##gff-version 3
##sequence-region BBa_K199162 1 2406
BBa_K199162	.	engineered_region	1	1556	.	+	0	ID=BBa_K199160;Name=BBa_K199160
BBa_K199162	.	ribosome_entry_site	201	212	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K199160
BBa_K199162	.	conserved_region	205	208	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K199162	.	CDS	219	824	.	+	0	ID=BBa_K199125;Name=BBa_K199125;Parent=BBa_K199160
BBa_K199162	.	CDS	851	1556	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_K199160
BBa_K199162	.	CDS	851	1525	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K199162	.	engineered_tag	1532	1556	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K199162	.	ribosome_entry_site	833	844	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K199160
BBa_K199162	.	conserved_region	837	840	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K199162	.	terminator	1	129	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K199160
BBa_K199162	.	terminator	1	80	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K199162	.	stem_loop	12	55	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K199162	.	engineered_region	1	80	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K199162	.	terminator	89	129	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K199162	.	engineered_region	89	129	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K199162	.	stop_codon	122	122	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K199162	.	polyA_site	116	129	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K199162	.	stem_loop	96	115	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K199162	.	promoter	138	192	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_K199160
BBa_K199162	.	promoter	157	162	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K199162	.	engineered_region	138	192	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K199162	.	start_codon	190	190	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K199162	.	promoter	179	184	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K199162	.	non_covalent_binding_site	138	157	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K199162	.	engineered_region	1565	2406	.	+	0	ID=BBa_S03983;Name=BBa_S03983
BBa_K199162	.	engineered_region	1608	2406	.	+	0	ID=BBa_S01640;Name=BBa_S01640;Parent=BBa_S03983
BBa_K199162	.	CDS	1626	2381	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_S01640
BBa_K199162	.	point_mutation	2117	2117	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K199162	.	engineered_region	1626	2381	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K199162	.	engineered_region	1626	1627	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K199162	.	CDS	1626	2375	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K199162	.	point_mutation	1799	1799	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K199162	.	engineered_tag	2382	2406	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K199162	.	ribosome_entry_site	1608	1619	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S01640
BBa_K199162	.	conserved_region	1612	1615	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K199162	.	promoter	1565	1599	.	+	0	ID=BBa_J23100;Name=BBa_J23100;Parent=BBa_S03983
>BBa_K199162
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtt
tacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatactagatggcaattgcaagcggtc
gtgcaaccattatgattaaaaaaagcgattttctggccattccgagcgaagaatataaaggtattctgagcctgcgcta
tcaggtttttaaacagcgtctggaatgggatctggtggttgaaaataatctggaaagtgatgaatatgataatagcaat
gccgaatatatttatgcctgtgatgataccgaaaatgttagcggttgttggcgtctgctgccgaccaccggtgattata
tgctgaaaagcgtttttccggaactgctgggtcagcagagcgcaccgaaagatccgaatattgttgaactgagccgttt
tgccgtgggtaaaaatagcagcaaaattaataatagcgccagcgaaattaccatgaaactgtttgaagccatttataaa
catgccgttagccagggtattaccgaatatgttaccgttaccagcaccgcaattgaacgttttctgaaacgtattaaag
tgccgtgtcatcgtattggcgataaagaaattcatgttctgggcgataccaaaagcgttgttctgagcatgccgattaa
tgaacagtttaaaaaagccgtgctgaactaataatactagagaaagaggagaaatactagatggcttcctccgaagacg
ttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaagg
tgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggac
atcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgt
ccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctc
cctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaa
aaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtc
tgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccggg
tgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgct
gaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagttgacggctagctcag
tcctaggtacagtgctagctactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaa
taattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattg
tgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaa
aaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcac
caattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcagg
tcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaa
gacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatc
gaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcga
aggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaa
atgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatacttta
aaaattaataacactgatagtgctagtgtagatcac