LOCUS       BBa_K199163             1577 bp    DNA     linear       22-Nov-2024
DEFINITION  TT+pLux+RBS+LuxI(2 sites)+RBS+RFP
ACCESSION   BBa_K199163
VERSION     BBa_K199163.1  
FEATURES             Location/Qualifiers
     stem_loop       12..55
                     /label=stem_loop
     misc_feature    1..80
                     /label=BBa_B0010
     terminator      1..80
                     /label=BBa_B0010
     stem_loop       96..115
                     /label=T7 TE
     misc_feature    122..122
                     /label=stop
     polyA_site      116..129
                     /label=polya
     misc_feature    89..129
                     /label=BBa_B0012
     terminator      89..129
                     /label=BBa_B0012
     misc_feature    138..157
                     /label=LuxR/HSL
     promoter        157..162
                     /label=-35
     promoter        179..184
                     /label=-10
     misc_feature    190..190
                     /label=start
     misc_feature    138..192
                     /label=BBa_R0062
     promoter        138..192
                     /label=BBa_R0062
     misc_feature    205..208
                     /label=conserved
     RBS             201..212
                     /label=BBa_B0034
     misc_feature    222..229
                     /label=AsiSI Site
     misc_feature    235..242
                     /label=AscI Site
     CDS             219..845
                     /label=BBa_K199130
     misc_feature    1..845
                     /label=BBa_S04424
     misc_feature    858..861
                     /label=conserved
     RBS             854..865
                     /label=BBa_B0034
     CDS             872..1546
                     /label=mrfp1
     misc_feature    1553..1577
                     /label=Help:Barcodes
     CDS             872..1577
                     /label=BBa_E1010
     misc_feature    854..1577
                     /label=BBa_S04419
ORIGIN
        1 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
       61 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
      121 gcgtttatat actagagacc tgtaggatcg tacaggttta cgcaagaaaa tggtttgtta
      181 tagtcgaata aatactagag aaagaggaga aatactagat ggcgatcgca tctgggcgcg
      241 ccgcaattgc aagcggtcgt gcaaccatta tgattaaaaa aagcgatttt ctggccattc
      301 cgagcgaaga atataaaggt attctgagcc tgcgctatca ggtttttaaa cagcgtctgg
      361 aatgggatct ggtggttgaa aataatctgg aaagtgatga atatgataat agcaatgccg
      421 aatatattta tgcctgtgat gataccgaaa atgttagcgg ttgttggcgt ctgctgccga
      481 ccaccggtga ttatatgctg aaaagcgttt ttccggaact gctgggtcag cagagcgcac
      541 cgaaagatcc gaatattgtt gaactgagcc gttttgccgt gggtaaaaat agcagcaaaa
      601 ttaataatag cgccagcgaa attaccatga aactgtttga agccatttat aaacatgccg
      661 ttagccaggg tattaccgaa tatgttaccg ttaccagcac cgcaattgaa cgttttctga
      721 aacgtattaa agtgccgtgt catcgtattg gcgataaaga aattcatgtt ctgggcgata
      781 ccaaaagcgt tgttctgagc atgccgatta atgaacagtt taaaaaagcc gtgctgaact
      841 aataatacta gagaaagagg agaaatacta gatggcttcc tccgaagacg ttatcaaaga
      901 gttcatgcgt ttcaaagttc gtatggaagg ttccgttaac ggtcacgagt tcgaaatcga
      961 aggtgaaggt gaaggtcgtc cgtacgaagg tacccagacc gctaaactga aagttaccaa
     1021 aggtggtccg ctgccgttcg cttgggacat cctgtccccg cagttccagt acggttccaa
     1081 agcttacgtt aaacacccgg ctgacatccc ggactacctg aaactgtcct tcccggaagg
     1141 tttcaaatgg gaacgtgtta tgaacttcga agacggtggt gttgttaccg ttacccagga
     1201 ctcctccctg caagacggtg agttcatcta caaagttaaa ctgcgtggta ccaacttccc
     1261 gtccgacggt ccggttatgc agaaaaaaac catgggttgg gaagcttcca ccgaacgtat
     1321 gtacccggaa gacggtgctc tgaaaggtga aatcaaaatg cgtctgaaac tgaaagacgg
     1381 tggtcactac gacgctgaag ttaaaaccac ctacatggct aaaaaaccgg ttcagctgcc
     1441 gggtgcttac aaaaccgaca tcaaactgga catcacctcc cacaacgaag actacaccat
     1501 cgttgaacag tacgaacgtg ctgaaggtcg tcactccacc ggtgcttaat aacgctgata
     1561 gtgctagtgt agatcgc
//