LOCUS       BBa_K199175             1893 bp    DNA     linear       22-Dec-2024
DEFINITION  RBS-GFP-pLac-RBS-LuxR-TT
ACCESSION   BBa_K199175
VERSION     BBa_K199175.1  
FEATURES             Location/Qualifiers
     RBS             1..15
                     /label=RBS-1\Strong
     RBS             8..12
                     /label=RBS
     misc_feature    1..15
                     /label=BBa_B0030
     RBS             1..15
                     /label=BBa_B0030
     CDS             22..741
                     /label=GFP protein
     CDS             22..741
                     /label=BBa_E0040
     misc_feature    1..741
                     /label=BBa_E5500
     CDS             750..837
                     /label=end of LacI coding region (inactive)
     misc_feature    750..949
                     /label=BBa_R0010
     misc_feature    838..875
                     /label=CAP binding site
     promoter        886..891
                     /label=-35
     promoter        910..915
                     /label=-10
     misc_feature    915..949
                     /label=LacI binding site
     misc_feature    922..922
                     /label=start
     promoter        750..949
                     /label=BBa_R0010
     misc_feature    962..965
                     /label=conserved
     RBS             958..969
                     /label=BBa_B0034
     misc_feature    976..977
                     /label=prefix
     misc_feature    1149..1149
                     /label=T
     misc_feature    1467..1467
                     /label=A
     CDS             976..1725
                     /label=luxR
     misc_feature    976..1731
                     /label=BBa_C0062
     misc_feature    1732..1756
                     /label=Help:Barcodes
     CDS             976..1731
                     /label=BBa_C0062
     stem_loop       1776..1819
                     /label=stem_loop
     misc_feature    1765..1844
                     /label=BBa_B0010
     terminator      1765..1844
                     /label=BBa_B0010
     stem_loop       1860..1879
                     /label=T7 TE
     misc_feature    1886..1886
                     /label=stop
     polyA_site      1880..1893
                     /label=polya
     misc_feature    1853..1893
                     /label=BBa_B0012
     terminator      1853..1893
                     /label=BBa_B0012
     misc_feature    958..1893
                     /label=BBa_I0462
     misc_feature    750..1893
                     /label=BBa_K109702
ORIGIN
        1 attaaagagg agaaatacta gatgcgtaaa ggagaagaac ttttcactgg agttgtccca
       61 attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt
      121 gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac tggaaaacta
      181 cctgttccat ggccaacact tgtcactact ttcggttatg gtgttcaatg ctttgcgaga
      241 tacccagatc atatgaaaca gcatgacttt ttcaagagtg ccatgcccga aggttatgta
      301 caggaaagaa ctatattttt caaagatgac gggaactaca agacacgtgc tgaagtcaag
      361 tttgaaggtg atacccttgt taatagaatc gagttaaaag gtattgattt taaagaagat
      421 ggaaacattc ttggacacaa attggaatac aactataact cacacaatgt atacatcatg
      481 gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa cattgaagat
      541 ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc
      601 cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga tcccaacgaa
      661 aagagagacc acatggtcct tcttgagttt gtaacagctg ctgggattac acatggcatg
      721 gatgaactat acaaataata atactagagc aatacgcaaa ccgcctctcc ccgcgcgttg
      781 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
      841 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
      901 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacat actagagaaa
      961 gaggagaaat actagatgaa aaacataaat gccgacgaca catacagaat aattaataaa
     1021 attaaagctt gtagaagcaa taatgatatt aatcaatgct tatctgatat gactaaaatg
     1081 gtacattgtg aatattattt actcgcgatc atttatcctc attctatggt taaatctgat
     1141 atttcaatcc tagataatta ccctaaaaaa tggaggcaat attatgatga cgctaattta
     1201 ataaaatatg atcctatagt agattattct aactccaatc attcaccaat taattggaat
     1261 atatttgaaa acaatgctgt aaataaaaaa tctccaaatg taattaaaga agcgaaaaca
     1321 tcaggtctta tcactgggtt tagtttccct attcatacgg ctaacaatgg cttcggaatg
     1381 cttagttttg cacattcaga aaaagacaac tatatagata gtttattttt acatgcgtgt
     1441 atgaacatac cattaattgt tccttctcta gttgataatt atcgaaaaat aaatatagca
     1501 aataataaat caaacaacga tttaaccaaa agagaaaaag aatgtttagc gtgggcatgc
     1561 gaaggaaaaa gctcttggga tatttcaaaa atattaggtt gcagtgagcg tactgtcact
     1621 ttccatttaa ccaatgcgca aatgaaactc aatacaacaa accgctgcca aagtatttct
     1681 aaagcaattt taacaggagc aattgattgc ccatacttta aaaattaata acactgatag
     1741 tgctagtgta gatcactact agagccaggc atcaaataaa acgaaaggct cagtcgaaag
     1801 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg
     1861 gctcaccttc gggtgggcct ttctgcgttt ata
//