##gff-version 3
##sequence-region BBa_K199167 1 2522
BBa_K199167	.	engineered_region	1586	2522	.	+	0	ID=BBa_K091166;Name=BBa_K091166
BBa_K199167	.	engineered_region	1724	2522	.	+	0	ID=BBa_J37033;Name=BBa_J37033;Parent=BBa_K091166
BBa_K199167	.	ribosome_entry_site	1724	1735	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J37033
BBa_K199167	.	conserved_region	1728	1731	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K199167	.	CDS	1742	2497	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_J37033
BBa_K199167	.	CDS	1742	2491	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K199167	.	engineered_region	1742	1743	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K199167	.	engineered_tag	2498	2522	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K199167	.	point_mutation	2233	2233	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K199167	.	engineered_region	1742	2497	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K199167	.	point_mutation	1915	1915	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K199167	.	promoter	1586	1715	.	+	0	ID=BBa_I13453;Name=BBa_I13453;Parent=BBa_K091166
BBa_K199167	.	engineered_region	1	1577	.	+	0	ID=BBa_K199163;Name=BBa_K199163
BBa_K199167	.	engineered_region	1	845	.	+	0	ID=BBa_S04424;Name=BBa_S04424;Parent=BBa_K199163
BBa_K199167	.	terminator	89	129	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_S04424
BBa_K199167	.	stem_loop	96	115	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K199167	.	engineered_region	89	129	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K199167	.	stop_codon	122	122	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K199167	.	polyA_site	116	129	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K199167	.	CDS	219	845	.	+	0	ID=BBa_K199130;Name=BBa_K199130;Parent=BBa_S04424
BBa_K199167	.	sequence_feature	235	242	.	+	0	ID=annotation2120505;Name=AscI Site;Parent=BBa_K199130
BBa_K199167	.	sequence_feature	222	229	.	+	0	ID=annotation2120504;Name=AsiSI Site;Parent=BBa_K199130
BBa_K199167	.	terminator	1	80	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_S04424
BBa_K199167	.	stem_loop	12	55	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K199167	.	engineered_region	1	80	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K199167	.	promoter	138	192	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_S04424
BBa_K199167	.	non_covalent_binding_site	138	157	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K199167	.	engineered_region	138	192	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K199167	.	start_codon	190	190	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K199167	.	promoter	179	184	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K199167	.	promoter	157	162	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K199167	.	ribosome_entry_site	201	212	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S04424
BBa_K199167	.	conserved_region	205	208	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K199167	.	engineered_region	854	1577	.	+	0	ID=BBa_S04419;Name=BBa_S04419;Parent=BBa_K199163
BBa_K199167	.	ribosome_entry_site	854	865	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S04419
BBa_K199167	.	conserved_region	858	861	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K199167	.	CDS	872	1577	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_S04419
BBa_K199167	.	CDS	872	1546	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K199167	.	engineered_tag	1553	1577	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
>BBa_K199167
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtt
tacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatactagatggcgatcgcatctgggc
gcgccgcaattgcaagcggtcgtgcaaccattatgattaaaaaaagcgattttctggccattccgagcgaagaatataa
aggtattctgagcctgcgctatcaggtttttaaacagcgtctggaatgggatctggtggttgaaaataatctggaaagt
gatgaatatgataatagcaatgccgaatatatttatgcctgtgatgataccgaaaatgttagcggttgttggcgtctgc
tgccgaccaccggtgattatatgctgaaaagcgtttttccggaactgctgggtcagcagagcgcaccgaaagatccgaa
tattgttgaactgagccgttttgccgtgggtaaaaatagcagcaaaattaataatagcgccagcgaaattaccatgaaa
ctgtttgaagccatttataaacatgccgttagccagggtattaccgaatatgttaccgttaccagcaccgcaattgaac
gttttctgaaacgtattaaagtgccgtgtcatcgtattggcgataaagaaattcatgttctgggcgataccaaaagcgt
tgttctgagcatgccgattaatgaacagtttaaaaaagccgtgctgaactaataatactagagaaagaggagaaatact
agatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacga
gttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggt
ccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgaca
tcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgt
taccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtcc
gacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctga
aaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaa
aaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatc
gttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctac
tagagacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacc
tgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatac
tagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatatta
atcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggt
taaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatat
gatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaa
aatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaa
tggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaac
ataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaa
ccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcag
tgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaa
gcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcac