LOCUS       BBa_K199172             3519 bp    DNA     linear       01-Jun-2024
DEFINITION  T7 Promoter RBS RFP pTet RBS RNAP 2 Sites
ACCESSION   BBa_K199172
VERSION     BBa_K199172.1  
FEATURES             Location/Qualifiers
     misc_feature    18..18
                     /label=Transcription Start Site
     misc_feature    1..11
                     /label=Polymerase Binding Region
     misc_feature    12..23
                     /label=Initiation Region
     promoter        1..23
                     /label=BBa_R0085
     misc_feature    36..39
                     /label=conserved
     RBS             32..43
                     /label=BBa_B0034
     CDS             50..724
                     /label=mrfp1
     misc_feature    731..755
                     /label=Help:Barcodes
     CDS             50..755
                     /label=BBa_E1010
     misc_feature    1..755
                     /label=BBa_K199118
     misc_feature    764..782
                     /label=TetR 1
     misc_feature    764..817
                     /label=BBa_R0040
     promoter        783..788
                     /label=-35
     misc_feature    789..807
                     /label=TetR 2
     promoter        806..811
                     /label=-10
     promoter        764..817
                     /label=BBa_R0040
     misc_feature    830..833
                     /label=conserved
     RBS             826..837
                     /label=BBa_B0034
     promoter        764..837
                     /label=BBa_J13002
     misc_feature    1..837
                     /label=BBa_S04441
     misc_feature    860..867
                     /label=AscI Site
     misc_feature    847..854
                     /label=AsiSI Site
     CDS             844..3519
                     /label=BBa_K199171
ORIGIN
        1 taatacgact cactataggg agatactaga gaaagaggag aaatactaga tggcttcctc
       61 cgaagacgtt atcaaagagt tcatgcgttt caaagttcgt atggaaggtt ccgttaacgg
      121 tcacgagttc gaaatcgaag gtgaaggtga aggtcgtccg tacgaaggta cccagaccgc
      181 taaactgaaa gttaccaaag gtggtccgct gccgttcgct tgggacatcc tgtccccgca
      241 gttccagtac ggttccaaag cttacgttaa acacccggct gacatcccgg actacctgaa
      301 actgtccttc ccggaaggtt tcaaatggga acgtgttatg aacttcgaag acggtggtgt
      361 tgttaccgtt acccaggact cctccctgca agacggtgag ttcatctaca aagttaaact
      421 gcgtggtacc aacttcccgt ccgacggtcc ggttatgcag aaaaaaacca tgggttggga
      481 agcttccacc gaacgtatgt acccggaaga cggtgctctg aaaggtgaaa tcaaaatgcg
      541 tctgaaactg aaagacggtg gtcactacga cgctgaagtt aaaaccacct acatggctaa
      601 aaaaccggtt cagctgccgg gtgcttacaa aaccgacatc aaactggaca tcacctccca
      661 caacgaagac tacaccatcg ttgaacagta cgaacgtgct gaaggtcgtc actccaccgg
      721 tgcttaataa cgctgatagt gctagtgtag atcgctacta gagtccctat cagtgataga
      781 gattgacatc cctatcagtg atagagatac tgagcactac tagagaaaga ggagaaatac
      841 tagatggcga tcgcatctgg gcgcgccaac acgattaaca tcgctaagaa cgacttctct
      901 gacatcgaac tggctgctat cccgttcaac actctggctg accattacgg tgagcgttta
      961 gctcgcgaac agttggccct tgagcatgag tcttacgaga tgggtgaagc acgcttccgc
     1021 aagatgtttg agcgtcaact taaagctggt gaggttgcgg ataacgctgc cgccaagcct
