LOCUS       BBa_K199161             1573 bp    DNA     linear       22-Nov-2024
DEFINITION  TT+pLux+RBS+LuxI(opti)+RBS+GFP
ACCESSION   BBa_K199161
VERSION     BBa_K199161.1  
FEATURES             Location/Qualifiers
     stem_loop       12..55
                     /label=stem_loop
     misc_feature    1..80
                     /label=BBa_B0010
     terminator      1..80
                     /label=BBa_B0010
     stem_loop       96..115
                     /label=T7 TE
     misc_feature    122..122
                     /label=stop
     polyA_site      116..129
                     /label=polya
     misc_feature    89..129
                     /label=BBa_B0012
     terminator      89..129
                     /label=BBa_B0012
     misc_feature    138..157
                     /label=LuxR/HSL
     promoter        157..162
                     /label=-35
     promoter        179..184
                     /label=-10
     misc_feature    190..190
                     /label=start
     misc_feature    138..192
                     /label=BBa_R0062
     promoter        138..192
                     /label=BBa_R0062
     misc_feature    205..208
                     /label=conserved
     RBS             201..212
                     /label=BBa_B0034
     CDS             219..824
                     /label=BBa_K199125
     RBS             833..847
                     /label=RBS-1\Strong
     RBS             840..844
                     /label=RBS
     misc_feature    833..847
                     /label=BBa_B0030
     RBS             833..847
                     /label=BBa_B0030
     CDS             854..1573
                     /label=GFP protein
     CDS             854..1573
                     /label=BBa_E0040
ORIGIN
        1 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
       61 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
      121 gcgtttatat actagagacc tgtaggatcg tacaggttta cgcaagaaaa tggtttgtta
      181 tagtcgaata aatactagag aaagaggaga aatactagat ggcaattgca agcggtcgtg
      241 caaccattat gattaaaaaa agcgattttc tggccattcc gagcgaagaa tataaaggta
      301 ttctgagcct gcgctatcag gtttttaaac agcgtctgga atgggatctg gtggttgaaa
      361 ataatctgga aagtgatgaa tatgataata gcaatgccga atatatttat gcctgtgatg
      421 ataccgaaaa tgttagcggt tgttggcgtc tgctgccgac caccggtgat tatatgctga
      481 aaagcgtttt tccggaactg ctgggtcagc agagcgcacc gaaagatccg aatattgttg
      541 aactgagccg ttttgccgtg ggtaaaaata gcagcaaaat taataatagc gccagcgaaa
      601 ttaccatgaa actgtttgaa gccatttata aacatgccgt tagccagggt attaccgaat
      661 atgttaccgt taccagcacc gcaattgaac gttttctgaa acgtattaaa gtgccgtgtc
      721 atcgtattgg cgataaagaa attcatgttc tgggcgatac caaaagcgtt gttctgagca
      781 tgccgattaa tgaacagttt aaaaaagccg tgctgaacta ataatactag agattaaaga
      841 ggagaaatac tagatgcgta aaggagaaga acttttcact ggagttgtcc caattcttgt
      901 tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga
      961 tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc
     1021 atggccaaca cttgtcacta ctttcggtta tggtgttcaa tgctttgcga gatacccaga
     1081 tcatatgaaa cagcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaaag
     1141 aactatattt ttcaaagatg acgggaacta caagacacgt gctgaagtca agtttgaagg
     1201 tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag atggaaacat
     1261 tcttggacac aaattggaat acaactataa ctcacacaat gtatacatca tggcagacaa
     1321 acaaaagaat ggaatcaaag ttaacttcaa aattagacac aacattgaag atggaagcgt
     1381 tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc
     1441 agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg aaaagagaga
     1501 ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca tggatgaact
     1561 atacaaataa taa
//