: https://synbiohub.org/public/igem/BBa_K199005/annotation2013325/range2013325/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K199005/annotation2013325/range2013325/1
type: Range
persistent identity: range2013325
display id: range2013325
version: 1
top level: BBa_K199005
direction: direction/forward
start: 687
end: 711
orientation: inline

BBa_K199028
type: ComponentDefinition
persistent identity: BBa K199028
display id: BBa_K199028
version: 1
was generated by: iGEM to SBOL conversion
description: CGGUC tRNA Suppressor (Produces Serine)
created: 2009-07-08T11:00:00Z
modified: 2015-05-08T01:11:18Z
mutable provenance: De Novo synthesis from oligos. Based on the paper by Anderson et al. (http://gcat.davidson.edu/GcatWiki/images/6/6f/AndersonSchultz2002ChemBiol.pdf)
owned by: james
owned by: myers
mutable description: This gene encodes for a 5 base pair tRNA suppressor that suppresses CGGUC, which would normally cause a frame shift mutation.
discontinued: false
dominant: false
experience: experience/Works
group u list: _295_
m user id: 0
owner id: 5117
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: We used the 9 base anticodon loop that is complementary to the 5 base pair codon CGGUC.
star: false
creator: Alyndria Thompson
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: 5' context
sequence annotation: anticodon loop
sequence annotation: 3
sequence: https://synbiohub.org/public/igem/BBa_K199028_sequence/1

5' context
type: SequenceAnnotation
persistent identity: annotation2010431
display id: annotation2010431
version: 1
top level: BBa_K199028
location: https://synbiohub.org/public/igem/BBa_K199028/annotation2010431/range2010431/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K199028/annotation2010431/range2010431/1
type: Range
persistent identity: range2010431
display id: range2010431
version: 1
top level: BBa_K199028
direction: direction/forward
start: 1
end: 11
orientation: inline

anticodon loop
type: SequenceAnnotation
persistent identity: annotation2010430
display id: annotation2010430
version: 1
top level: BBa_K199028
location: https://synbiohub.org/public/igem/BBa_K199028/annotation2010430/range2010430/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K199028/annotation2010430/range2010430/1
type: Range
persistent identity: range2010430
display id: range2010430
version: 1
top level: BBa_K199028
direction: direction/forward
start: 44
end: 52
orientation: inline

3
type: SequenceAnnotation
persistent identity: annotation2010432
display id: annotation2010432
version: 1
top level: BBa_K199028
location: https://synbiohub.org/public/igem/BBa_K199028/annotation2010432/range2010432/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K199028/annotation2010432/range2010432/1
type: Range
persistent identity: range2010432
display id: range2010432
version: 1
top level: BBa_K199028
direction: direction/forward
start: 104
end: 143
orientation: inline

BBa_K199106
type: ComponentDefinition
persistent identity: BBa K199106
display id: BBa_K199106
version: 1
was generated by: iGEM to SBOL conversion
description: pLpp promoter
created: 2009-11-18T12:00:00Z
modified: 2015-05-08T01:11:20Z
mutable provenance: The sequence matches that naturally found in E. coli.
owned by: james
owned by: myers
mutable description: The lpp promoter is one of the strongest promoters in E. coli.
discontinued: false
dominant: false
experience: experience/Works
group u list: _295_
m user id: 0
owner id: 5562
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: n/a
star: false
creator: Mary Gearing
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K199106_sequence/1

https://synbiohub.org/public/igem/BBa_K199157_sequence/1
type: Sequence
persistent identity: BBa K199157 sequence
display id: BBa_K199157_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgccactgcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagatcaaaaaaatattctcaacataaaaaactttgtgtaatacttgtaacgtactagagggatccaattcggagagatgccggagcggctgaacggaccggtctagtggacaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactgcatatccttagcgaaagctaaggattttttttaagcttactagagatcaaaaaaatattctcaacataaaaaactttgtgtaatacttgtaacgtactagagggatccaattcggagagatgccggagcggctgaacggaccggtttgaccgacaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactgcatatccttagcgaaagctaaggattttttttaagct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0010_sequence/1
type: Sequence
persistent identity: BBa R0010 sequence
display id: BBa_R0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K199002_sequence/1
type: Sequence
persistent identity: BBa K199002 sequence
display id: BBa_K199002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggatccaattcggagagatgccggagcggctgaacggaccggtctagtggacaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactgcatatccttagcgaaagctaaggattttttttaagct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K199005_sequence/1
type: Sequence
persistent identity: BBa K199005 sequence
display id: BBa_K199005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgccactgcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K199028_sequence/1
type: Sequence
persistent identity: BBa K199028 sequence
display id: BBa_K199028_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggatccaattcggagagatgccggagcggctgaacggaccggtttgaccgacaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactgcatatccttagcgaaagctaaggattttttttaagct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K199106_sequence/1
type: Sequence
persistent identity: BBa K199106 sequence
display id: BBa_K199106_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atcaaaaaaatattctcaacataaaaaactttgtgtaatacttgtaacg
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z