LOCUS       BBa_K199165              907 bp    DNA     linear       23-Dec-2024
DEFINITION  pT7 + RBS + GFP + TT in pSB3T5
ACCESSION   BBa_K199165
VERSION     BBa_K199165.1  
FEATURES             Location/Qualifiers
     misc_feature    18..18
                     /label=Transcription Start Site
     misc_feature    1..11
                     /label=Polymerase Binding Region
     misc_feature    12..23
                     /label=Initiation Region
     promoter        1..23
                     /label=BBa_R0085
     RBS             32..44
                     /label=RBS-3\Weak
     RBS             38..41
                     /label=RBS
     misc_feature    32..44
                     /label=BBa_B0032
     RBS             32..44
                     /label=BBa_B0032
     CDS             51..770
                     /label=GFP protein
     CDS             51..770
                     /label=BBa_E0040
     stem_loop       790..833
                     /label=stem_loop
     misc_feature    779..858
                     /label=BBa_B0010
     terminator      779..858
                     /label=BBa_B0010
     stem_loop       874..893
                     /label=T7 TE
     misc_feature    900..900
                     /label=stop
     polyA_site      894..907
                     /label=polya
     misc_feature    867..907
                     /label=BBa_B0012
     terminator      867..907
                     /label=BBa_B0012
ORIGIN
        1 taatacgact cactataggg agatactaga gtcacacagg aaagtactag atgcgtaaag
       61 gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt gatgttaatg
      121 ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga aaacttaccc
      181 ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt gtcactactt
      241 tcggttatgg tgttcaatgc tttgcgagat acccagatca tatgaaacag catgactttt
      301 tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc aaagatgacg
      361 ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg
      421 agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa ttggaataca
      481 actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga atcaaagtta
      541 acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac cattatcaac
      601 aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac ctgtccacac
      661 aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt cttgagtttg
      721 taacagctgc tgggattaca catggcatgg atgaactata caaataataa tactagagcc
      781 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
      841 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc
      901 gtttata
//