BBa_K199167
type: ComponentDefinition
persistent identity: BBa K199167
display id: BBa_K199167
version: 1
was generated by: iGEM to SBOL conversion
description: TT+pLux+RBS+LuxI(2 sites)+RBS+RFP+pBad+RBS+LuxR
created: 2010-06-16T11:00:00Z
modified: 2015-05-08T01:11:20Z
mutable provenance: BioBricks
owned by: james
owned by: myers
mutable description: TT+pLux+RBS+LuxI(2 sites)+RBS+RFP+pBad+RBS+LuxR
discontinued: false
dominant: false
group u list: _295_
m user id: 0
owner id: 5111
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: N/A
star: false
creator: Eric Sawyer
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_K199163
component: BBa_K091166
sequence annotation: BBa_K199163
sequence annotation: BBa_K091166
sequence: https://synbiohub.org/public/igem/BBa_K199167_sequence/1

BBa_K199163
type: Component
persistent identity: component2288571
display id: component2288571
version: 1
top level: BBa_K199167
definition: BBa_K199163
access: public

BBa_K091166
type: Component
persistent identity: component2288582
display id: component2288582
version: 1
top level: BBa_K199167
definition: BBa_K091166
access: public

BBa_K199163
type: SequenceAnnotation
persistent identity: annotation2288571
display id: annotation2288571
version: 1
top level: BBa_K199167
location: https://synbiohub.org/public/igem/BBa_K199167/annotation2288571/range2288571/1
component: BBa_K199163

https://synbiohub.org/public/igem/BBa_K199167/annotation2288571/range2288571/1
type: Range
persistent identity: range2288571
display id: range2288571
version: 1
top level: BBa_K199167
direction: direction/reverse
start: 1
end: 1577
orientation: inline

BBa_K091166
type: SequenceAnnotation
persistent identity: annotation2288582
display id: annotation2288582
version: 1
top level: BBa_K199167
location: https://synbiohub.org/public/igem/BBa_K199167/annotation2288582/range2288582/1
component: BBa_K091166

https://synbiohub.org/public/igem/BBa_K199167/annotation2288582/range2288582/1
type: Range
persistent identity: range2288582
display id: range2288582
version: 1
top level: BBa_K199167
direction: direction/reverse
start: 1586
end: 2522
orientation: inline

BBa_K091166
type: ComponentDefinition
persistent identity: BBa K091166
display id: BBa_K091166
version: 1
was generated by: iGEM to SBOL conversion
description: pBAD-RBS-luxR
created: 2008-07-30T11:00:00Z
modified: 2015-05-08T01:08:38Z
mutable provenance: Registry
owned by: james
owned by: myers
mutable description: This is a composite part of pBAD, B0034, and LuxR. LuxR are is the receiver protein for the quorum sensing molecule AI-1 produce by LuxI.
discontinued: false
dominant: false
experience: experience/Works
group u list: _191_
m user id: 0
owner id: 3129
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: none
star: false
creator: Andrew Gordon
type: DnaRegion
role: SO:0000804
role: partType/Signalling
component: BBa_I13453
component: BBa_J37033
sequence annotation: BBa_I13453
sequence annotation: BBa_J37033
sequence: https://synbiohub.org/public/igem/BBa_K091166_sequence/1

BBa_I13453
type: Component
persistent identity: component2245935
display id: component2245935
version: 1
top level: BBa_K091166
definition: BBa_I13453
access: public

BBa_J37033
type: Component
persistent identity: component2245944
display id: component2245944
version: 1
top level: BBa_K091166
definition: BBa_J37033
access: public

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation2245935
display id: annotation2245935
version: 1
top level: BBa_K091166
location: https://synbiohub.org/public/igem/BBa_K091166/annotation2245935/range2245935/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_K091166/annotation2245935/range2245935/1
type: Range
persistent identity: range2245935
display id: range2245935
version: 1
top level: BBa_K091166
direction: direction/reverse
start: 1
end: 130
orientation: inline

BBa_J37033
type: SequenceAnnotation
persistent identity: annotation2245944
display id: annotation2245944
version: 1
top level: BBa_K091166
location: https://synbiohub.org/public/igem/BBa_K091166/annotation2245944/range2245944/1
component: BBa_J37033

https://synbiohub.org/public/igem/BBa_K091166/annotation2245944/range2245944/1
type: Range
persistent identity: range2245944
display id: range2245944
version: 1
top level: BBa_K091166
direction: direction/reverse
start: 139
end: 937
orientation: inline

