LOCUS       BBa_K199173             1876 bp    DNA     linear       29-Dec-2025
DEFINITION  RBS RFP pLac RBS LUXR Double Terminator
ACCESSION   BBa_K199173
VERSION     BBa_K199173.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..693
                     /label=mrfp1
     misc_feature    700..724
                     /label=Help:Barcodes
     CDS             19..724
                     /label=BBa_E1010
     misc_feature    1..724
                     /label=BBa_S04419
     CDS             733..820
                     /label=end of LacI coding region (inactive)
     misc_feature    733..932
                     /label=BBa_R0010
     misc_feature    821..858
                     /label=CAP binding site
     promoter        869..874
                     /label=-35
     promoter        893..898
                     /label=-10
     misc_feature    898..932
                     /label=LacI binding site
     misc_feature    905..905
                     /label=start
     promoter        733..932
                     /label=BBa_R0010
     misc_feature    945..948
                     /label=conserved
     RBS             941..952
                     /label=BBa_B0034
     misc_feature    959..960
                     /label=prefix
     misc_feature    1132..1132
                     /label=T
     misc_feature    1450..1450
                     /label=A
     CDS             959..1708
                     /label=luxR
     misc_feature    959..1714
                     /label=BBa_C0062
     misc_feature    1715..1739
                     /label=Help:Barcodes
     CDS             959..1714
                     /label=BBa_C0062
     stem_loop       1759..1802
                     /label=stem_loop
     misc_feature    1748..1827
                     /label=BBa_B0010
     terminator      1748..1827
                     /label=BBa_B0010
     stem_loop       1843..1862
                     /label=T7 TE
     misc_feature    1869..1869
                     /label=stop
     polyA_site      1863..1876
                     /label=polya
     misc_feature    1836..1876
                     /label=BBa_B0012
     terminator      1836..1876
                     /label=BBa_B0012
     misc_feature    941..1876
                     /label=BBa_I0462
     misc_feature    733..1876
                     /label=BBa_K109702
ORIGIN
        1 aaagaggaga aatactagat ggcttcctcc gaagacgtta tcaaagagtt catgcgtttc
       61 aaagttcgta tggaaggttc cgttaacggt cacgagttcg aaatcgaagg tgaaggtgaa
      121 ggtcgtccgt acgaaggtac ccagaccgct aaactgaaag ttaccaaagg tggtccgctg
      181 ccgttcgctt gggacatcct gtccccgcag ttccagtacg gttccaaagc ttacgttaaa
      241 cacccggctg acatcccgga ctacctgaaa ctgtccttcc cggaaggttt caaatgggaa
      301 cgtgttatga acttcgaaga cggtggtgtt gttaccgtta cccaggactc ctccctgcaa
      361 gacggtgagt tcatctacaa agttaaactg cgtggtacca acttcccgtc cgacggtccg
      421 gttatgcaga aaaaaaccat gggttgggaa gcttccaccg aacgtatgta cccggaagac
      481 ggtgctctga aaggtgaaat caaaatgcgt ctgaaactga aagacggtgg tcactacgac
      541 gctgaagtta aaaccaccta catggctaaa aaaccggttc agctgccggg tgcttacaaa
      601 accgacatca aactggacat cacctcccac aacgaagact acaccatcgt tgaacagtac
      661 gaacgtgctg aaggtcgtca ctccaccggt gcttaataac gctgatagtg ctagtgtaga
      721 tcgctactag agcaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
      781 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
      841 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
      901 gtggaattgt gagcggataa caatttcaca catactagag aaagaggaga aatactagat
      961 gaaaaacata aatgccgacg acacatacag aataattaat aaaattaaag cttgtagaag
     1021 caataatgat attaatcaat gcttatctga tatgactaaa atggtacatt gtgaatatta
     1081 tttactcgcg atcatttatc ctcattctat ggttaaatct gatatttcaa tcctagataa
     1141 ttaccctaaa aaatggaggc aatattatga tgacgctaat ttaataaaat atgatcctat
     1201 agtagattat tctaactcca atcattcacc aattaattgg aatatatttg aaaacaatgc
     1261 tgtaaataaa aaatctccaa atgtaattaa agaagcgaaa acatcaggtc ttatcactgg
     1321 gtttagtttc cctattcata cggctaacaa tggcttcgga atgcttagtt ttgcacattc
     1381 agaaaaagac aactatatag atagtttatt tttacatgcg tgtatgaaca taccattaat
     1441 tgttccttct ctagttgata attatcgaaa aataaatata gcaaataata aatcaaacaa
     1501 cgatttaacc aaaagagaaa aagaatgttt agcgtgggca tgcgaaggaa aaagctcttg
     1561 ggatatttca aaaatattag gttgcagtga gcgtactgtc actttccatt taaccaatgc
     1621 gcaaatgaaa ctcaatacaa caaaccgctg ccaaagtatt tctaaagcaa ttttaacagg
     1681 agcaattgat tgcccatact ttaaaaatta ataacactga tagtgctagt gtagatcact
     1741 actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
     1801 atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg
     1861 cctttctgcg tttata
//