##gff-version 3
##sequence-region BBa_K199174 1 1586
BBa_K199174	.	engineered_region	1	1504	.	+	0	ID=BBa_K199168;Name=BBa_K199168
BBa_K199174	.	engineered_region	1	755	.	+	0	ID=BBa_K199118;Name=BBa_K199118;Parent=BBa_K199168
BBa_K199174	.	CDS	50	755	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_K199118
BBa_K199174	.	CDS	50	724	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K199174	.	engineered_tag	731	755	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K199174	.	ribosome_entry_site	32	43	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K199118
BBa_K199174	.	conserved_region	36	39	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K199174	.	promoter	1	23	.	+	0	ID=BBa_R0085;Name=BBa_R0085;Parent=BBa_K199118
BBa_K199174	.	non_covalent_binding_site	1	11	.	+	0	ID=annotation1721521;Name=Polymerase Binding Region;Parent=BBa_R0085
BBa_K199174	.	sequence_feature	18	18	.	+	0	ID=annotation1721520;Name=Transcription Start Site;Parent=BBa_R0085
BBa_K199174	.	sequence_feature	12	23	.	+	0	ID=annotation1721522;Name=Initiation Region;Parent=BBa_R0085
BBa_K199174	.	engineered_region	764	1504	.	+	0	ID=BBa_E5500;Name=BBa_E5500;Parent=BBa_K199168
BBa_K199174	.	ribosome_entry_site	764	778	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_E5500
BBa_K199174	.	engineered_region	764	778	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K199174	.	ribosome_entry_site	771	775	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K199174	.	ribosome_entry_site	764	778	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K199174	.	CDS	785	1504	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_E5500
BBa_K199174	.	CDS	785	1504	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K199174	.	promoter	1513	1586	.	+	0	ID=BBa_J13002;Name=BBa_J13002
BBa_K199174	.	promoter	1513	1566	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_J13002
BBa_K199174	.	promoter	1532	1537	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K199174	.	non_covalent_binding_site	1538	1556	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K199174	.	engineered_region	1513	1566	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K199174	.	non_covalent_binding_site	1513	1531	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K199174	.	promoter	1555	1560	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K199174	.	ribosome_entry_site	1575	1586	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J13002
BBa_K199174	.	conserved_region	1579	1582	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_K199174
taatacgactcactatagggagatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagag
ttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtc
cgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtcccc
gcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaa
ggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacg
gtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatggg
ttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaa
gacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaa
ccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtca
ctccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagattaaagaggagaaatactagatgcgt
aaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctg
tcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgt
tccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcat
gactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaaga
cacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatgg
aaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatgga
atcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactc
caattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacga
aaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataa
taatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagag
gagaaa