BBa_K199163
type: ComponentDefinition
persistent identity: BBa K199163
display id: BBa_K199163
version: 1
was generated by: iGEM to SBOL conversion
description: TT+pLux+RBS+LuxI(2 sites)+RBS+RFP
created: 2010-06-08T11:00:00Z
modified: 2015-05-08T01:11:20Z
mutable provenance: BioBricks
owned by: james
owned by: myers
mutable description: TT+pLux+RBS+LuxI(2 sites)+RBS+RFP
discontinued: false
dominant: false
group u list: _295_
m user id: 0
owner id: 5111
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: N/A
star: false
creator: Eric Sawyer
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_S04424
component: BBa_S04419
sequence annotation: BBa_S04424
sequence annotation: BBa_S04419
sequence: https://synbiohub.org/public/igem/BBa_K199163_sequence/1

BBa_S04424
type: Component
persistent identity: component2268292
display id: component2268292
version: 1
top level: BBa_K199163
definition: BBa_S04424
access: public

BBa_S04419
type: Component
persistent identity: component2268298
display id: component2268298
version: 1
top level: BBa_K199163
definition: BBa_S04419
access: public

BBa_S04424
type: SequenceAnnotation
persistent identity: annotation2268292
display id: annotation2268292
version: 1
top level: BBa_K199163
location: https://synbiohub.org/public/igem/BBa_K199163/annotation2268292/range2268292/1
component: BBa_S04424

https://synbiohub.org/public/igem/BBa_K199163/annotation2268292/range2268292/1
type: Range
persistent identity: range2268292
display id: range2268292
version: 1
top level: BBa_K199163
direction: direction/reverse
start: 1
end: 845
orientation: inline

BBa_S04419
type: SequenceAnnotation
persistent identity: annotation2268298
display id: annotation2268298
version: 1
top level: BBa_K199163
location: https://synbiohub.org/public/igem/BBa_K199163/annotation2268298/range2268298/1
component: BBa_S04419

https://synbiohub.org/public/igem/BBa_K199163/annotation2268298/range2268298/1
type: Range
persistent identity: range2268298
display id: range2268298
version: 1
top level: BBa_K199163
direction: direction/reverse
start: 854
end: 1577
orientation: inline

BBa_I13453
type: ComponentDefinition
persistent identity: BBa I13453
display id: BBa_I13453
version: 1
was generated by: iGEM to SBOL conversion
description: Pbad promoter
created: 2005-05-24T11:00:00Z
modified: 2015-08-31T04:07:34Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: PBad promoter from I0500 without AraC.
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jkm
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I13453_sequence/1

BBa_J37033
type: ComponentDefinition
persistent identity: BBa J37033
display id: BBa_J37033
version: 1
was generated by: iGEM to SBOL conversion
description: RBS + LuxR
created: 2006-08-22T11:00:00Z
modified: 2015-08-31T04:08:48Z
mutable provenance: Ligation of B0034 + C0062
owned by: james
owned by: myers
mutable description: LuxR coding region with RBS
discontinued: false
dominant: false
experience: experience/None
group u list: _66_
m user id: 0
owner id: 1052
owning group id: 66
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: Designed for Imperial College iGEM 2006
star: true
creator: John Sy
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_B0034
component: BBa_C0062
sequence annotation: BBa_B0034
sequence annotation: BBa_C0062
sequence: https://synbiohub.org/public/igem/BBa_J37033_sequence/1

BBa_B0034
type: Component
persistent identity: component2243716
display id: component2243716
version: 1
top level: BBa_J37033
definition: BBa_B0034
access: public

BBa_C0062
type: Component
persistent identity: component2243719
display id: component2243719
version: 1
top level: BBa_J37033
definition: luxr
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2243716
display id: annotation2243716
version: 1
top level: BBa_J37033
location: https://synbiohub.org/public/igem/BBa_J37033/annotation2243716/range2243716/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J37033/annotation2243716/range2243716/1
type: Range
persistent identity: range2243716
display id: range2243716
version: 1
top level: BBa_J37033
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation2243719
display id: annotation2243719
version: 1
top level: BBa_J37033
location: https://synbiohub.org/public/igem/BBa_J37033/annotation2243719/range2243719/1
component: BBa_C0062

https://synbiohub.org/public/igem/BBa_J37033/annotation2243719/range2243719/1
type: Range
persistent identity: range2243719
display id: range2243719
version: 1
top level: BBa_J37033
direction: direction/forward
start: 19
end: 774
orientation: inline

