LOCUS       BBa_K199168             1504 bp    DNA     linear       22-Nov-2024
DEFINITION  pT7-RBS-RFP-RBS-GFP
ACCESSION   BBa_K199168
VERSION     BBa_K199168.1  
FEATURES             Location/Qualifiers
     misc_feature    18..18
                     /label=Transcription Start Site
     misc_feature    1..11
                     /label=Polymerase Binding Region
     misc_feature    12..23
                     /label=Initiation Region
     promoter        1..23
                     /label=BBa_R0085
     misc_feature    36..39
                     /label=conserved
     RBS             32..43
                     /label=BBa_B0034
     CDS             50..724
                     /label=mrfp1
     misc_feature    731..755
                     /label=Help:Barcodes
     CDS             50..755
                     /label=BBa_E1010
     misc_feature    1..755
                     /label=BBa_K199118
     RBS             764..778
                     /label=RBS-1\Strong
     RBS             771..775
                     /label=RBS
     misc_feature    764..778
                     /label=BBa_B0030
     RBS             764..778
                     /label=BBa_B0030
     CDS             785..1504
                     /label=GFP protein
     CDS             785..1504
                     /label=BBa_E0040
     misc_feature    764..1504
                     /label=BBa_E5500
ORIGIN
        1 taatacgact cactataggg agatactaga gaaagaggag aaatactaga tggcttcctc
       61 cgaagacgtt atcaaagagt tcatgcgttt caaagttcgt atggaaggtt ccgttaacgg
      121 tcacgagttc gaaatcgaag gtgaaggtga aggtcgtccg tacgaaggta cccagaccgc
      181 taaactgaaa gttaccaaag gtggtccgct gccgttcgct tgggacatcc tgtccccgca
      241 gttccagtac ggttccaaag cttacgttaa acacccggct gacatcccgg actacctgaa
      301 actgtccttc ccggaaggtt tcaaatggga acgtgttatg aacttcgaag acggtggtgt
      361 tgttaccgtt acccaggact cctccctgca agacggtgag ttcatctaca aagttaaact
      421 gcgtggtacc aacttcccgt ccgacggtcc ggttatgcag aaaaaaacca tgggttggga
      481 agcttccacc gaacgtatgt acccggaaga cggtgctctg aaaggtgaaa tcaaaatgcg
      541 tctgaaactg aaagacggtg gtcactacga cgctgaagtt aaaaccacct acatggctaa
      601 aaaaccggtt cagctgccgg gtgcttacaa aaccgacatc aaactggaca tcacctccca
      661 caacgaagac tacaccatcg ttgaacagta cgaacgtgct gaaggtcgtc actccaccgg
      721 tgcttaataa cgctgatagt gctagtgtag atcgctacta gagattaaag aggagaaata
      781 ctagatgcgt aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga
      841 tggtgatgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgcaacata
      901 cggaaaactt acccttaaat ttatttgcac tactggaaaa ctacctgttc catggccaac
      961 acttgtcact actttcggtt atggtgttca atgctttgcg agatacccag atcatatgaa
     1021 acagcatgac tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa gaactatatt
     1081 tttcaaagat gacgggaact acaagacacg tgctgaagtc aagtttgaag gtgataccct
     1141 tgttaataga atcgagttaa aaggtattga ttttaaagaa gatggaaaca ttcttggaca
     1201 caaattggaa tacaactata actcacacaa tgtatacatc atggcagaca aacaaaagaa
     1261 tggaatcaaa gttaacttca aaattagaca caacattgaa gatggaagcg ttcaactagc
     1321 agaccattat caacaaaata ctccaattgg cgatggccct gtccttttac cagacaacca
     1381 ttacctgtcc acacaatctg ccctttcgaa agatcccaac gaaaagagag accacatggt
     1441 ccttcttgag tttgtaacag ctgctgggat tacacatggc atggatgaac tatacaaata
     1501 ataa
//