     1081 ctcatcacta ccctactccc taagatgatt gcacgcatca acgactggtt tgaggaagtg
     1141 aaagctaagc gcggcaagcg cccgacagcc ttccagttcc tgcaagaaat caagccggaa
     1201 gccgtagcgt acatcaccat taagaccact ctggcttgcc taaccagtgc tgacaataca
     1261 accgttcagg ctgtagcaag cgcaatcggt cgggccattg aggacgaggc tcgcttcggt
     1321 cgtatccgtg accttgaagc taagcacttc aagaaaaacg ttgaggaaca actcaacaag
     1381 cgcgtagggc acgtctacaa gaaagcattt atgcaagttg tcgaggctga catgctctct
     1441 aagggtctac tcggtggcga ggcgtggtct tcgtggcata aggaagactc tattcatgta
     1501 ggagtacgct gcatcgagat gctcattgag tcaaccggaa tggttagctt acaccgccaa
     1561 aatgctggcg tagtaggtca agactctgag actatcgaac tcgcacctga atacgctgag
     1621 gctatcgcaa cccgtgcagg tgcgctggct ggcatctctc cgatgttcca accttgcgta
     1681 gttcctccta agccgtggac tggcattact ggtggtggct attgggctaa cggtcgtcgt
     1741 cctctggcgc tggtgcgtac tcacagtaag aaagcactga tgcgctacga agacgtttac
     1801 atgcctgagg tgtacaaagc gattaacatt gcgcaaaaca ccgcatggaa aatcaacaag
     1861 aaagtcctag cggtcgccaa cgtaatcacc aagtggaagc attgtccggt cgaggacatc
     1921 cctgcgattg agcgtgaaga actcccgatg aaaccggaag acatcgacat gaatcctgag
     1981 gctctcaccg cgtggaaacg tgctgccgct gctgtgtacc gcaaggacaa ggctcgcaag
     2041 tctcgccgta tcagccttga gttcatgctt gagcaagcca ataagtttgc taaccataag
     2101 gccatctggt tcccttacaa catggactgg cgcggtcgtg tttacgctgt gtcaatgttc
     2161 aacccgcaag gtaacgatat gaccaaagga ctgcttacgc tggcgaaagg taaaccaatc
     2221 ggtaaggaag gttactactg gctgaaaatc cacggtgcaa actgtgcggg tgtcgataag
     2281 gttccgttcc ctgagcgcat caagttcatt gaggaaaacc acgagaacat catggcttgc
     2341 gctaagtctc cactggagaa cacttggtgg gctgagcaag attctccgtt ctgcttcctt
     2401 gcgttctgct ttgagtacgc tggggtacag caccacggcc tgagctataa ctgctccctt
     2461 ccgctggcgt ttgacgggtc ttgctctggc atccagcact tctccgcgat gctccgagat
     2521 gaggtaggtg gtcgcgcggt taacttgctt cctagtgaaa ccgttcagga catctacggg
     2581 attgttgcta agaaagtcaa cgagattcta caagcagacg caatcaatgg gaccgataac
     2641 gaagtagtta ccgtgaccga tgagaacact ggtgaaatct ctgagaaagt caagctgggc
     2701 actaaggcac tggctggtca atggctggct tacggtgtta ctcgcagtgt gactaagcgt
     2761 tcagtcatga cgctggctta cgggtccaaa gagttcggct tccgtcaaca agtgctggaa
     2821 gataccattc agccagctat tgattccggc aagggtctga tgttcactca gccgaatcag
     2881 gctgctggat acatggctaa gctgatttgg gaatctgtga gcgtgacggt ggtagctgcg
     2941 gttgaagcaa tgaactggct taagtctgct gctaagctgc tggctgctga ggtcaaagat
     3001 aagaagactg gagagattct tcgcaagcgt tgcgctgtgc attgggtaac tcctgatggt
     3061 ttccctgtgt ggcaggaata caagaagcct attcagacgc gcttgaacct gatgttcctc
     3121 ggtcagttcc gcttacagcc taccattaac accaacaaag atagcgagat tgatgcacac
     3181 aaacaggagt ctggtatcgc tcctaacttt gtacacagcc aagacggtag ccaccttcgt
     3241 aagactgtag tgtgggcaca cgagaagtac ggaatcgaat cttttgcact gattcacgac
     3301 tccttcggta ccattccggc tgacgctgcg aacctgttca aagcagtgcg cgaaactatg
     3361 gttgacacat atgagtcttg tgatgtactg gctgatttct acgaccagtt cgctgaccag
     3421 ttgcacgagt ctcaattgga caaaatgcca gcacttccgg ctaaaggtaa cttgaacctc
     3481 cgtgacatct tagagtcgga cttcgcgttc gcgtaataa
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