BBa_S04419
type: ComponentDefinition
persistent identity: BBa S04419
display id: BBa_S04419
version: 1
was generated by: iGEM to SBOL conversion
description: B0034:E1010
created: 2010-01-22T12:00:00Z
modified: 2015-05-08T01:14:39Z
owned by: james
owned by: myers
part status: Released HQ 2013
discontinued: false
dominant: false
group u list: _9_
m user id: 0
owner id: 5562
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
star: false
creator: Mary Gearing
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_E1010
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_E1010
sequence: https://synbiohub.org/public/igem/BBa_S04419_sequence/1

BBa_E1010
type: Component
persistent identity: component2244399
display id: component2244399
version: 1
top level: BBa_S04419
definition: mRFP1
access: public

BBa_B0034
type: Component
persistent identity: component2244396
display id: component2244396
version: 1
top level: BBa_S04419
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2244396
display id: annotation2244396
version: 1
top level: BBa_S04419
location: https://synbiohub.org/public/igem/BBa_S04419/annotation2244396/range2244396/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S04419/annotation2244396/range2244396/1
type: Range
persistent identity: range2244396
display id: range2244396
version: 1
top level: BBa_S04419
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2244399
display id: annotation2244399
version: 1
top level: BBa_S04419
location: https://synbiohub.org/public/igem/BBa_S04419/annotation2244399/range2244399/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_S04419/annotation2244399/range2244399/1
type: Range
persistent identity: range2244399
display id: range2244399
version: 1
top level: BBa_S04419
direction: direction/reverse
start: 19
end: 724
orientation: inline

BBa_S04424
type: ComponentDefinition
persistent identity: BBa S04424
display id: BBa_S04424
version: 1
was generated by: iGEM to SBOL conversion
description: K199114:K199130
created: 2010-03-28T11:00:00Z
modified: 2015-05-08T01:14:40Z
owned by: james
owned by: myers
discontinued: false
dominant: false
group u list: _9_
m user id: 0
owner id: 5562
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
star: false
creator: Mary Gearing
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_K199130
component: BBa_B0012
component: BBa_B0010
component: BBa_B0034
component: BBa_R0062
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_R0062
sequence annotation: BBa_B0034
sequence annotation: BBa_K199130
sequence: https://synbiohub.org/public/igem/BBa_S04424_sequence/1

BBa_K199130
type: Component
persistent identity: component2067430
display id: component2067430
version: 1
top level: BBa_S04424
definition: BBa_K199130
access: public

BBa_B0012
type: Component
persistent identity: component2067419
display id: component2067419
version: 1
top level: BBa_S04424
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component2067417
display id: component2067417
version: 1
top level: BBa_S04424
definition: BBa_B0010
access: public

BBa_B0034
type: Component
persistent identity: component2067429
display id: component2067429
version: 1
top level: BBa_S04424
definition: BBa_B0034
access: public

BBa_R0062
type: Component
persistent identity: component2067424
display id: component2067424
version: 1
top level: BBa_S04424
definition: lux pR
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2067417
display id: annotation2067417
version: 1
top level: BBa_S04424
location: https://synbiohub.org/public/igem/BBa_S04424/annotation2067417/range2067417/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_S04424/annotation2067417/range2067417/1
type: Range
persistent identity: range2067417
display id: range2067417
version: 1
top level: BBa_S04424
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2067419
display id: annotation2067419
version: 1
top level: BBa_S04424
location: https://synbiohub.org/public/igem/BBa_S04424/annotation2067419/range2067419/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_S04424/annotation2067419/range2067419/1
type: Range
persistent identity: range2067419
display id: range2067419
version: 1
top level: BBa_S04424
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2067424
display id: annotation2067424
version: 1
top level: BBa_S04424
location: https://synbiohub.org/public/igem/BBa_S04424/annotation2067424/range2067424/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_S04424/annotation2067424/range2067424/1
type: Range
persistent identity: range2067424
display id: range2067424
version: 1
top level: BBa_S04424
direction: direction/forward
start: 138
end: 192
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2067429
display id: annotation2067429
version: 1
top level: BBa_S04424
location: https://synbiohub.org/public/igem/BBa_S04424/annotation2067429/range2067429/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_S04424/annotation2067429/range2067429/1
type: Range
persistent identity: range2067429
display id: range2067429
version: 1
top level: BBa_S04424
direction: direction/reverse
start: 201
end: 212
orientation: inline

