##gff-version 3
##sequence-region BBa_K199173 1 1876
BBa_K199173	.	engineered_region	733	1876	.	+	0	ID=BBa_K109702;Name=BBa_K109702
BBa_K199173	.	promoter	733	932	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_K109702
BBa_K199173	.	start_codon	905	905	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K199173	.	CDS	733	820	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K199173	.	promoter	893	898	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K199173	.	non_covalent_binding_site	898	932	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K199173	.	non_covalent_binding_site	821	858	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K199173	.	engineered_region	733	932	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K199173	.	promoter	869	874	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K199173	.	engineered_region	941	1876	.	+	0	ID=BBa_I0462;Name=BBa_I0462;Parent=BBa_K109702
BBa_K199173	.	terminator	1748	1827	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0462
BBa_K199173	.	stem_loop	1759	1802	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K199173	.	engineered_region	1748	1827	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K199173	.	terminator	1836	1876	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0462
BBa_K199173	.	polyA_site	1863	1876	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K199173	.	engineered_region	1836	1876	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K199173	.	stem_loop	1843	1862	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K199173	.	stop_codon	1869	1869	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K199173	.	ribosome_entry_site	941	952	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0462
BBa_K199173	.	conserved_region	945	948	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K199173	.	CDS	959	1714	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_I0462
BBa_K199173	.	CDS	959	1708	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K199173	.	engineered_tag	1715	1739	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K199173	.	engineered_region	959	1714	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K199173	.	point_mutation	1132	1132	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K199173	.	engineered_region	959	960	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K199173	.	point_mutation	1450	1450	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K199173	.	engineered_region	1	724	.	+	0	ID=BBa_S04419;Name=BBa_S04419
BBa_K199173	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S04419
BBa_K199173	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K199173	.	CDS	19	724	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_S04419
BBa_K199173	.	CDS	19	693	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K199173	.	engineered_tag	700	724	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
>BBa_K199173
aaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggtt
ccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaa
agttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgtt
aaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcg
aagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtgg
taccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccg
gaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaa
ccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaa
cgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgc
tagtgtagatcgctactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacga
caggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggct
ttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagagga
gaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataat
gatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcatt
ctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaat
aaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgta
aataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacgg
ctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtg
tatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaac
gatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattag
gttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtat
ttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatca
ctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggt
gaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata