LOCUS       BBa_K199162             2406 bp    DNA     linear       22-Dec-2024
DEFINITION  TT+pLux+RBS+LuxI(opti)+RBS+RFP+J23100+RBS+LuxR
ACCESSION   BBa_K199162
VERSION     BBa_K199162.1  
FEATURES             Location/Qualifiers
     stem_loop       12..55
                     /label=stem_loop
     misc_feature    1..80
                     /label=BBa_B0010
     terminator      1..80
                     /label=BBa_B0010
     stem_loop       96..115
                     /label=T7 TE
     misc_feature    122..122
                     /label=stop
     polyA_site      116..129
                     /label=polya
     misc_feature    89..129
                     /label=BBa_B0012
     terminator      89..129
                     /label=BBa_B0012
     terminator      1..129
                     /label=BBa_B0015
     misc_feature    138..157
                     /label=LuxR/HSL
     promoter        157..162
                     /label=-35
     promoter        179..184
                     /label=-10
     misc_feature    190..190
                     /label=start
     misc_feature    138..192
                     /label=BBa_R0062
     promoter        138..192
                     /label=BBa_R0062
     misc_feature    205..208
                     /label=conserved
     RBS             201..212
                     /label=BBa_B0034
     CDS             219..824
                     /label=BBa_K199125
     misc_feature    837..840
                     /label=conserved
     RBS             833..844
                     /label=BBa_B0034
     CDS             851..1525
                     /label=mrfp1
     misc_feature    1532..1556
                     /label=Help:Barcodes
     CDS             851..1556
                     /label=BBa_E1010
     misc_feature    1..1556
                     /label=BBa_K199160
     promoter        1565..1599
                     /label=BBa_J23100
     misc_feature    1612..1615
                     /label=conserved
     RBS             1608..1619
                     /label=BBa_B0034
     misc_feature    1626..1627
                     /label=prefix
     misc_feature    1799..1799
                     /label=T
     misc_feature    2117..2117
                     /label=A
     CDS             1626..2375
                     /label=luxR
     misc_feature    1626..2381
                     /label=BBa_C0062
     misc_feature    2382..2406
                     /label=Help:Barcodes
     CDS             1626..2381
                     /label=BBa_C0062
     misc_feature    1608..2406
                     /label=BBa_S01640
     misc_feature    1565..2406
                     /label=BBa_S03983
ORIGIN
        1 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
       61 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
      121 gcgtttatat actagagacc tgtaggatcg tacaggttta cgcaagaaaa tggtttgtta
      181 tagtcgaata aatactagag aaagaggaga aatactagat ggcaattgca agcggtcgtg
      241 caaccattat gattaaaaaa agcgattttc tggccattcc gagcgaagaa tataaaggta
      301 ttctgagcct gcgctatcag gtttttaaac agcgtctgga atgggatctg gtggttgaaa
      361 ataatctgga aagtgatgaa tatgataata gcaatgccga atatatttat gcctgtgatg
      421 ataccgaaaa tgttagcggt tgttggcgtc tgctgccgac caccggtgat tatatgctga
      481 aaagcgtttt tccggaactg ctgggtcagc agagcgcacc gaaagatccg aatattgttg
      541 aactgagccg ttttgccgtg ggtaaaaata gcagcaaaat taataatagc gccagcgaaa
      601 ttaccatgaa actgtttgaa gccatttata aacatgccgt tagccagggt attaccgaat
      661 atgttaccgt taccagcacc gcaattgaac gttttctgaa acgtattaaa gtgccgtgtc
      721 atcgtattgg cgataaagaa attcatgttc tgggcgatac caaaagcgtt gttctgagca
      781 tgccgattaa tgaacagttt aaaaaagccg tgctgaacta ataatactag agaaagagga
      841 gaaatactag atggcttcct ccgaagacgt tatcaaagag ttcatgcgtt tcaaagttcg
      901 tatggaaggt tccgttaacg gtcacgagtt cgaaatcgaa ggtgaaggtg aaggtcgtcc
      961 gtacgaaggt acccagaccg ctaaactgaa agttaccaaa ggtggtccgc tgccgttcgc
     1021 ttgggacatc ctgtccccgc agttccagta cggttccaaa gcttacgtta aacacccggc
     1081 tgacatcccg gactacctga aactgtcctt cccggaaggt ttcaaatggg aacgtgttat
     1141 gaacttcgaa gacggtggtg ttgttaccgt tacccaggac tcctccctgc aagacggtga
     1201 gttcatctac aaagttaaac tgcgtggtac caacttcccg tccgacggtc cggttatgca
     1261 gaaaaaaacc atgggttggg aagcttccac cgaacgtatg tacccggaag acggtgctct
     1321 gaaaggtgaa atcaaaatgc gtctgaaact gaaagacggt ggtcactacg acgctgaagt
     1381 taaaaccacc tacatggcta aaaaaccggt tcagctgccg ggtgcttaca aaaccgacat
     1441 caaactggac atcacctccc acaacgaaga ctacaccatc gttgaacagt acgaacgtgc
     1501 tgaaggtcgt cactccaccg gtgcttaata acgctgatag tgctagtgta gatcgctact
     1561 agagttgacg gctagctcag tcctaggtac agtgctagct actagagaaa gaggagaaat
     1621 actagatgaa aaacataaat gccgacgaca catacagaat aattaataaa attaaagctt
     1681 gtagaagcaa taatgatatt aatcaatgct tatctgatat gactaaaatg gtacattgtg
     1741 aatattattt actcgcgatc atttatcctc attctatggt taaatctgat atttcaatcc
     1801 tagataatta ccctaaaaaa tggaggcaat attatgatga cgctaattta ataaaatatg
     1861 atcctatagt agattattct aactccaatc attcaccaat taattggaat atatttgaaa
     1921 acaatgctgt aaataaaaaa tctccaaatg taattaaaga agcgaaaaca tcaggtctta
     1981 tcactgggtt tagtttccct attcatacgg ctaacaatgg cttcggaatg cttagttttg
     2041 cacattcaga aaaagacaac tatatagata gtttattttt acatgcgtgt atgaacatac
     2101 cattaattgt tccttctcta gttgataatt atcgaaaaat aaatatagca aataataaat
     2161 caaacaacga tttaaccaaa agagaaaaag aatgtttagc gtgggcatgc gaaggaaaaa
     2221 gctcttggga tatttcaaaa atattaggtt gcagtgagcg tactgtcact ttccatttaa
     2281 ccaatgcgca aatgaaactc aatacaacaa accgctgcca aagtatttct aaagcaattt
     2341 taacaggagc aattgattgc ccatacttta aaaattaata acactgatag tgctagtgta
     2401 gatcac
//