BBa_K199130
type: SequenceAnnotation
persistent identity: annotation2067430
display id: annotation2067430
version: 1
top level: BBa_S04424
location: https://synbiohub.org/public/igem/BBa_S04424/annotation2067430/range2067430/1
component: BBa_K199130

https://synbiohub.org/public/igem/BBa_S04424/annotation2067430/range2067430/1
type: Range
persistent identity: range2067430
display id: range2067430
version: 1
top level: BBa_S04424
direction: direction/reverse
start: 219
end: 845
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

mRFP1
type: ComponentDefinition
persistent identity: BBa E1010
display id: BBa_E1010
version: 1
was generated by: iGEM to SBOL conversion
description: **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
created: 2004-07-27T11:00:00Z
modified: 2015-08-31T04:07:26Z
mutable provenance: Campbell et al., PNAS v99 p7877 URL
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 52
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
star: true
creator: Drew Endy
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: mrfp1
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_E1010_sequence/1

mrfp1
type: SequenceAnnotation
persistent identity: annotation1014044
display id: annotation1014044
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
type: Range
persistent identity: range1014044
display id: range1014044
version: 1
top level: mRFP1
direction: direction/forward
start: 1
end: 675
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214014
display id: annotation2214014
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
type: Range
persistent identity: range2214014
display id: range2214014
version: 1
top level: mRFP1
direction: direction/forward
start: 682
end: 706
orientation: inline

luxr
type: ComponentDefinition
persistent identity: BBa C0062
display id: BBa_C0062
version: 1
was generated by: iGEM to SBOL conversion
description: luxR repressor/activator, (no LVA?)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: V. fischeri <genbank>AF170104</genbank>
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: In complex with HSL, LuxR binds to the Lux promoter, activating transcription from Pr <bb_part>BBa_R0062</bb_part>, and repressing transcription from Pl <bb_part>BBa_R0063</bb_part>. The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux activator, LuxR complexed to HSL. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: 2 silent point mutants were introduced in the coding sequence to remove internal XbaI and PstI sites. Mutation sites were chosen to replace codons commonly used in E. coli with codons used at a similar frequency.
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: luxR
sequence annotation: prefix
sequence annotation: BBa_C0062
sequence annotation: T
sequence annotation: A
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0062_sequence/1

luxR
type: SequenceAnnotation
persistent identity: annotation1766
display id: annotation1766
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1766/range1766/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0062/annotation1766/range1766/1
type: Range
persistent identity: range1766
display id: range1766
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 750
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation1762
display id: annotation1762
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1762/range1762/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_C0062/annotation1762/range1762/1
type: Range
persistent identity: range1762
display id: range1762
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 2
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation7039
display id: annotation7039
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation7039/range7039/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_C0062/annotation7039/range7039/1
type: Range
persistent identity: range7039
display id: range7039
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 756
orientation: inline

T
type: SequenceAnnotation
persistent identity: annotation1764
display id: annotation1764
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1764/range1764/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_C0062/annotation1764/range1764/1
type: Range
persistent identity: range1764
display id: range1764
version: 1
top level: luxr
direction: direction/forward
start: 174
end: 174
orientation: inline

A
type: SequenceAnnotation
persistent identity: annotation1765
display id: annotation1765
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1765/range1765/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_C0062/annotation1765/range1765/1
type: Range
persistent identity: range1765
display id: range1765
version: 1
top level: luxr
direction: direction/forward
start: 492
end: 492
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213986
display id: annotation2213986
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation2213986/range2213986/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0062/annotation2213986/range2213986/1
type: Range
persistent identity: range2213986
display id: range2213986
version: 1
top level: luxr
direction: direction/forward
start: 757
end: 781
orientation: inline

lux pR
type: ComponentDefinition
persistent identity: BBa R0062
display id: BBa_R0062
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (luxR & HSL regulated -- lux pR)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: V. fischeri
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Promoter activated by LuxR in concert with HSL The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the LuxR activator protein complexed with the autoinducer, 3-oxo-hexanoyl-HSL. Two molecules of LuxR protein form a complex with two molecules of the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: This promoter is based on the Vibrio fischeri quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts <bb_part>BBa_R0062</bb_part> and <bb_part>BBa_R0063</bb_part> were derived is shown in the picture below. <img src="<bb_file>Image1.gif</bb_file>" width="614" height="362">
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: BBa_R0062
sequence annotation: LuxR/HSL
sequence annotation: -35
sequence annotation: -10
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0062_sequence/1

