LOCUS       BBa_K199160             1556 bp    DNA     linear       25-Nov-2024
DEFINITION  TT+pLux+RBS+LuxI(opti)+RBS+RFP
ACCESSION   BBa_K199160
VERSION     BBa_K199160.1  
FEATURES             Location/Qualifiers
     stem_loop       12..55
                     /label=stem_loop
     misc_feature    1..80
                     /label=BBa_B0010
     terminator      1..80
                     /label=BBa_B0010
     stem_loop       96..115
                     /label=T7 TE
     misc_feature    122..122
                     /label=stop
     polyA_site      116..129
                     /label=polya
     misc_feature    89..129
                     /label=BBa_B0012
     terminator      89..129
                     /label=BBa_B0012
     terminator      1..129
                     /label=BBa_B0015
     misc_feature    138..157
                     /label=LuxR/HSL
     promoter        157..162
                     /label=-35
     promoter        179..184
                     /label=-10
     misc_feature    190..190
                     /label=start
     misc_feature    138..192
                     /label=BBa_R0062
     promoter        138..192
                     /label=BBa_R0062
     misc_feature    205..208
                     /label=conserved
     RBS             201..212
                     /label=BBa_B0034
     CDS             219..824
                     /label=BBa_K199125
     misc_feature    837..840
                     /label=conserved
     RBS             833..844
                     /label=BBa_B0034
     CDS             851..1525
                     /label=mrfp1
     misc_feature    1532..1556
                     /label=Help:Barcodes
     CDS             851..1556
                     /label=BBa_E1010
ORIGIN
        1 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
       61 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
      121 gcgtttatat actagagacc tgtaggatcg tacaggttta cgcaagaaaa tggtttgtta
      181 tagtcgaata aatactagag aaagaggaga aatactagat ggcaattgca agcggtcgtg
      241 caaccattat gattaaaaaa agcgattttc tggccattcc gagcgaagaa tataaaggta
      301 ttctgagcct gcgctatcag gtttttaaac agcgtctgga atgggatctg gtggttgaaa
      361 ataatctgga aagtgatgaa tatgataata gcaatgccga atatatttat gcctgtgatg
      421 ataccgaaaa tgttagcggt tgttggcgtc tgctgccgac caccggtgat tatatgctga
      481 aaagcgtttt tccggaactg ctgggtcagc agagcgcacc gaaagatccg aatattgttg
      541 aactgagccg ttttgccgtg ggtaaaaata gcagcaaaat taataatagc gccagcgaaa
      601 ttaccatgaa actgtttgaa gccatttata aacatgccgt tagccagggt attaccgaat
      661 atgttaccgt taccagcacc gcaattgaac gttttctgaa acgtattaaa gtgccgtgtc
      721 atcgtattgg cgataaagaa attcatgttc tgggcgatac caaaagcgtt gttctgagca
      781 tgccgattaa tgaacagttt aaaaaagccg tgctgaacta ataatactag agaaagagga
      841 gaaatactag atggcttcct ccgaagacgt tatcaaagag ttcatgcgtt tcaaagttcg
      901 tatggaaggt tccgttaacg gtcacgagtt cgaaatcgaa ggtgaaggtg aaggtcgtcc
      961 gtacgaaggt acccagaccg ctaaactgaa agttaccaaa ggtggtccgc tgccgttcgc
     1021 ttgggacatc ctgtccccgc agttccagta cggttccaaa gcttacgtta aacacccggc
     1081 tgacatcccg gactacctga aactgtcctt cccggaaggt ttcaaatggg aacgtgttat
     1141 gaacttcgaa gacggtggtg ttgttaccgt tacccaggac tcctccctgc aagacggtga
     1201 gttcatctac aaagttaaac tgcgtggtac caacttcccg tccgacggtc cggttatgca
     1261 gaaaaaaacc atgggttggg aagcttccac cgaacgtatg tacccggaag acggtgctct
     1321 gaaaggtgaa atcaaaatgc gtctgaaact gaaagacggt ggtcactacg acgctgaagt
     1381 taaaaccacc tacatggcta aaaaaccggt tcagctgccg ggtgcttaca aaaccgacat
     1441 caaactggac atcacctccc acaacgaaga ctacaccatc gttgaacagt acgaacgtgc
     1501 tgaaggtcgt cactccaccg gtgcttaata acgctgatag tgctagtgta gatcgc
//