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation7070
display id: annotation7070
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation7070/range7070/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0062/annotation7070/range7070/1
type: Range
persistent identity: range7070
display id: range7070
version: 1
top level: lux pR
direction: direction/forward
start: 1
end: 55
orientation: inline

LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2045
display id: annotation2045
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2045/range2045/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0062/annotation2045/range2045/1
type: Range
persistent identity: range2045
display id: range2045
version: 1
top level: lux pR
direction: direction/forward
start: 1
end: 20
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2046
display id: annotation2046
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2046/range2046/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0062/annotation2046/range2046/1
type: Range
persistent identity: range2046
display id: range2046
version: 1
top level: lux pR
direction: direction/forward
start: 20
end: 25
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2047
display id: annotation2047
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2047/range2047/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0062/annotation2047/range2047/1
type: Range
persistent identity: range2047
display id: range2047
version: 1
top level: lux pR
direction: direction/forward
start: 42
end: 47
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2048
display id: annotation2048
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2048/range2048/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0062/annotation2048/range2048/1
type: Range
persistent identity: range2048
display id: range2048
version: 1
top level: lux pR
direction: direction/forward
start: 53
end: 53
orientation: inline

BBa_K199130
type: ComponentDefinition
persistent identity: BBa K199130
display id: BBa_K199130
version: 1
was generated by: iGEM to SBOL conversion
description: LuxI with 2 sites
created: 2010-03-28T11:00:00Z
modified: 2015-05-08T01:11:20Z
mutable provenance: genomic LuxI + 42 bp, this includes AscI and AsiSI sites
owned by: james
owned by: myers
mutable description: This is LuxI optimized for E. coli. It contains 2 restriction sites (AscI and AsiSI). This facilitates the insertion of different frameshift mutations.
discontinued: false
dominant: false
group u list: _295_
m user id: 0
owner id: 5562
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: n/a
star: false
creator: Mary Gearing
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: AsiSI Site
sequence annotation: AscI Site
sequence: https://synbiohub.org/public/igem/BBa_K199130_sequence/1

AsiSI Site
type: SequenceAnnotation
persistent identity: annotation2120504
display id: annotation2120504
version: 1
top level: BBa_K199130
location: https://synbiohub.org/public/igem/BBa_K199130/annotation2120504/range2120504/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K199130/annotation2120504/range2120504/1
type: Range
persistent identity: range2120504
display id: range2120504
version: 1
top level: BBa_K199130
direction: direction/forward
start: 4
end: 11
orientation: inline

AscI Site
type: SequenceAnnotation
persistent identity: annotation2120505
display id: annotation2120505
version: 1
top level: BBa_K199130
location: https://synbiohub.org/public/igem/BBa_K199130/annotation2120505/range2120505/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K199130/annotation2120505/range2120505/1
type: Range
persistent identity: range2120505
display id: range2120505
version: 1
top level: BBa_K199130
direction: direction/forward
start: 17
end: 24
orientation: inline

https://synbiohub.org/public/igem/BBa_K199167_sequence/1
type: Sequence
persistent identity: BBa K199167 sequence
display id: BBa_K199167_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatactagatggcgatcgcatctgggcgcgccgcaattgcaagcggtcgtgcaaccattatgattaaaaaaagcgattttctggccattccgagcgaagaatataaaggtattctgagcctgcgctatcaggtttttaaacagcgtctggaatgggatctggtggttgaaaataatctggaaagtgatgaatatgataatagcaatgccgaatatatttatgcctgtgatgataccgaaaatgttagcggttgttggcgtctgctgccgaccaccggtgattatatgctgaaaagcgtttttccggaactgctgggtcagcagagcgcaccgaaagatccgaatattgttgaactgagccgttttgccgtgggtaaaaatagcagcaaaattaataatagcgccagcgaaattaccatgaaactgtttgaagccatttataaacatgccgttagccagggtattaccgaatatgttaccgttaccagcaccgcaattgaacgttttctgaaacgtattaaagtgccgtgtcatcgtattggcgataaagaaattcatgttctgggcgataccaaaagcgttgttctgagcatgccgattaatgaacagtttaaaaaagccgtgctgaactaataatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaact…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K091166_sequence/1
type: Sequence
persistent identity: BBa K091166 sequence
display id: BBa_K091166_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K199163_sequence/1
type: Sequence
persistent identity: BBa K199163 sequence
display id: BBa_K199163_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatactagatggcgatcgcatctgggcgcgccgcaattgcaagcggtcgtgcaaccattatgattaaaaaaagcgattttctggccattccgagcgaagaatataaaggtattctgagcctgcgctatcaggtttttaaacagcgtctggaatgggatctggtggttgaaaataatctggaaagtgatgaatatgataatagcaatgccgaatatatttatgcctgtgatgataccgaaaatgttagcggttgttggcgtctgctgccgaccaccggtgattatatgctgaaaagcgtttttccggaactgctgggtcagcagagcgcaccgaaagatccgaatattgttgaactgagccgttttgccgtgggtaaaaatagcagcaaaattaataatagcgccagcgaaattaccatgaaactgtttgaagccatttataaacatgccgttagccagggtattaccgaatatgttaccgttaccagcaccgcaattgaacgttttctgaaacgtattaaagtgccgtgtcatcgtattggcgataaagaaattcatgttctgggcgataccaaaagcgttgttctgagcatgccgattaatgaacagtttaaaaaagccgtgctgaactaataatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I13453_sequence/1
type: Sequence
persistent identity: BBa I13453 sequence
display id: BBa_I13453_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J37033_sequence/1
type: Sequence
persistent identity: BBa J37033 sequence
display id: BBa_J37033_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S04419_sequence/1
type: Sequence
persistent identity: BBa S04419 sequence
display id: BBa_S04419_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_S04424_sequence/1
type: Sequence
persistent identity: BBa S04424 sequence
display id: BBa_S04424_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatactagatggcgatcgcatctgggcgcgccgcaattgcaagcggtcgtgcaaccattatgattaaaaaaagcgattttctggccattccgagcgaagaatataaaggtattctgagcctgcgctatcaggtttttaaacagcgtctggaatgggatctggtggttgaaaataatctggaaagtgatgaatatgataatagcaatgccgaatatatttatgcctgtgatgataccgaaaatgttagcggttgttggcgtctgctgccgaccaccggtgattatatgctgaaaagcgtttttccggaactgctgggtcagcagagcgcaccgaaagatccgaatattgttgaactgagccgttttgccgtgggtaaaaatagcagcaaaattaataatagcgccagcgaaattaccatgaaactgtttgaagccatttataaacatgccgttagccagggtattaccgaatatgttaccgttaccagcaccgcaattgaacgttttctgaaacgtattaaagtgccgtgtcatcgtattggcgataaagaaattcatgttctgggcgataccaaaagcgttgttctgagcatgccgattaatgaacagtttaaaaaagccgtgctgaactaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E1010_sequence/1
type: Sequence
persistent identity: BBa E1010 sequence
display id: BBa_E1010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0062_sequence/1
type: Sequence
persistent identity: BBa C0062 sequence
display id: BBa_C0062_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0062_sequence/1
type: Sequence
persistent identity: BBa R0062 sequence
display id: BBa_R0062_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K199130_sequence/1
type: Sequence
persistent identity: BBa K199130 sequence
display id: BBa_K199130_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcgatcgcatctgggcgcgccgcaattgcaagcggtcgtgcaaccattatgattaaaaaaagcgattttctggccattccgagcgaagaatataaaggtattctgagcctgcgctatcaggtttttaaacagcgtctggaatgggatctggtggttgaaaataatctggaaagtgatgaatatgataatagcaatgccgaatatatttatgcctgtgatgataccgaaaatgttagcggttgttggcgtctgctgccgaccaccggtgattatatgctgaaaagcgtttttccggaactgctgggtcagcagagcgcaccgaaagatccgaatattgttgaactgagccgttttgccgtgggtaaaaatagcagcaaaattaataatagcgccagcgaaattaccatgaaactgtttgaagccatttataaacatgccgttagccagggtattaccgaatatgttaccgttaccagcaccgcaattgaacgttttctgaaacgtattaaagtgccgtgtcatcgtattggcgataaagaaattcatgttctgggcgataccaaaagcgttgttctgagcatgccgattaatgaacagtttaaaaaagccgtgctgaactaataa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z